id: A0A9L0IHI0
gene_symbol: ATG3
taxon:
  id: NCBITaxon:9793
  label: Equus asinus
status: COMPLETE
description: >-
  Assessment of the single ProtNLM2 GO prediction for donkey ATG3 (A0A9L0IHI0, DONKEY40 cohort), using
  the reviewed human ortholog Q9NT62, human ATG3 literature, and a donkey-human alignment (98.41% identity,
  catalytic cysteine conserved, 28-residue insertion in the donkey model). The prediction, cytoplasm, is
  correct but less precise than the cytosol and phagophore assembly site annotations already in donkey GOA
  from TreeGrafter (LSP); the ProtNLM2 evidence record cites phagophore assembly site. ProtNLM2 emitted no
  function text or subcellular-location statement for this accession in the snapshot.
source_documents:
- projects/PROTNLM_EVALUATION/donkey40/donkey40-predictions.tsv
- projects/PROTNLM_EVALUATION/donkey40/donkey40-evidence.tsv
- genes/EQUAS/ATG3/ATG3-goa.tsv
- genes/EQUAS/ATG3/ATG3-bioinformatics/RESULTS.md
- genes/EQUAS/ATG3/ATG3-bioinformatics/human-Q9NT62-uniprot.txt
predictions:
- source_method: ProtNLM2
  source_version: UniProt API snapshot 2026-10-14
  predicted_term:
    id: GO:0005737
    label: cytoplasm
  predicted_term_type: GO_CC
  review:
    assessment: LSP
    confidence_score: 2
    summary: Human ATG3 is experimentally cytoplasmic and acts as a soluble E2 that is recruited to phagophore
      membranes; the donkey protein is 98.4% identical with the catalytic cysteine and membrane-sensing
      N-terminus conserved, so cytoplasm is correct. Donkey GOA already contains cytosol and phagophore assembly
      site (TreeGrafter), both part of cytoplasm in GO, so the prediction is less precise than existing
      annotation. Training-set membership is unknown.
    supported_by:
    - reference_id: file:EQUAS/ATG3/ATG3-bioinformatics/human-Q9NT62-uniprot.txt
      supporting_text: 'CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:11825910}.'
    - reference_id: file:EQUAS/ATG3/ATG3-bioinformatics/RESULTS.md
      supporting_text: the catalytic Cys-264 (glycyl thioester intermediate) aligns to donkey Cys-292
references:
- id: file:EQUAS/ATG3/ATG3-bioinformatics/RESULTS.md
  title: 'ATG3: donkey-human sequence comparison'
  findings: []
- id: file:EQUAS/ATG3/ATG3-bioinformatics/human-Q9NT62-uniprot.txt
  title: UniProtKB/Swiss-Prot record Q9NT62 (human ATG3), downloaded 2026-10-14
  findings: []