fosA5

UniProt ID: A0A346FWD3
Organism: Enterobacter cloacae
Review Status: DRAFT
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Gene Description

fosA5 encodes a FosA-family fosfomycin resistance enzyme in Enterobacter cloacae. UniProt/CARD identify this entry as a glutathione-dependent fosfomycin-modifying enzyme; the defensible GO molecular function is glutathione transferase activity for FosA-family members. UniProt names the protein 'Fosfomycin resistance glutathione transferase FosA5' (accession A0A346FWD3).

Existing Annotations Review

GO Term Evidence Action Reason
GO:0004364 glutathione transferase activity
RCA
file:projects/ANTIMICROBIAL_RESISTANCE/aro2go.sssom.yaml 		NEW
Summary: NEW candidate annotation from the curated ARO->GO mapping: glutathione transferase activity.
Reason: The UniProt record cross-references a CARD/ARO AMR determinant, and the curated ARO->GO mapping projects the family-specific molecular function glutathione transferase activity. This focused draft accepts the candidate as a curator lead for this AMR determinant.
Supporting Evidence:
file:projects/ANTIMICROBIAL_RESISTANCE/data/candidate_new_annotations.tsv
A0A346FWD3 ARO:3003209 ARO:3003209 exact skos:relatedMatch GO:0004364 glutathione transferase activity
file:genes/ENTCL/fosA5/fosA5-uniprot.txt
DR CARD; ARO:3003209; fosA5; ARO:0001004; antibiotic inactivation.
GO:0046677 response to antibiotic
RCA
file:projects/ANTIMICROBIAL_RESISTANCE/aro2go.sssom.yaml 		NEW
Summary: NEW process annotation: fosA5 is an AMR determinant involved in antibiotic response/resistance.
Reason: CARD/ARO identity places this gene in an antimicrobial-resistance determinant family; response to antibiotic is the appropriate high-level GO biological process for the resistance role.
Supporting Evidence:
file:genes/ENTCL/fosA5/fosA5-uniprot.txt
DR CARD; ARO:3003209; fosA5; ARO:0001004; antibiotic inactivation.

Core Functions

fosA5 inactivates fosfomycin through glutathione-dependent modification, contributing to antibiotic resistance.

Molecular Function:
glutathione transferase activity
Directly Involved In:
response to antibiotic
Supporting Evidence:
  • file:genes/ENTCL/fosA5/fosA5-uniprot.txt
    DR CARD; ARO:3003209; fosA5; ARO:0001004; antibiotic inactivation.

References

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Suggested Questions for Experts

Q: Is the ARO-derived glutathione transferase activity annotation sufficiently specific for fosA5, or is a narrower substrate/site-specific GO term warranted?

Suggested Experiments

Experiment: Biochemically assay purified fosA5 against representative antibiotic substrates for the inferred AMR family and measure loss of drug activity or target modification.

Type: in vitro enzyme assay

📚 Additional Documentation

Notes

(fosA5-notes.md)

Notes: fosA5

Focused AMR batch review. UniProt accession: A0A346FWD3. Source organism: Enterobacter cloacae.

  • UniProt/CARD provenance: DR CARD; ARO:3003209; fosA5; ARO:0001004; antibiotic inactivation.
  • ARO-to-GO candidate: GO:0004364 (glutathione transferase activity) from projects/ANTIMICROBIAL_RESISTANCE/data/candidate_new_annotations.tsv and projects/ANTIMICROBIAL_RESISTANCE/aro2go.sssom.yaml.
  • This is a DRAFT focused review intended to cover the AMR annotation gap; it has not had a gene-specific deep-research pass.

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