vosA ("viability of spores A") is a velvet-family regulatory protein that governs spore maturation and dormancy in Aspergillus nidulans. Expressed specifically during formation of both asexual (conidia) and sexual (ascospores) spores, VosA couples sporogenesis to trehalose biogenesis and, together with VelB, forms an NF-kB-like DNA-binding regulatory complex that represses cell-wall (beta-glucan) biosynthetic genes in spores and confers spore viability, trehalose accumulation and long-term stress resistance. VosA also feeds back to restrain premature germination and asexual development.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0005634 nucleus | IBA GO_REF:0000033 | ACCEPT | Summary: Nuclear velvet regulator. Reason: VosA is a nuclear DNA-binding regulatory protein; consistent with IDA nucleus annotations. |
| GO:0005992 trehalose biosynthetic process | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: VosA regulates trehalose biogenesis in spores (regulatory, not enzymatic). Reason: VosA couples sporogenesis to trehalose biosynthesis by regulating trehalose-pathway genes; it is a regulator, not a biosynthetic enzyme, so this is a non-core regulatory role. Supporting Evidence: PMID:17912349 we identify the novel regulator VosA that couples the formation of spores and focal trehalose biogenesis in the model fungus Aspergillus nidulans |
| GO:0030435 sporulation resulting in formation of a cellular spore | IBA GO_REF:0000033 | ACCEPT | Summary: Core process. VosA functions in cellular-spore formation/maturation. Reason: VosA is required for proper spore maturation and viability of both conidia and ascospores. |
| GO:0005634 nucleus | IEA GO_REF:0000044 | ACCEPT | Summary: Nuclear localization (UniProt mapping). Reason: Consistent with the experimentally determined nuclear localization. |
| GO:0005515 protein binding | IPI PMID:24391470 The velvet family of fungal regulators contains a DNA-bindin... | KEEP AS NON CORE | Summary: Protein binding (uninformative MF) reflecting velvet-complex interaction. Reason: This IPI captures VosA's physical interaction within the velvet regulatory complex; 'protein binding' is uninformative as a molecular function, so kept as non-core. |
| GO:0042802 identical protein binding | IPI PMID:24391470 The velvet family of fungal regulators contains a DNA-bindin... | KEEP AS NON CORE | Summary: Identical protein binding (velvet dimerization). Reason: Reflects velvet-family homo/hetero-dimerization; retained as non-core supporting evidence for complex formation. |
| GO:0048315 conidium formation | IMP PMID:25960370 Velvet-mediated repression of Ξ²-glucan synthesis in Aspergil... | ACCEPT | Summary: Core process. VosA regulates conidium formation/maturation. Reason: VosA (with VelB) represses spore cell-wall biosynthetic genes and is required for proper conidial maturation. Supporting Evidence: PMID:25960370 turned off by the NF-kB like fungal regulators VosA and VelB in Aspergillus nidulans |
| GO:0005634 nucleus | IDA PMID:21152013 LaeA control of velvet family regulatory proteins for light-... | ACCEPT | Summary: Nuclear localization (direct). Reason: Direct assay places VosA in the nucleus, consistent with its DNA-binding regulatory role. |
| GO:0005992 trehalose biosynthetic process | IMP PMID:21152013 LaeA control of velvet family regulatory proteins for light-... | KEEP AS NON CORE | Summary: VosA regulates trehalose biosynthesis (direct, regulatory). Reason: Direct genetic evidence that VosA controls trehalose biogenesis; a regulatory rather than enzymatic role. Supporting Evidence: PMID:17912349 The vosA gene is expressed specifically during the formation of both sexual and asexual spores (conidia) |
| GO:0005634 nucleus | IDA PMID:17912349 A novel regulator couples sporogenesis and trehalose biogene... | ACCEPT | Summary: Nuclear localization (direct). Reason: Direct assay; consistent with the founding VosA characterization. |
| GO:0030435 sporulation resulting in formation of a cellular spore | IMP PMID:17912349 A novel regulator couples sporogenesis and trehalose biogene... | ACCEPT | Summary: Core process. VosA governs cellular-spore formation. Reason: Gain-of-function and deletion analyses establish VosA as a regulator of spore formation and viability. Supporting Evidence: PMID:17912349 we identify the novel regulator VosA that couples the formation of spores and focal trehalose biogenesis in the model fungus Aspergillus nidulans |
| GO:0048315 conidium formation | IMP PMID:17912349 A novel regulator couples sporogenesis and trehalose biogene... | ACCEPT | Summary: Core process. VosA is required for conidium formation/maturation. Reason: VosA is expressed during conidiogenesis and required for conidial maturation and viability. Supporting Evidence: PMID:17912349 The vosA gene is expressed specifically during the formation of both sexual and asexual spores (conidia) |
Loading supporting contentβ¦
Download this section (compressed HTML)Loading supporting contentβ¦
Download this section (compressed HTML)Loading supporting contentβ¦
Download this section (compressed HTML)