flbD encodes a Myb-like DNA-binding transcription factor that is an upstream developmental activator coordinating the initiation of conidiophore development in Aspergillus nidulans, and is uniquely required for both asexual (conidiation) and sexual (cleistothecium/ascospore) differentiation. FlbD acts with FlbB upstream of brlA.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0005634 nucleus | IBA GO_REF:0000033 | ACCEPT | Summary: Accept: nucleus. Reason: Myb-type factors act in the nucleus, and is_active_in is the appropriate qualifier for a DNA-binding regulator; confirmed directly by the IDA row in this review. |
| GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific | IBA GO_REF:0000033 | ACCEPT | Summary: Accept: DNA-binding transcription factor activity, RNA polymerase II-specific. Reason: Family-level assignment from the Myb/SANT repeat. FlbD's characterised output is induction of brlA, a Pol II gene, so the Pol II-specific TF term fits. |
| GO:0045944 positive regulation of transcription by RNA polymerase II | IBA GO_REF:0000033 | ACCEPT | Summary: Accept: positive regulation of transcription by RNA polymerase II. Reason: The signed activator term, matching FlbD's requirement for brlA induction alongside FlbB rather than any repressive role. |
| GO:0000278 mitotic cell cycle | IBA GO_REF:0000033 | MARK AS OVER ANNOTATED | Summary: Over-annotated: mitotic-cell-cycle role propagated from cell-cycle Myb orthologs. Reason: Propagated by phylogeny from cell-cycle Myb proteins; FlbD's characterized role is developmental regulation, not the mitotic cell cycle. |
| GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding | IBA GO_REF:0000033 | ACCEPT | Summary: Accept: RNA polymerase II cis-regulatory region sequence-specific DNA binding. Reason: The sequence-specific binding facet of the same TF activity. Myb repeats are sequence-specific DNA-binding modules, so this term is preferable to bare DNA binding. |
| GO:0003006 developmental process involved in reproduction | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Generic reproductive-development term (electronic). Reason: Broad electronic term; loosely consistent but uninformative. |
| GO:0003677 DNA binding | IEA GO_REF:0000104 | ACCEPT | Summary: Accept: DNA binding. Reason: Unsigned keyword-level parent of the sequence-specific binding terms above; retained, but uninformative on its own. |
| GO:0005634 nucleus | IEA GO_REF:0000120 | ACCEPT | Summary: Accept: nucleus. Reason: Nuclear location predicted from the Myb domain signature, and independently confirmed by direct localisation in this review. |
| GO:0006351 DNA-templated transcription | IEA GO_REF:0000104 | KEEP AS NON CORE | Summary: Borderline: FlbD regulates transcription rather than catalyzing it. Reason: 'DNA-templated transcription' implies performing transcription; for a TF the regulation terms are more accurate. Kept non-core. |
| GO:0006355 regulation of DNA-templated transcription | IEA GO_REF:0000120 | ACCEPT | Summary: Accept: regulation of DNA-templated transcription. Reason: Generic regulation parent. The Pol II-specific and conidiation terms elsewhere in this review carry the specificity. |
| GO:0009653 anatomical structure morphogenesis | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Generic morphogenesis term (electronic). Reason: Loosely consistent with conidiophore morphogenesis but uninformative. |
| GO:0009791 post-embryonic development | IEA GO_REF:0000117 | MARK AS OVER ANNOTATED | Summary: Over-annotated: animal/plant 'post-embryonic development' term. Reason: ARBA propagation from multicellular animal/plant Myb orthologs; not applicable to fungal conidiation. |
| GO:0009888 tissue development | IEA GO_REF:0000117 | MARK AS OVER ANNOTATED | Summary: Over-annotated: 'tissue development' is an animal/plant multicellular term. Reason: Filamentous fungi do not form tissues in this sense; ARBA over-propagation. |
| GO:0030154 cell differentiation | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Cell differentiation (generic). Reason: Conidiation is a form of cell differentiation, but the specific conidiation terms are preferred; kept non-core. |
| GO:0032875 regulation of DNA endoreduplication | IEA GO_REF:0000117 | MARK AS OVER ANNOTATED | Summary: Over-annotated: DNA-endoreduplication regulation from plant/animal Myb. Reason: No evidence FlbD regulates endoreduplication in A. nidulans; ARBA over-propagation. |
| GO:0033993 response to lipid | IEA GO_REF:0000117 | MARK AS OVER ANNOTATED | Summary: Over-annotated: 'response to lipid' not established for FlbD. Reason: ARBA electronic propagation without experimental support in A. nidulans. |
| GO:0043565 sequence-specific DNA binding | IEA GO_REF:0000117 | ACCEPT | Summary: Sequence-specific DNA binding (Myb). Reason: Consistent with FlbD's Myb DNA-binding domain. |
| GO:0048582 positive regulation of post-embryonic development | IEA GO_REF:0000117 | MARK AS OVER ANNOTATED | Summary: Over-annotated: positive regulation of post-embryonic development (animal/plant). Reason: ARBA propagation from multicellular orthologs; inapplicable to fungi. |
| GO:0048731 system development | IEA GO_REF:0000117 | MARK AS OVER ANNOTATED | Summary: Over-annotated: 'system development' is an animal/plant term. Reason: ARBA over-propagation; not applicable to a fungus. |
