id: A0A9L0ILJ0
gene_symbol: GPAT2
taxon:
  id: NCBITaxon:9793
  label: Equus asinus
status: COMPLETE
description: >-
  Assessment of the single ProtNLM2 GO prediction for donkey GPAT2 (A0A9L0ILJ0, DONKEY40 cohort). The
  gene review accepts glycerol-3-phosphate O-acyltransferase activity (GO:0004366) as the core activity,
  transferred from the GPAT2 PANTHER subfamily (PTHR12563:SF15) on the basis of measured GPAT activity of
  mouse Gpat2, and the donkey model retains the acyltransferase domain and the HXXXXD catalytic motif.
  A PubMed search found no experimental study of GPAT2 in equids.
  The predicted GO:0008374 O-acyltransferase activity is an obsolete grouping class (replaced by
  GO:0016747); the activity it names is correct but less precise than glycerol-3-phosphate O-acyltransferase
  activity, which donkey GOA already has from TreeGrafter and which the ProtNLM2 evidence record cites (LSP).
  Non-GO ProtNLM2 output for this accession, not scored here: a function statement, "Esterifies acyl-group
  from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipids biosynthesis
  such as triglycerides, phosphatidic acids and lysophosphatidic acids" (supported by a phmmer match to human
  GPAT1/GPAM Q9HCL2 rather than GPAT2; the acyl-ACP wording follows UniProt text, whereas the mammalian
  catalytic-activity lines use acyl-CoA). No subcellular-location statement was emitted.
source_documents:
- projects/PROTNLM_EVALUATION/donkey40/donkey40-predictions.tsv
- projects/PROTNLM_EVALUATION/donkey40/donkey40-evidence.tsv
- genes/EQUAS/GPAT2/GPAT2-goa.tsv
- genes/EQUAS/GPAT2/GPAT2-bioinformatics/RESULTS.md
- genes/EQUAS/GPAT2/GPAT2-bioinformatics/human-Q6NUI2-uniprot.txt
- genes/EQUAS/GPAT2/GPAT2-bioinformatics/RESULTS-rereview.md
- genes/EQUAS/GPAT2/GPAT2-ai-review.yaml
predictions:
- source_method: ProtNLM2
  source_version: UniProt API snapshot 2026-10-14
  predicted_term:
    id: GO:0008374
    label: O-acyltransferase activity
  predicted_term_type: GO_MF
  review:
    assessment: LSP
    confidence_score: 2
    summary: The GPAT2 subfamily esterifies acyl-CoA onto the sn-1 hydroxyl of glycerol 3-phosphate, an
      O-acyl transfer; overexpressed mouse Gpat2 raises GPAT activity with arachidonoyl-CoA, and the donkey
      model conserves the acyltransferase domain and HXXXXD motif, so the predicted activity is correct in
      substance. Scored for this product, A0A9L0ILJ0 (718 aa) keeps the acyltransferase domain (199-333),
      the HXXXXD motif and both transmembrane segments of the full-length 800-residue model A0A8C4KXU5, and
      lacks only the conserved GPAT2 C-terminal region (about 85 residues replaced by a different tail),
      which is not the catalytic region, so the activity does not depend on a feature this product lacks. The term is obsolete in the current GO release (an unnecessary grouping class, replaced
      by GO:0016747, which is also in donkey GOA from InterPro2GO). Donkey GOA already carries the specific
      glycerol-3-phosphate O-acyltransferase activity (TreeGrafter), accepted as core in the gene review,
      so the prediction is less precise than existing annotation. The obsolete identifier is a defect of
      the emitted term, not of the biology. Training-set membership is unknown.
    supported_by:
    - reference_id: PMID:22905194
      supporting_text: GPAT2 overexpression significantly increased both GPAT and AGPAT activities only
        when arachidonoyl-CoA was used as a substrate
    - reference_id: file:EQUAS/GPAT2/GPAT2-bioinformatics/human-Q6NUI2-uniprot.txt
      supporting_text: 'CC   -!- DOMAIN: The HXXXXD motif is essential for acyltransferase activity and'
    - reference_id: file:EQUAS/GPAT2/GPAT2-bioinformatics/RESULTS-rereview.md
      supporting_text: The benchmark A0A9L0ILJ0 matches A0A8C4KXU5 through the acyltransferase domain, the
        HXXXXD motif
    - reference_id: file:EQUAS/GPAT2/GPAT2-bioinformatics/RESULTS.md
      supporting_text: the HXXXXD catalytic motif (human 205-210) is conserved in donkey as H205, K206, L209,
        D210 at the same positions
references:
- id: file:EQUAS/GPAT2/GPAT2-bioinformatics/RESULTS.md
  title: 'GPAT2: donkey-human sequence comparison'
  findings: []
- id: file:EQUAS/GPAT2/GPAT2-bioinformatics/human-Q6NUI2-uniprot.txt
  title: UniProtKB/Swiss-Prot record Q6NUI2 (human GPAT2), downloaded 2026-10-14
  findings: []
- id: file:EQUAS/GPAT2/GPAT2-bioinformatics/RESULTS-rereview.md
  title: 'GPAT2 (donkey): gene-model comparison against mouse Gpat2 (re-review, 2026-10-21)'
  findings: []
- id: PMID:22905194
  title: Glycerol-3-phosphate acyltransferase-2 is expressed in spermatic germ cells and incorporates arachidonic
    acid into triacylglycerols.
  findings: []