id: A0A9L0K3L6
gene_symbol: KDM4C
taxon:
  id: NCBITaxon:9793
  label: Equus asinus
status: COMPLETE
description: Assessment of the single ProtNLM2 GO prediction for donkey KDM4C (A0A9L0K3L6), a JmjC-domain
  histone demethylase 91% identical to human KDM4C with a fully conserved Fe(II)/2-oxoglutarate active site.
  The prediction histone H3K9me2/H3K9me3 demethylase activity (GO:0140684) is correct and already present
  in donkey GOA (CNN). ProtNLM2 also emitted the function text "Histone demethylase that specifically demethylates
  'Lys-9' of histone H3, thereby playing a central role in histone code", which agrees with the H3K9 activity
  of human KDM4C but omits its H3K36me3 demethylase activity; no subcellular location was emitted. Non-GO
  outputs are noted, not scored.
source_documents:
- projects/PROTNLM_EVALUATION/donkey40/donkey40-predictions.tsv
- projects/PROTNLM_EVALUATION/donkey40/donkey40-evidence.tsv
- genes/EQUAS/KDM4C/KDM4C-uniprot.txt
- genes/EQUAS/KDM4C/KDM4C-goa.tsv
- genes/EQUAS/KDM4C/KDM4C-bioinformatics/human-KDM4C-Q9H3R0-uniprot.txt
- genes/EQUAS/KDM4C/KDM4C-bioinformatics/RESULTS.md
predictions:
- source_method: ProtNLM2
  source_version: UniProt API snapshot 2026-10-14
  predicted_term:
    id: GO:0140684
    label: histone H3K9me2/H3K9me3 demethylase activity
  predicted_term_type: GO_MF
  review:
    assessment: CNN
    confidence_score: 2
    summary: Human KDM4C (JMJD2C) is an experimentally characterized H3K9 tridemethylase, and JMJD2 subfamily
      members convert H3K9me3 to lower methylation states. The donkey protein is 91% identical to human KDM4C
      and conserves every 2-oxoglutarate-, Fe(II)- and Zn(2+)-binding residue of the JmjC domain, so the activity
      transfers with high confidence. The exact term is already in donkey GOA (EC 1.14.11.66 mapping), so the
      prediction is correct but not novel. Training-set membership is unknown.
    supported_by:
    - reference_id: PMID:17277772
      supporting_text: JMJD2C as the first histone tridemethylase regulating androgen receptor
    - reference_id: PMID:16603238
      supporting_text: subfamily members also functioned as trimethylation-specific demethylases
    - reference_id: file:EQUAS/KDM4C/KDM4C-bioinformatics/RESULTS.md
      supporting_text: All human 2-oxoglutarate-binding residues (Y134, N200, K208, K243), catalytic Fe-binding
        residues (H190, E192, H278) and Zn-binding residues (C236, H242, C308, C310) of the JmjC domain are
        conserved
references:
- id: PMID:17277772
  title: Cooperative demethylation by JMJD2C and LSD1 promotes androgen receptor-dependent gene expression.
  findings: []
- id: PMID:16603238
  title: Reversal of histone lysine trimethylation by the JMJD2 family of histone demethylases.
  findings: []
- id: file:EQUAS/KDM4C/KDM4C-bioinformatics/RESULTS.md
  title: 'KDM4C: donkey-human sequence comparison'
  findings: []