ID   A0A9L0KBR4_EQUAS        Unreviewed;       681 AA.
AC   A0A9L0KBR4;
DT   13-SEP-2023, integrated into UniProtKB/TrEMBL.
DT   13-SEP-2023, sequence version 1.
DT   10-JUN-2026, entry version 14.
DE   SubName: Full=EPS8 signaling adaptor L2 {ECO:0000313|Ensembl:ENSEASP00005062258.1};
GN   Name=EPS8L2 {ECO:0000313|Ensembl:ENSEASP00005062258.1};
OS   Equus asinus (Donkey) (Equus africanus asinus).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Perissodactyla; Equidae; Equus.
OX   NCBI_TaxID=9793 {ECO:0000313|Ensembl:ENSEASP00005062258.1, ECO:0000313|Proteomes:UP000694387};
RN   [1] {ECO:0000313|Ensembl:ENSEASP00005062258.1, ECO:0000313|Proteomes:UP000694387}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=33293529;
RA   Wang, C., Li, H., Guo, Y., Huang, J., Sun, Y., Min, J., Wang, J., Fang, X.,
RA   Zhao, Z., Wang, S., Zhang, Y., Liu, Q., Jiang, Q., Wang, X., Guo, Y., Yang,
RA   C., Wang, Y., Tian, F., Zhuang, G., Fan, Y., Gao, Q., Li, Y., Ju, Z., Li,
RA   J., Li, R., Hou, M., Yang, G., Liu, G., Liu, W., Guo, J., Pan, S., Fan, G.,
RA   Zhang, W., Zhang, R., Yu, J., Zhang, X., Yin, Q., Ji, C., Jin, Y., Yue, G.,
RA   Liu, M., Xu, J., Liu, S., Jordana, J., Noce, A., Amills, M., Wu, D.D., Li,
RA   S., Zhou, X. and Zhong, J.;
RT   "Donkey genomes provide new insights into domestication and selection for
RT   coat color.";
RL   Nat. Commun. 11:6014-6014(2020).
RN   [2] {ECO:0000313|Ensembl:ENSEASP00005062258.1}
RP   IDENTIFICATION.
RG   Ensembl;
RL   Submitted (JAN-2026) to UniProtKB.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC   -!- SIMILARITY: Belongs to the EPS8 family.
CC       {ECO:0000256|ARBA:ARBA00006197}.
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DR   AlphaFoldDB; A0A9L0KBR4; -.
DR   Ensembl; ENSEAST00005047095.1; ENSEASP00005062258.1; ENSEASG00005007425.2.
DR   GeneTree; ENSGT00940000160990; -.
DR   Proteomes; UP000694387; Chromosome 17.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0032587; C:ruffle membrane; IEA:TreeGrafter.
DR   GO; GO:0031982; C:vesicle; IEA:TreeGrafter.
DR   GO; GO:0003779; F:actin binding; IEA:TreeGrafter.
DR   GO; GO:1900029; P:positive regulation of ruffle assembly; IEA:TreeGrafter.
DR   GO; GO:0035023; P:regulation of Rho protein signal transduction; IEA:TreeGrafter.
DR   GO; GO:0007266; P:Rho protein signal transduction; IEA:TreeGrafter.
DR   CDD; cd01210; PTB_EPS8; 1.
DR   CDD; cd09540; SAM_EPS8-like; 1.
DR   FunFam; 1.10.150.50:FF:000023; Epidermal growth factor receptor kinase substrate 8; 1.
DR   FunFam; 2.30.29.30:FF:000218; Epidermal growth factor receptor kinase substrate 8-like 2; 1.
DR   Gene3D; 2.30.29.30; Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB); 1.
DR   Gene3D; 2.30.30.40; SH3 Domains; 1.
DR   Gene3D; 1.10.150.50; Transcription Factor, Ets-1; 1.
DR   InterPro; IPR039801; EPS8-like.
DR   InterPro; IPR055093; EPS8_2nd.
DR   InterPro; IPR033928; EPS8_PTB.
DR   InterPro; IPR011993; PH-like_dom_sf.
DR   InterPro; IPR013625; PTB.
DR   InterPro; IPR006020; PTB/PI_dom.
DR   InterPro; IPR013761; SAM/pointed_sf.
DR   InterPro; IPR041418; SAM_3.
DR   InterPro; IPR036028; SH3-like_dom_sf.
DR   InterPro; IPR001452; SH3_domain.
DR   PANTHER; PTHR12287:SF20; EPIDERMAL GROWTH FACTOR RECEPTOR KINASE SUBSTRATE 8-LIKE PROTEIN 2; 1.
DR   PANTHER; PTHR12287; EPIDERMAL GROWTH FACTOR RECEPTOR KINASE SUBSTRATE EPS8-RELATED PROTEIN; 1.
DR   Pfam; PF22975; EPS8_2nd; 1.
DR   Pfam; PF08416; PTB; 1.
DR   Pfam; PF18016; SAM_3; 1.
DR   Pfam; PF00018; SH3_1; 1.
DR   SMART; SM00462; PTB; 1.
DR   SMART; SM00326; SH3; 1.
DR   SUPFAM; SSF50729; PH domain-like; 1.
DR   SUPFAM; SSF50044; SH3-domain; 1.
DR   PROSITE; PS50002; SH3; 1.
PE   3: Inferred from homology;
KW   Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW   Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW   Reference proteome {ECO:0000313|Proteomes:UP000694387};
KW   SH3 domain {ECO:0000256|ARBA:ARBA00022443, ECO:0000256|PROSITE-
KW   ProRule:PRU00192}.
FT   DOMAIN          459..542
FT                   /note="SH3"
FT                   /evidence="ECO:0000259|PROSITE:PS50002"
FT   REGION          164..195
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          408..444
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        165..174
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   681 AA;  76889 MW;  E8E65BEF3F510366 CRC64;
     MSRAPTDIHQ GREETPLRWG CHLATFIMDK SEAIVSVDDA IRKLVQLSSK EKIWTQEMLL
     QVNDKSLRLL DIESQEELEN FPLPTVRHSQ TVLNQLRYPS VLLLVCQDSE QNKPDIHFFH
     CDEVEAELVH EDIESALADY RLGKKMRPQT LKGHQEKIRQ RLSVLPPPQG PAPIPFQRHG
     GDSPTTKNRV GLPVPLSEPS FLRRESLEEE PRAVLAQKIE KETQILNCAL DDIEWFVARL
     QKAAEAFKQL NQRKKGKKKG KKGPAEGVLT LRARPPSEAE FVDCFQKTKL AINLLAKLQK
     HIQNPSAAEL AHFLFGPLDL IVSTCGGPDI ARSVSSPLLS RDAVGFLRGH LVPKEMALWE
     SLGETWTRPR SEWPREPRVP LYVPKFQSGW EPPLDVLQEA PWEVEGLVSA PSDEPTPGSR
     LSFRSSQKHS LVPEPTSPGD VLPPVSSPHI LRGYEPTPAM AKYVRVLYDF TARNANELSV
     LKDEVLEVLD DSHQWWKLRN RSGQATCQPW RTSPRSMAVG AGQGWAGLKY WGPASPTHKL
     PPSFAGNKNE LIHHMDEVND ELIKKISNIK TQPQRPFRVE RSQPVSLPLT YESGPDEVRA
     WLEAKAFSAR IVENLGILTG PQLFSLNKDE LKKVCGEDGI RVYSQLTVQK AVLEKQQGGS
     ELEELMNKFH SKNQRRMEEE S
//