| GO:0050891 multicellular organismal-level water homeostasis | IEA GO_REF:0000117 | REMOVE | Summary: Taxon-inappropriate: plant water-homeostasis term. Reason: 'Multicellular organismal-level water homeostasis' is a plant/animal physiology term with no basis in Aspergillus; a clear ARBA mis-propagation. |
| GO:0097305 response to alcohol | IEA GO_REF:0000117 | MARK AS OVER ANNOTATED | Summary: Over-annotated: 'response to alcohol' not established for FlbD. Reason: ARBA electronic propagation without experimental support. |
| GO:1901002 positive regulation of response to salt stress | IEA GO_REF:0000117 | REMOVE | Summary: Taxon-inappropriate: plant salt-stress-response regulation. Reason: Propagated from plant Myb stress-response regulators; no basis in A. nidulans FlbD. |
| GO:1902584 positive regulation of response to water deprivation | IEA GO_REF:0000117 | REMOVE | Summary: Taxon-inappropriate: plant water-deprivation-response regulation. Reason: Propagated from plant Myb drought-response regulators; inapplicable to a fungus. |
| GO:1902806 regulation of cell cycle G1/S phase transition | IEA GO_REF:0000117 | MARK AS OVER ANNOTATED | Summary: Over-annotated: G1/S cell-cycle regulation from mammalian Myb. Reason: Propagated from mammalian cell-cycle Myb proteins; not FlbD's characterized role. |
| GO:2000037 regulation of stomatal complex patterning | IEA GO_REF:0000117 | REMOVE | Summary: Taxon-inappropriate: plant-specific stomatal-patterning term. Reason: Regulation of stomatal complex patterning applies only to plants; A. nidulans has no stomata. A clear taxon-violating over-propagation. |
| GO:0006995 cellular response to nitrogen starvation | IMP PMID:22798393 FlbD, a Myb transcription factor of Aspergillus nidulans, is... | KEEP AS NON CORE | Summary: Nitrogen starvation is an upstream cue for FlbD-dependent development. Reason: Nitrogen limitation induces development; a regulatory input, kept non-core. |
| GO:0070791 cleistothecium development | IMP PMID:22798393 FlbD, a Myb transcription factor of Aspergillus nidulans, is... | ACCEPT | Summary: Core process. FlbD is required for cleistothecium (sexual) development. Reason: FlbD is uniquely required for both asexual and sexual differentiation, including cleistothecium development. Supporting Evidence: PMID:22798393 both ΞflbD and flbD(R47P) mutants are unable to develop the peridium, a specialized external tissue that differentiates during fruiting body formation and ends up surrounding the sexual spores PMID:22798393 flbB to -D encode transcription factors needed for proper expression of the brlA gene, which is essential for asexual development. |
| GO:0075307 positive regulation of conidium formation | IMP PMID:22798393 FlbD, a Myb transcription factor of Aspergillus nidulans, is... | ACCEPT | Summary: Core process. FlbD positively regulates conidium formation. Reason: FlbD is an upstream activator positively regulating conidiation. Supporting Evidence: PMID:22798393 both ΞflbD and flbD(R47P) mutants are unable to develop the peridium, a specialized external tissue that differentiates during fruiting body formation and ends up surrounding the sexual spores PMID:22798393 flbB to -D encode transcription factors needed for proper expression of the brlA gene, which is essential for asexual development. |
| GO:0048315 conidium formation | IMP PMID:7883170 flbD encodes a Myb-like DNA-binding protein that coordinates... | ACCEPT | Summary: Core process. FlbD coordinates initiation of conidiophore development. Reason: flbD encodes a Myb-like protein coordinating initiation of conidiophore development. Supporting Evidence: PMID:7883170 forced overexpression of flbD in submerged hyphae caused inappropriate activation of brlA expression and resulted in production of complex conidiophores that produced all of the distinct cell types observed in wild-type conidiophores including viable spores PMID:7883170 The deduced FlbD protein sequence predicts a 314-amino-acid polypeptide with significant identity at its amino terminus to the DNA-binding domain of the Myb family of transcription factors indicating that FlbD probably functions as a sequence-specific transcriptional activator. |
| GO:0005634 nucleus | IDA PMID:20132447 The concerted action of bZip and cMyb transcription factors ... | ACCEPT | Summary: Accept: nucleus. Reason: Direct nuclear localisation in the study showing concerted FlbB/FlbD action on brlA. This row is what grounds the phylogenetic and electronic location calls above. |
| GO:0075307 positive regulation of conidium formation | IMP PMID:7883170 flbD encodes a Myb-like DNA-binding protein that coordinates... | ACCEPT | Summary: Core process. FlbD positively regulates conidium formation. Reason: Consistent with FlbD as an upstream positive regulator of conidiation. Supporting Evidence: PMID:7883170 forced overexpression of flbD in submerged hyphae caused inappropriate activation of brlA expression and resulted in production of complex conidiophores that produced all of the distinct cell types observed in wild-type conidiophores including viable spores PMID:7883170 The deduced FlbD protein sequence predicts a 314-amino-acid polypeptide with significant identity at its amino terminus to the DNA-binding domain of the Myb family of transcription factors indicating that FlbD probably functions as a sequence-specific transcriptional activator. |
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