id: A0A9L0J5U7
gene_symbol: ALKAL2
taxon:
  id: NCBITaxon:9793
  label: Equus asinus
status: COMPLETE
description: Assessment of the single ProtNLM2 GO prediction for donkey ALKAL2 (A0A9L0J5U7), judged against
  the donkey GOA record (extracellular region, plasma membrane, receptor signaling protein tyrosine kinase activator
  activity, receptor tyrosine kinase binding, ERK1/2 and ERK5 cascade regulation), the human ortholog Q6UX46
  and its characterization, and a donkey-human alignment (86% identity, invariant augmentor domain). The prediction,
  extracellular region, is correct and already in donkey GOA (CNN). The frozen ProtNLM2 output contains no
  non-GO rows (function text or subcellular location) for this accession.
source_documents:
- projects/PROTNLM_EVALUATION/donkey40/donkey40-predictions.tsv
- genes/EQUAS/ALKAL2/ALKAL2-goa.tsv
- genes/EQUAS/ALKAL2/ALKAL2-bioinformatics/ALKAL2-human-Q6UX46-uniprot.txt
- genes/EQUAS/ALKAL2/ALKAL2-bioinformatics/RESULTS.md
predictions:
- source_method: ProtNLM2
  source_version: UniProt API snapshot 2026-10-14
  predicted_term:
    id: GO:0005576
    label: extracellular region
  predicted_term_type: GO_CC
  review:
    assessment: CNN
    confidence_score: 2
    summary: ALKAL2 is a secreted cytokine; the human ortholog is experimentally secreted and active when supplied
      as conditioned medium, and the donkey protein has a predicted N-terminal signal peptide and is 86% identical
      to human across its full length. The exact term is already in donkey GOA (UniProt subcellular-location
      mapping, accepted as a core location in the gene review), so the prediction is correct but not novel. Human ALKAL2 also associates with the cell membrane
      once bound to ALK, but extracellular region is the appropriate primary location for the ligand.
    supported_by:
    - reference_id: file:EQUAS/ALKAL2/ALKAL2-bioinformatics/ALKAL2-human-Q6UX46-uniprot.txt
      supporting_text: 'SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:30061385}.'
    - reference_id: PMID:26418745
      supporting_text: Addition of conditioned medium from cells expressing FAM150A or FAM150B led to strong
        activation of ALK in receiving cells that expressed ALK
references:
- id: file:EQUAS/ALKAL2/ALKAL2-goa.tsv
  title: Donkey ALKAL2 GOA annotations
  findings: []
- id: file:EQUAS/ALKAL2/ALKAL2-bioinformatics/ALKAL2-human-Q6UX46-uniprot.txt
  title: UniProt Swiss-Prot record for human ALKAL2 (Q6UX46)
  findings: []
- id: file:EQUAS/ALKAL2/ALKAL2-bioinformatics/RESULTS.md
  title: 'ALKAL2: donkey-human sequence comparison'
  findings: []
- id: PMID:26418745
  title: FAM150A and FAM150B are activating ligands for anaplastic lymphoma kinase.
  findings: []