ID   A0A9L0IIU3_EQUAS        Unreviewed;       458 AA.
AC   A0A9L0IIU3;
DT   13-SEP-2023, integrated into UniProtKB/TrEMBL.
DT   13-SEP-2023, sequence version 1.
DT   02-SEP-2026, entry version 14.
DE   RecName: Full=Cysteine protease {ECO:0000256|RuleBase:RU363115};
DE            EC=3.4.22.- {ECO:0000256|RuleBase:RU363115};
GN   Name=ATG4C {ECO:0000313|Ensembl:ENSEASP00005037695.1};
OS   Equus asinus (Donkey) (Equus africanus asinus).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Perissodactyla; Equidae; Equus.
OX   NCBI_TaxID=9793 {ECO:0000313|Ensembl:ENSEASP00005037695.1, ECO:0000313|Proteomes:UP000694387};
RN   [1] {ECO:0000313|Ensembl:ENSEASP00005037695.1, ECO:0000313|Proteomes:UP000694387}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=33293529;
RA   Wang, C., Li, H., Guo, Y., Huang, J., Sun, Y., Min, J., Wang, J., Fang, X.,
RA   Zhao, Z., Wang, S., Zhang, Y., Liu, Q., Jiang, Q., Wang, X., Guo, Y., Yang,
RA   C., Wang, Y., Tian, F., Zhuang, G., Fan, Y., Gao, Q., Li, Y., Ju, Z., Li,
RA   J., Li, R., Hou, M., Yang, G., Liu, G., Liu, W., Guo, J., Pan, S., Fan, G.,
RA   Zhang, W., Zhang, R., Yu, J., Zhang, X., Yin, Q., Ji, C., Jin, Y., Yue, G.,
RA   Liu, M., Xu, J., Liu, S., Jordana, J., Noce, A., Amills, M., Wu, D.D., Li,
RA   S., Zhou, X. and Zhong, J.;
RT   "Donkey genomes provide new insights into domestication and selection for
RT   coat color.";
RL   Nat. Commun. 11:6014-6014(2020).
RN   [2] {ECO:0000313|Ensembl:ENSEASP00005037695.1}
RP   IDENTIFICATION.
RG   Ensembl;
RL   Submitted (JAN-2026) to UniProtKB.
CC   -!- FUNCTION: Cysteine protease that plays a key role in autophagy by
CC       mediating both proteolytic activation and delipidation of ATG8 family
CC       proteins. {ECO:0000256|RuleBase:RU363115}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=[protein]-C-terminal L-amino acid-glycyl-
CC         phosphatidylethanolamide + H2O = [protein]-C-terminal L-amino acid-
CC         glycine + a 1,2-diacyl-sn-glycero-3-phosphoethanolamine;
CC         Xref=Rhea:RHEA:67548, Rhea:RHEA-COMP:17323, Rhea:RHEA-COMP:17324,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:64612, ChEBI:CHEBI:172940,
CC         ChEBI:CHEBI:172941; Evidence={ECO:0000256|ARBA:ARBA00029362};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:67549;
CC         Evidence={ECO:0000256|ARBA:ARBA00029362};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496,
CC       ECO:0000256|RuleBase:RU363115}.
CC   -!- SIMILARITY: Belongs to the peptidase C54 family.
CC       {ECO:0000256|ARBA:ARBA00010958, ECO:0000256|RuleBase:RU363115}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   RefSeq; XP_014710846.1; XM_014855360.3.
DR   RefSeq; XP_070342532.1; XM_070486431.1.
DR   RefSeq; XP_070342533.1; XM_070486432.1.
DR   RefSeq; XP_070342534.1; XM_070486433.1.
DR   RefSeq; XP_070342535.1; XM_070486434.1.
DR   AlphaFoldDB; A0A9L0IIU3; -.
DR   Ensembl; ENSEAST00005066709.1; ENSEASP00005037695.1; ENSEASG00005008203.2.
DR   GeneID; 106840155; -.
DR   KEGG; eai:106840155; -.
DR   CTD; 84938; -.
DR   GeneTree; ENSGT00530000063000; -.
DR   OMA; KMAGDWY; -.
DR   Proteomes; UP000694387; Chromosome 16.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004197; F:cysteine-type endopeptidase activity; IEA:TreeGrafter.
DR   GO; GO:0019786; F:protein-phosphatidylethanolamide deconjugating activity; IEA:InterPro.
DR   GO; GO:0035973; P:aggrephagy; IEA:TreeGrafter.
DR   GO; GO:0000045; P:autophagosome assembly; IEA:TreeGrafter.
DR   GO; GO:0000423; P:mitophagy; IEA:TreeGrafter.
DR   GO; GO:0034727; P:piecemeal microautophagy of the nucleus; IEA:TreeGrafter.
DR   GO; GO:0016485; P:protein processing; IEA:TreeGrafter.
DR   InterPro; IPR046793; ATG4_LIR.
DR   InterPro; IPR038765; Papain-like_cys_pep_sf.
DR   InterPro; IPR005078; Peptidase_C54.
DR   InterPro; IPR046792; Peptidase_C54_cat.
DR   PANTHER; PTHR22624; CYSTEINE PROTEASE ATG4; 1.
DR   PANTHER; PTHR22624:SF38; CYSTEINE PROTEASE ATG4C; 1.
DR   Pfam; PF20166; ATG4_LIR; 1.
DR   Pfam; PF03416; Peptidase_C54; 1.
DR   SUPFAM; SSF54001; Cysteine proteinases; 1.
PE   3: Inferred from homology;
KW   Autophagy {ECO:0000256|ARBA:ARBA00023006, ECO:0000256|RuleBase:RU363115};
KW   Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|RuleBase:RU363115};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU363115};
KW   Protease {ECO:0000256|ARBA:ARBA00022670, ECO:0000256|RuleBase:RU363115};
KW   Protein transport {ECO:0000256|ARBA:ARBA00022927,
KW   ECO:0000256|RuleBase:RU363115};
KW   Reference proteome {ECO:0000313|Proteomes:UP000694387};
KW   Thiol protease {ECO:0000256|ARBA:ARBA00022807};
KW   Transport {ECO:0000256|ARBA:ARBA00022448};
KW   Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786}.
FT   DOMAIN          77..398
FT                   /note="Peptidase C54 catalytic"
FT                   /evidence="ECO:0000259|Pfam:PF03416"
SQ   SEQUENCE   458 AA;  52587 MW;  8576BEA7B7F130B8 CRC64;
     MEATGTDEVD KLKTKFISAW NNMKYSWVLK TKTYFSRNSP VLLLGKCYHF KYEDENKLLP
     ARSGCTIEDH IIAGNVEEFR KDFTSRIWLT YREEFPQIEG STLTTDCGWG CTLRTGQMLL
     AQGLILHFLG RAWTWPDALN IENSDFESWT SNTVKKFTAS FEASLSEERE LKTPTISLKE
     TIGRYSDDHE MRNEIYHRKI ISWFGDSPLA LFGLHQLIEY GKKSGKKAGD WYGPAVVAHI
     LRKAVEEARH PDLQGITIYV AQDCTVYSSD VIDKQCASMA SDHADDKAVI ILVPVRLGGE
     RTNTDYLDFV KGILSLEYCV GIIGGKPKQS YYFAGFQDDS LIYMDPHYCQ SFVDVSIKDF
     PLETFHCPSP KKMSFRKMDP SCTIGFYCRN VQDFKRASEE ITKMLKISSK EKYPLFTFVN
     GHSRDYDFTS TAAKEDDLFS EDEKKRLKRF STEEFVLL
//