id: A0A9L0JGC2
gene_symbol: LNPEP
taxon:
  id: NCBITaxon:9793
  label: Equus asinus
status: COMPLETE
description: Assessment of 2 ProtNLM2 GO predictions for donkey LNPEP (A0A9L0JGC2), a type II
  membrane M1 zinc aminopeptidase that is 91% identical to human LNPEP over its full length, with
  the zinc ligands and proton acceptor conserved. Both predictions are correct but generic; the
  donkey GOA record already contains each exact term together with a more specific annotation
  (plasma membrane; metalloaminopeptidase activity), so both are scored LSP. The donkey40 snapshot
  has no non-GO ProtNLM2 output (function text or location) for this accession.
source_documents:
- projects/PROTNLM_EVALUATION/donkey40/donkey40-predictions.tsv
- genes/EQUAS/LNPEP/LNPEP-uniprot.txt
- genes/EQUAS/LNPEP/LNPEP-goa.tsv
- genes/EQUAS/LNPEP/LNPEP-bioinformatics/human_Q9UIQ6-uniprot.txt
- genes/EQUAS/LNPEP/LNPEP-bioinformatics/RESULTS.md
predictions:
- source_method: ProtNLM2
  source_version: UniProt API snapshot 2026-10-14
  predicted_term:
    id: GO:0016020
    label: membrane
  predicted_term_type: GO_CC
  review:
    assessment: LSP
    confidence_score: 2
    summary: The donkey protein has a single N-terminal transmembrane segment (110-131) and human
      LNPEP is a single-pass type II cell-membrane protein that also resides in GLUT4-containing
      intracellular vesicles. Membrane is therefore correct. Donkey GOA already contains membrane
      and the more specific plasma membrane, so the prediction is correct but less precise than
      an existing annotation.
    supported_by:
    - reference_id: file:EQUAS/LNPEP/LNPEP-bioinformatics/human_Q9UIQ6-uniprot.txt
      supporting_text: |-
        Cell membrane {ECO:0000269|PubMed:11389728};
        CC       Single-pass type II membrane protein {ECO:0000269|PubMed:11389728}.
    - reference_id: file:EQUAS/LNPEP/LNPEP-bioinformatics/RESULTS.md
      supporting_text: The donkey sequence A0A9L0JGC2 (1026 residues) aligns to human Q9UIQ6 (1025
        residues) with 91.02% identity across 1025 paired residues.
- source_method: ProtNLM2
  source_version: UniProt API snapshot 2026-10-14
  predicted_term:
    id: GO:0008237
    label: metallopeptidase activity
  predicted_term_type: GO_MF
  review:
    assessment: LSP
    confidence_score: 2
    summary: Donkey LNPEP conserves the M1 zinc ligands (H464, H468, E487) and the proton acceptor
      E465, and the human ortholog is a characterized zinc aminopeptidase that hydrolyzes oxytocin,
      vasopressin and angiotensin III. Metallopeptidase activity is therefore correct. Donkey GOA
      already contains this exact term (InterPro2GO) and its more specific descendant
      metalloaminopeptidase activity (TreeGrafter), so the prediction is less precise than an
      existing annotation.
    supported_by:
    - reference_id: file:EQUAS/LNPEP/LNPEP-bioinformatics/RESULTS.md
      supporting_text: 'ACT_SITE (Proton acceptor): human [465] E -> donkey [465] E.'
    - reference_id: file:EQUAS/LNPEP/LNPEP-bioinformatics/RESULTS.md
      supporting_text: 'BINDING (): human [468] H -> donkey [468] H.'
    - reference_id: PMID:1731608
      supporting_text: the enzyme hydrolyzed oxytocin, vasopressin, and angiotensin III
references:
- id: file:EQUAS/LNPEP/LNPEP-bioinformatics/human_Q9UIQ6-uniprot.txt
  title: UniProtKB Swiss-Prot record Q9UIQ6 (human LNPEP), fetched 2026-10-14
  findings: []
- id: file:EQUAS/LNPEP/LNPEP-bioinformatics/RESULTS.md
  title: 'LNPEP: donkey-human sequence comparison'
  findings: []
- id: PMID:1731608
  title: Identification of human placental leucine aminopeptidase as oxytocinase.
  findings: []