id: A0A8C4MED2
gene_symbol: DUSP5
taxon:
  id: NCBITaxon:9793
  label: Equus asinus
status: COMPLETE
description: Assessment of the single ProtNLM2 GO prediction for donkey DUSP5 (A0A8C4MED2), a nuclear
  dual-specificity MAP kinase phosphatase 94.8% identical to human DUSP5. The prediction, myosin phosphatase
  activity, is refuted (NPI). ProtNLM2 emitted no function text or subcellular-location output for this
  accession in the frozen snapshot.
source_documents:
- projects/PROTNLM_EVALUATION/donkey40/donkey40-predictions.tsv
- genes/EQUAS/DUSP5/DUSP5-goa.tsv
- genes/EQUAS/DUSP5/DUSP5-bioinformatics/human-Q16690-uniprot.txt
- genes/EQUAS/DUSP5/DUSP5-bioinformatics/RESULTS.md
predictions:
- source_method: ProtNLM2
  source_version: UniProt API snapshot 2026-10-14
  predicted_term:
    id: GO:0017018
    label: myosin phosphatase activity
  predicted_term_type: GO_MF
  review:
    assessment: NPI
    confidence_score: 0
    summary: GO:0017018 is defined as catalysis of phosphomyosin + H2O = myosin + phosphate, an activity
      carried out by the PP1c-MYPT1 myosin phosphatase holoenzyme of the PPP family. DUSP5 is a cysteine-based
      dual-specificity phosphatase of the PTP superfamily whose characterized substrate is ERK; human
      DUSP5 inactivates ERK2 but not JNK or p38, and its activity is highest toward ERK1. The donkey protein
      conserves the catalytic Cys263 and is 94.8% identical to human over its full length, so it is expected
      to share this ERK-directed specificity. No evidence links DUSP5 to myosin dephosphorylation, and
      the donkey GOA already records the correct MAP kinase tyrosine/serine/threonine phosphatase activity.
      The one study a PubMed search finds linking DUSP5 to myosin places it upstream, reporting that DUSP5 lowers myosin IIB activation indirectly
      by inhibiting ERK, which acts through MLCK, rather than by dephosphorylating myosin itself. The prediction
      assigns a substrate-specific phosphatase activity that belongs to an unrelated enzyme family (PPP-type
      myosin phosphatase), not to a paralog. No error-type value fits a training-data origin that cannot be
      shown here, so none is set.
    supported_by:
    - reference_id: file:EQUAS/DUSP5/DUSP5-bioinformatics/human-Q16690-uniprot.txt
      supporting_text: highest relative activity is toward ERK1.
    - reference_id: PMID:15713638
      supporting_text: This binding determines the substrate specificity of DUSP5 in vivo, as it inactivates
        ERK2 but not Jun N-terminal protein kinase or p38 MAP kinase.
    - reference_id: file:EQUAS/DUSP5/DUSP5-bioinformatics/RESULTS.md
      supporting_text: 'ACT_SITE Phosphocysteine intermediate: human [263] C -> donkey [263] C'
    - reference_id: PMID:37149044
      supporting_text: suppressing F-actin rearrangement via negatively regulating the ERK-MLCK-Myosin IIB
        signaling axis
references:
- id: PMID:15713638
  title: Specific inactivation and nuclear anchoring of extracellular signal-regulated kinase 2 by the
    inducible dual-specificity protein phosphatase DUSP5.
  findings: []
- id: PMID:37149044
  title: Host factor DUSP5 potently inhibits dengue virus infection by modulating cytoskeleton rearrangement.
  findings: []
- id: file:EQUAS/DUSP5/DUSP5-bioinformatics/human-Q16690-uniprot.txt
  title: UniProt record for human DUSP5 (Q16690)
  findings: []
- id: file:EQUAS/DUSP5/DUSP5-bioinformatics/RESULTS.md
  title: 'DUSP5: donkey-human sequence comparison'
  findings: []