== Positive control: human GPC3 P51654 (FT SIGNAL 1..24)
N-terminal 50: MAGTVRTACLVVAMLLSLDFPGQAQPPPPPPDATCHQVRSFFQRLQPGLK
Max Kyte-Doolittle mean, window 7: 3.36 at residues 10..16
Max Kyte-Doolittle mean, window 9: 3.09 at residues 8..16
Max Kyte-Doolittle mean, window 11: 2.80 at residues 8..18
Longest apolar h-region: 8..18 ACLVVAMLLSL (mean KD 2.80)
Signal peptide (n/h/c rule): FOUND, h 7..14, predicted cleavage after 24 (MAGTVRTACLVVAMLLSLDFPGQA)
== Positive control: donkey canonical GPC3 A0A9L0JGR2
N-terminal 50: MAGTVHTACLVVAMLLSLDCPGQAQPPPPPPDATCHQVRSFFQRLQPGLK
Max Kyte-Doolittle mean, window 7: 3.36 at residues 10..16
Max Kyte-Doolittle mean, window 9: 3.09 at residues 8..16
Max Kyte-Doolittle mean, window 11: 2.80 at residues 8..18
Longest apolar h-region: 8..18 ACLVVAMLLSL (mean KD 2.80)
Signal peptide (n/h/c rule): FOUND, h 7..14, predicted cleavage after 24 (MAGTVHTACLVVAMLLSLDCPGQA)
== Query: donkey GPC3 A0A9L0J2B1
N-terminal 50: MSFQFAPGGCYGHARVFDCQMVMMMEARMFIFWPRFWKGTPNKGSDLQVC
Max Kyte-Doolittle mean, window 7: 1.54 at residues 19..25
Max Kyte-Doolittle mean, window 9: 1.38 at residues 16..24
Max Kyte-Doolittle mean, window 11: 1.43 at residues 22..32
Apolar h-region (7-15 residues, start 2..12, <=1 S/T): none
Signal peptide (n/h/c rule): NOT FOUND
== Query: longest apolar run anywhere in A0A9L0J2B1 residues 1..50
Longest apolar run: 5 residues at 21..25 (MVMMM)
Charged/His residues in 1..43: H13 R15 D18 E26 R28 R35 K38 K43