flbB

Existing Annotations Review

GO Term	Evidence	Action	Reason
GO:0000976 transcription cis-regulatory region binding	IBA GO_REF:0000033	ACCEPT	Summary: Accept: transcription cis-regulatory region binding. Reason: bZIP factors bind cis-regulatory DNA as dimers, and the family-level call matches FlbB's conserved basic region; the promoter FlbB is required to activate in this cascade is brlA.
GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific	IBA GO_REF:0000033	ACCEPT	Summary: Accept: DNA-binding transcription activator activity, RNA polymerase II-specific. Reason: The activator direction is the right one for FlbB: it is required to induce brlA rather than to repress it, so the signed Pol II activator term is the informative molecular function.
GO:0090575 RNA polymerase II transcription regulator complex	IBA GO_REF:0000033	KEEP AS NON CORE	Summary: Non-core. Family-level IBA complex inference with no direct complex evidence for FlbB. Reason: This is a part_of complex assertion inherited by IBA from the bZIP family tree; no A. nidulans experiment cited in this review places FlbB in a Pol II transcription regulator complex. Plausible for a bZIP, which acts as a dimer, but it is not the demonstrated core function and should not be read as complex evidence.
GO:0000976 transcription cis-regulatory region binding	IEA GO_REF:0000002	ACCEPT	Summary: Accept: transcription cis-regulatory region binding. Reason: Reaches the same cis-regulatory-binding statement as the IBA row from sequence similarity. Redundant, but not incorrect.
GO:0003700 DNA-binding transcription factor activity	IEA GO_REF:0000002	ACCEPT	Summary: Accept: DNA-binding transcription factor activity. Reason: The general TF term, subsumed by the Pol II-specific activator call above; kept as its unsigned parent.
GO:0005634 nucleus	IEA GO_REF:0000044	ACCEPT	Summary: Accept: nucleus. Reason: Electronic location call. FlbB's nuclear pool is independently established by the IDA rows in this review, and its unusual second pool at the hyphal tip is annotated separately.
GO:0006355 regulation of DNA-templated transcription	IEA GO_REF:0000002	ACCEPT	Summary: Accept: regulation of DNA-templated transcription. Reason: Generic transcription-regulation parent. The Pol II-specific and conidiation-specific terms in this review carry the actual content.
GO:0045944 positive regulation of transcription by RNA polymerase II	IEA GO_REF:0000108	ACCEPT	Summary: Accept: positive regulation of transcription by RNA polymerase II. Reason: Signed and Pol II-specific, matching FlbB's activating role on brlA; the most informative of the electronic process terms here.
GO:0048315 conidium formation	IMP PMID:17993569 Basic-zipper-type transcription factor FlbB controls asexual...	ACCEPT	Summary: Accept: conidium formation. Reason: flbB loss of function produces the fluffy phenotype that named the gene, so involvement in conidium formation is directly supported by mutant evidence.
GO:0048315 conidium formation	IMP PMID:21423749 Elucidation of functional markers from Aspergillus nidulans ...	ACCEPT	Summary: Accept: conidium formation. Reason: Independent mutational replication of the conidiation requirement, using the domain and marker variants of FlbB characterised in this study; the same term from a separate experiment.
GO:0006357 regulation of transcription by RNA polymerase II	IMP PMID:21624998 Regulation of conidiation by light in Aspergillus nidulans.	ACCEPT	Summary: Accept: regulation of transcription by RNA polymerase II. Reason: FlbB is required for normal developmental transcription in this light-regulated conidiation study. Pol II-specific regulation is the right level for a bZIP acting on brlA.
GO:0075307 positive regulation of conidium formation	IMP PMID:21423749 Elucidation of functional markers from Aspergillus nidulans ...	ACCEPT	Summary: Accept: positive regulation of conidium formation. Reason: The signed, process-specific term and the single best statement of FlbB's biological role: it is an activator, not a repressor, of conidiation.
GO:0005634 nucleus	IDA PMID:20132447 The concerted action of bZip and cMyb transcription factors ...	ACCEPT	Summary: Accept: nucleus. Reason: Direct localisation in the study of concerted FlbB/FlbD action on brlA. The nucleus is where FlbB's transcriptional function is executed.
GO:0001411 hyphal tip	IDA PMID:19656299 The bZIP-type transcription factor FlbB regulates distinct m...	ACCEPT	Summary: Hyphal-tip localization (distinctive). Reason: FlbB shows characteristic hyphal-tip localization in addition to nuclear localization. Supporting Evidence: PMID:19656299 FlbB was located at the tip of growing metulae, following a similar pattern as described in vegetative hyphae. PMID:17993569 FlbB accumulates at the hyphal apex during early vegetative growth but is later found in apical nuclei, suggesting that an activating modification triggers nuclear import.
