hph

UniProt ID: P00557
Organism: Escherichia coli
Review Status: DRAFT
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Gene Description

hph encodes an aminoglycoside phosphotransferase in Escherichia coli. UniProt/CARD identify this entry as an aminoglycoside-modifying resistance enzyme that inactivates aminoglycosides by phosphorylation. UniProt names the protein 'Hygromycin-B 4-O-kinase' (accession P00557).

Existing Annotations Review

GO Term Evidence Action Reason
GO:0034071 aminoglycoside phosphotransferase activity
RCA
file:projects/ANTIMICROBIAL_RESISTANCE/aro2go.sssom.yaml 		NEW
Summary: NEW candidate annotation from the curated ARO->GO mapping: aminoglycoside phosphotransferase activity.
Reason: The UniProt record cross-references a CARD/ARO AMR determinant, and the curated ARO->GO mapping projects the family-specific molecular function aminoglycoside phosphotransferase activity. This focused draft accepts the candidate as a curator lead for this AMR determinant.
Supporting Evidence:
file:projects/ANTIMICROBIAL_RESISTANCE/data/candidate_new_annotations.tsv
P00557 ARO:3002655 ARO:3000114 narrower RO:0002327 GO:0034071 aminoglycoside phosphotransferase activity
file:genes/ECOLX/hph/hph-uniprot.txt
CC -!- FUNCTION: The aminoglycoside phosphotransferases achieve inactivation CC of their antibiotic substrates by phosphorylation. Only phosphorylates CC hygromycin and closely related compounds such as demethyl analogs and
GO:0046677 response to antibiotic
RCA
file:projects/ANTIMICROBIAL_RESISTANCE/aro2go.sssom.yaml 		NEW
Summary: NEW process annotation: hph is an AMR determinant involved in antibiotic response/resistance.
Reason: CARD/ARO identity places this gene in an antimicrobial-resistance determinant family; response to antibiotic is the appropriate high-level GO biological process for the resistance role.
Supporting Evidence:
file:genes/ECOLX/hph/hph-uniprot.txt
DR CARD; ARO:3002655; APH(4)-Ia; ARO:0001004; antibiotic inactivation.

Core Functions

hph phosphorylates aminoglycoside antibiotics, reducing drug activity and contributing to antibiotic resistance.

Molecular Function:
aminoglycoside phosphotransferase activity
Directly Involved In:
response to antibiotic
Supporting Evidence:
  • file:genes/ECOLX/hph/hph-uniprot.txt
    CC -!- FUNCTION: The aminoglycoside phosphotransferases achieve inactivation CC of their antibiotic substrates by phosphorylation. Only phosphorylates CC hygromycin and closely related compounds such as demethyl analogs and

References

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Suggested Questions for Experts

Q: Is the ARO-derived aminoglycoside phosphotransferase activity annotation sufficiently specific for hph, or is a narrower substrate/site-specific GO term warranted?

Suggested Experiments

Experiment: Biochemically assay purified hph against representative antibiotic substrates for the inferred AMR family and measure loss of drug activity or target modification.

Type: in vitro enzyme assay

📚 Additional Documentation

Notes

(hph-notes.md)

Notes: hph

Focused AMR batch review. UniProt accession: P00557. Source organism: Escherichia coli.

  • UniProt/CARD provenance: DR CARD; ARO:3002655; APH(4)-Ia; ARO:0001004; antibiotic inactivation.
  • ARO-to-GO candidate: GO:0034071 (aminoglycoside phosphotransferase activity) from projects/ANTIMICROBIAL_RESISTANCE/data/candidate_new_annotations.tsv and projects/ANTIMICROBIAL_RESISTANCE/aro2go.sssom.yaml.
  • This is a DRAFT focused review intended to cover the AMR annotation gap; it has not had a gene-specific deep-research pass.

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