id: A0A9L0JBN5
gene_symbol: MITF
taxon:
  id: NCBITaxon:9793
  label: Equus asinus
status: COMPLETE
description: Assessment of the single ProtNLM2 GO prediction for donkey MITF (A0A9L0JBN5), a bHLH-Zip
  MiT/TFE transcription factor 98.4% identical to human MITF. Regulation of DNA-templated transcription
  is correct but less precise than the regulation of transcription by RNA polymerase II already in donkey
  GOA (LSP). ProtNLM2 emitted no function text or subcellular-location output for this accession.
source_documents:
- projects/PROTNLM_EVALUATION/donkey40/donkey40-predictions.tsv
- genes/EQUAS/MITF/MITF-goa.tsv
- genes/EQUAS/MITF/MITF-bioinformatics/human-O75030-uniprot.txt
- genes/EQUAS/MITF/MITF-bioinformatics/RESULTS.md
predictions:
- source_method: ProtNLM2
  source_version: UniProt API snapshot 2026-10-14
  predicted_term:
    id: GO:0006355
    label: regulation of DNA-templated transcription
  predicted_term_type: GO_BP
  review:
    assessment: LSP
    confidence_score: 2
    summary: Human MITF is a sequence-specific transcription factor that binds E-box and M-box promoter
      elements and regulates RNA polymerase II transcription of TYR and TYRP1; the donkey bHLH domain
      is 54/54 identical and the protein is 98.4% identical over paired residues. The predicted term is
      correct and is already in donkey GOA (InterPro2GO), but donkey GOA also has the more specific regulation
      of transcription by RNA polymerase II (GO:0006357, a descendant), and the gene review refines both
      rows to positive regulation of transcription by RNA polymerase II (GO:0045944) because human MITF-A
      activates the TYR and TYRP1 promoters. The prediction is therefore correct, not novel, and less precise.
    supported_by:
    - reference_id: file:EQUAS/MITF/MITF-bioinformatics/human-O75030-uniprot.txt
      supporting_text: Binds to M-boxes (5'-TCATGTG-3') and symmetrical DNA
    - reference_id: file:EQUAS/MITF/MITF-bioinformatics/RESULTS.md
      supporting_text: 'DOMAIN bHLH: human [311, 364] QL -> donkey [395, 448] QL; 54 of 54 human residues
        in this span are identical'
    - reference_id: PMID:9647758
      supporting_text: transient cotransfection assays suggested that MITF-A activated transcription of the
        tyrosinase and tyrosinase-related protein 1 genes
references:
- id: file:EQUAS/MITF/MITF-bioinformatics/human-O75030-uniprot.txt
  title: UniProt record for human MITF (O75030)
  findings: []
- id: file:EQUAS/MITF/MITF-bioinformatics/RESULTS.md
  title: 'MITF: donkey-human sequence comparison'
  findings: []
- id: PMID:9647758
  title: Identification of a novel isoform of microphthalmia-associated transcription factor that is enriched
    in retinal pigment epithelium.
  findings: []