id: A0A0M5JP06
gene_symbol: rmtE
product_type: PROTEIN
status: DRAFT
taxon:
  id: NCBITaxon:562
  label: Escherichia coli
description: rmtE encodes a 16S rRNA G1405 N7 methyltransferase in Escherichia coli. UniProt/CARD identify this entry as an acquired aminoglycoside-resistance methyltransferase; methylation of 16S rRNA protects the ribosomal A site from clinically important aminoglycosides. UniProt names the protein '16S rRNA (guanine(1405)-N(7))-methyltransferase' (accession A0A0M5JP06).
existing_annotations:
- term:
    id: GO:0008649
    label: rRNA methyltransferase activity
  evidence_type: IEA
  original_reference_id: GO_REF:0000120
  qualifier: enables
  review:
    summary: The existing MF term is directionally correct but less informative than the family-specific AMR activity rRNA (guanine-N7-)-methyltransferase activity.
    action: MODIFY
    reason: Replace the broad or over-specific electronic term 'rRNA methyltransferase activity' with rRNA (guanine-N7-)-methyltransferase activity based on UniProt/CARD determinant identity and the curated ARO->GO mapping.
    proposed_replacement_terms:
    - id: GO:0070043
      label: rRNA (guanine-N7-)-methyltransferase activity
    supported_by:
    - reference_id: file:genes/ECOLX/rmtE/rmtE-uniprot.txt
      supporting_text: 'CC   -!- FUNCTION: Specifically methylates the N7 position of guanine 1405 in

        CC       16S rRNA. Confers resistance to various aminoglycosides.

        CC       {ECO:0000256|PIRNR:PIRNR015852}.'
- term:
    id: GO:0046677
    label: response to antibiotic
  evidence_type: IEA
  original_reference_id: GO_REF:0000120
  qualifier: involved_in
  review:
    summary: The gene product is an antimicrobial-resistance determinant, so the antibiotic-response annotation is retained.
    action: ACCEPT
    reason: The CARD/ARO determinant identity supports an antibiotic-resistance biological role.
    supported_by:
    - reference_id: file:genes/ECOLX/rmtE/rmtE-uniprot.txt
      supporting_text: 'CC   -!- FUNCTION: Specifically methylates the N7 position of guanine 1405 in

        CC       16S rRNA. Confers resistance to various aminoglycosides.

        CC       {ECO:0000256|PIRNR:PIRNR015852}.'
- term:
    id: GO:0070043
    label: rRNA (guanine-N7-)-methyltransferase activity
  evidence_type: RCA
  original_reference_id: file:projects/ANTIMICROBIAL_RESISTANCE/aro2go.sssom.yaml
  qualifier: enables
  review:
    summary: 'NEW candidate annotation from the curated ARO->GO mapping: rRNA (guanine-N7-)-methyltransferase activity.'
    action: NEW
    reason: The UniProt record cross-references a CARD/ARO AMR determinant, and the curated ARO->GO mapping projects the family-specific molecular function rRNA (guanine-N7-)-methyltransferase activity. This focused draft accepts the candidate as a curator lead for this AMR determinant.
    additional_reference_ids:
    - file:projects/ANTIMICROBIAL_RESISTANCE/data/candidate_new_annotations.tsv
    - file:genes/ECOLX/rmtE/rmtE-uniprot.txt
    supported_by:
    - reference_id: file:projects/ANTIMICROBIAL_RESISTANCE/data/candidate_new_annotations.tsv
      supporting_text: "A0A0M5JP06\tARO:3004679\tARO:3004271\tnarrower\tskos:relatedMatch\tGO:0070043\trRNA (guanine-N7-)-methyltransferase activity"
    - reference_id: file:genes/ECOLX/rmtE/rmtE-uniprot.txt
      supporting_text: 'CC   -!- FUNCTION: Specifically methylates the N7 position of guanine 1405 in

        CC       16S rRNA. Confers resistance to various aminoglycosides.

        CC       {ECO:0000256|PIRNR:PIRNR015852}.'
references:
- id: GO_REF:0000120
  title: Combined Automated Annotation using Multiple IEA Methods
  findings: []
- id: file:genes/ECOLX/rmtE/rmtE-uniprot.txt
  title: UniProt flat file for rmtE (A0A0M5JP06)
  findings:
  - statement: UniProt/CARD identity and protein naming for rmtE.
    supporting_text: 'CC   -!- FUNCTION: Specifically methylates the N7 position of guanine 1405 in

      CC       16S rRNA. Confers resistance to various aminoglycosides.

      CC       {ECO:0000256|PIRNR:PIRNR015852}.'
- id: file:projects/ANTIMICROBIAL_RESISTANCE/aro2go.sssom.yaml
  title: Curated ARO to GO mapping set for AMR gene families
  findings:
  - statement: Manual ARO-to-GO mappings used to identify focused AMR annotation gains.
    supporting_text: 'mapping_set_title: ARO to GO mapping (AMR gene families and resistance mechanisms)'
- id: file:projects/ANTIMICROBIAL_RESISTANCE/data/candidate_new_annotations.tsv
  title: Annotation-gain candidates from ARO to GO mappings
  findings:
  - statement: Candidate GO annotations projected from the ARO-to-GO mapping set.
    supporting_text: Candidate GO annotations that UniProtKB entries with a CARD cross-reference would gain from the
core_functions:
- description: rmtE methylates 16S rRNA at guanine N7 in the ribosome, protecting the aminoglycoside binding site and contributing to antibiotic resistance.
  molecular_function:
    id: GO:0070043
    label: rRNA (guanine-N7-)-methyltransferase activity
  directly_involved_in:
  - id: GO:0046677
    label: response to antibiotic
  supported_by:
  - reference_id: file:genes/ECOLX/rmtE/rmtE-uniprot.txt
    supporting_text: 'CC   -!- FUNCTION: Specifically methylates the N7 position of guanine 1405 in

      CC       16S rRNA. Confers resistance to various aminoglycosides.

      CC       {ECO:0000256|PIRNR:PIRNR015852}.'
suggested_questions:
- question: Is the ARO-derived rRNA (guanine-N7-)-methyltransferase activity annotation sufficiently specific for rmtE, or is a narrower substrate/site-specific GO term warranted?
  experts: []
suggested_experiments:
- description: Biochemically assay purified rmtE against representative antibiotic substrates for the inferred AMR family and measure loss of drug activity or target modification.
  experiment_type: in vitro enzyme assay