id: A0A9L0IJ72
gene_symbol: NDEL1
taxon:
  id: NCBITaxon:9793
  label: Equus asinus
status: COMPLETE
description: Assessment of 3 ProtNLM2 GO predictions for donkey NDEL1 (A0A9L0IJ72), using the human Swiss-Prot
  record and existing human NDEL1 review, the PTHR10921 family review, and a donkey-human comparison (97.1%
  identity over the entire human sequence, with a donkey-specific C-terminal extension). Microtubule organizing
  center is correct and already in the donkey GOA record (CNN); cytoplasm is correct but less precise than
  the centrosome, spindle and axon annotations already present (LSP); cell differentiation is a generic process
  that the evidence neither supports nor refutes for NDEL1 (UNC). ProtNLM2 emitted no non-GO function-text
  or subcellular-location output for this accession in the frozen snapshot.
source_documents:
- genes/EQUAS/NDEL1/NDEL1-uniprot.txt
- genes/EQUAS/NDEL1/NDEL1-goa.tsv
- genes/human/NDEL1/NDEL1-uniprot.txt
- genes/EQUAS/NDEL1/NDEL1-bioinformatics/RESULTS.md
- projects/PROTNLM_EVALUATION/donkey40/donkey40-predictions.tsv
predictions:
- source_method: ProtNLM2
  source_version: UniProt API snapshot 2026-10-14
  predicted_term:
    id: GO:0005815
    label: microtubule organizing center
  predicted_term_type: GO_CC
  review:
    assessment: CNN
    confidence_score: 2
    summary: NDEL1 binds the interphase centrosome, the principal microtubule organizing center of animal cells,
      where it anchors microtubules. The exact term is present in the donkey GOA record (Ensembl projection
      from mouse), alongside the more specific centrosome annotation. The donkey protein is 97.1% identical
      to human NDEL1 across the full human sequence. Training-set membership is unknown.
    supported_by:
    - reference_id: file:human/NDEL1/NDEL1-uniprot.txt
      supporting_text: 'FUNCTION: Required for organization of the cellular microtubule array'
    - reference_id: file:EQUAS/NDEL1/NDEL1-bioinformatics/RESULTS.md
      supporting_text: The donkey sequence A0A9L0IJ72 (481 residues) aligns to human Q9GZM8 (345 residues)
        with 97.1% identity across 345 paired residues.
- source_method: ProtNLM2
  source_version: UniProt API snapshot 2026-10-14
  predicted_term:
    id: GO:0005737
    label: cytoplasm
  predicted_term_type: GO_CC
  review:
    assessment: LSP
    confidence_score: 2
    summary: NDEL1 is a soluble cytoplasmic and cytoskeletal protein, so cytoplasm is correct. The donkey
      GOA record already has more specific cytoplasmic locations (centrosome, microtubule organizing center,
      spindle, axon), each part of the cytoplasm, so the prediction is less precise than existing annotations.
    supported_by:
    - reference_id: file:human/NDEL1/NDEL1-uniprot.txt
      supporting_text: 'SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton,'
- source_method: ProtNLM2
  source_version: UniProt API snapshot 2026-10-14
  predicted_term:
    id: GO:0030154
    label: cell differentiation
  predicted_term_type: GO_BP
  review:
    assessment: UNC
    confidence_score: 1
    summary: NDEL1 is required for radial migration of newborn cortical neurons and, with DISC1, for regulation
      of neurite outgrowth; neither is an act of cell differentiation as such, and neither the human NDEL1
      review nor the PTHR10921 family review assigns NDEL1 a differentiation role. The term is absent from
      the donkey GOA record. Literature linking Ndel1 to neural stem cell differentiation is indirect (lentiviral
      overexpression in cultured neural stem cells, and a mitotic kinetochore role in radial glial cell-cycle
      progression that affects neurogenesis secondarily); it shows an effect on the outcome, not participation
      in differentiation. The generic prediction can be neither supported nor refuted.
    supported_by:
    - reference_id: file:human/NDEL1/NDEL1-uniprot.txt
      supporting_text: "Also required during brain\nCC       development for the migration of newly formed neurons from the\nCC       ventricular/subventricular zone toward the cortical plate."
    - reference_id: PMID:34269216
      supporting_text: "Transduction of\nneural stem cells with a lentiviral vector overexpressing Ndel1 significantly\nincreased cell proliferation and differentiation"
    - reference_id: PMID:29103808
      supporting_text: disrupting DISC1/Ndel1 complex formation prolongs mitotic length
references:
- id: file:human/NDEL1/NDEL1-uniprot.txt
  title: UniProt Swiss-Prot record for human NDEL1 (Q9GZM8)
  findings: []
- id: file:EQUAS/NDEL1/NDEL1-bioinformatics/RESULTS.md
  title: 'NDEL1: donkey-human sequence comparison'
  findings: []
- id: PMID:34269216
  title: miR-103-3p targets Ndel1 to regulate neural stem cell proliferation and differentiation.
  findings: []
- id: PMID:29103808
  title: DISC1 Regulates Neurogenesis via Modulating Kinetochore Attachment of Ndel1/Nde1 during Mitosis.
  findings: []