GO:0003677 DNA binding	IDA PMID:19656299 The bZIP-type transcription factor FlbB regulates distinct m...	ACCEPT	Summary: Core molecular function. FlbB binds DNA via its bZIP domain. Reason: Deferring to the curator's IDA. Note that the cached record for PMID:19656299 is abstract-only, so the binding assay itself is not visible here; both cached papers describe FlbB as a putative bZIP factor, and the corroboration available to this review is the conserved N-terminal bZIP domain rather than a quotable binding experiment. Supporting Evidence: PMID:17993569 In this report we describe FlbB as a putative basic-zipper-type transcription factor restricted to filamentous fungi.
GO:0005634 nucleus	IDA PMID:19656299 The bZIP-type transcription factor FlbB regulates distinct m...	ACCEPT	Summary: Accept: nucleus. Reason: A second direct nuclear localisation, from an independent study of FlbB's staged role in colony morphogenesis.
GO:0001411 hyphal tip	IDA PMID:17993569 Basic-zipper-type transcription factor FlbB controls asexual...	ACCEPT	Summary: Accept: hyphal tip. Reason: FlbB's apical pool, which is unusual for a transcription factor and one of the distinctive facts about this protein. Kept alongside the nuclear location rather than in place of it.
GO:0001411 hyphal tip	IDA PMID:19007409 Aspergillus nidulans FlbE is an upstream developmental activ...	ACCEPT	Summary: Accept: hyphal tip. Reason: Independent direct evidence for the apical pool from the FlbE study, where tip localisation is the property shared by the two upstream activators.
GO:0001411 hyphal tip	IPI PMID:19007409 Aspergillus nidulans FlbE is an upstream developmental activ...	ACCEPT	Summary: Accept: hyphal tip. Reason: The same apical location reached through the FlbE interaction; the interaction-based route is what ties FlbB's tip pool to its upstream partner.
GO:0003700 DNA-binding transcription factor activity	ISM PMID:17993569 Basic-zipper-type transcription factor FlbB controls asexual...	ACCEPT	Summary: Accept: DNA-binding transcription factor activity. Reason: Sequence-model evidence for the bZIP domain. Both cached papers describe FlbB as a putative bZIP factor, so the domain assignment is solid while a direct DNA-binding assay is not visible in this review.
GO:0003700 DNA-binding transcription factor activity	IDA PMID:17993569 Basic-zipper-type transcription factor FlbB controls asexual...	ACCEPT	Summary: Core molecular function. DNA-binding transcription factor activity. Reason: FlbB is a bZIP-family factor whose nuclear import precedes, and is required for, brlA transcription — the behaviour of a transcriptional activator. The cached text describes the domain and the brlA requirement rather than an in vitro binding assay, so this rests on the curator's IDA plus domain and genetic evidence. Supporting Evidence: PMID:17993569 In this report we describe FlbB as a putative basic-zipper-type transcription factor restricted to filamentous fungi. PMID:17993569 proper temporal and quantitative expression of FlbB is a prerequisite for brlA transcription, and misscheduled overexpression inhibits conidiation
GO:0005634 nucleus	IDA PMID:17993569 Basic-zipper-type transcription factor FlbB controls asexual...	ACCEPT	Summary: Accept: nucleus. Reason: A third direct nuclear localisation, from the paper that first characterised FlbB; the three studies agree.
GO:0006357 regulation of transcription by RNA polymerase II	ISM PMID:17993569 Basic-zipper-type transcription factor FlbB controls asexual...	ACCEPT	Summary: Accept: regulation of transcription by RNA polymerase II. Reason: Inferred from the bZIP architecture rather than from a transcription assay. Weaker than the IMP row for the same term, but concordant with it.
GO:0006357 regulation of transcription by RNA polymerase II	IDA PMID:17993569 Basic-zipper-type transcription factor FlbB controls asexual...	ACCEPT	Summary: Accept: regulation of transcription by RNA polymerase II. Reason: Direct evidence that FlbB affects developmental transcription; together with the brlA induction data this is the mechanistic core of the annotation set.
GO:0075307 positive regulation of conidium formation	IMP PMID:17993569 Basic-zipper-type transcription factor FlbB controls asexual...	ACCEPT	Summary: Core process. FlbB positively regulates conidium formation. Reason: flbB mutants are delayed/deficient in conidiation; FlbB is a positive regulator. Supporting Evidence: PMID:17993569 proper temporal and quantitative expression of FlbB is a prerequisite for brlA transcription, and misscheduled overexpression inhibits conidiation

Gene Description

Existing Annotations Review

Core Functions

Upstream bZIP transcription factor activating conidiation: FlbB binds DNA and, through its hyphal-tip-to-nucleus localization, drives timely induction of brlA and the conidiation program, acting with FlbE, FlbC and FlbD.

References

📚 Additional Documentation

Notes

flbB (Aspergillus nidulans) — curation notes

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