id: A0A9L0IQ95
gene_symbol: NUMB
taxon:
  id: NCBITaxon:9793
  label: Equus asinus
status: COMPLETE
description: Assessment of 1 ProtNLM2 GO prediction for donkey NUMB (A0A9L0IQ95), judged against the
  donkey GOA record (cytoplasm, plasma membrane, basolateral plasma membrane, cytoplasmic vesicle, positive
  regulation of neurogenesis), the human ortholog P49757 and the AAK1/endocytosis study PMID:18657069, and
  a donkey-human alignment (97% identity). The plasma membrane prediction is correct and already in donkey
  GOA (CNN). ProtNLM2 emitted no non-GO outputs (function text or subcellular location) for this accession
  in the frozen snapshot.
source_documents:
- projects/PROTNLM_EVALUATION/donkey40/donkey40-predictions.tsv
- genes/EQUAS/NUMB/NUMB-goa.tsv
- genes/EQUAS/NUMB/NUMB-bioinformatics/human-P49757-uniprot.txt
- genes/EQUAS/NUMB/NUMB-bioinformatics/RESULTS.md
predictions:
- source_method: ProtNLM2
  source_version: UniProt API snapshot 2026-10-14
  predicted_term:
    id: GO:0005886
    label: plasma membrane
  predicted_term_type: GO_CC
  review:
    assessment: CNN
    confidence_score: 2
    summary: Human NUMB localizes to the cytoplasmic face of the plasma membrane, where it acts at clathrin-coated
      pits and accumulates when AAK1 is depleted (PMID:18657069); the donkey protein is 97% identical
      to human NUMB with a conserved PTB domain. The exact term is already present in donkey GOA (UniProt
      subcellular-location mapping), and donkey GOA also carries the more specific basolateral plasma
      membrane from the PANTHER node. The prediction is therefore correct but not novel. Training-set
      membership is unknown.
    supported_by:
    - reference_id: PMID:18657069
      supporting_text: kinase depletion causes Numb to accumulate at the plasma membrane
    - reference_id: file:EQUAS/NUMB/NUMB-bioinformatics/human-P49757-uniprot.txt
      supporting_text: 'SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:18657069}'
    - reference_id: file:EQUAS/NUMB/NUMB-bioinformatics/RESULTS.md
      supporting_text: The donkey model A0A9L0IQ95 (506 aa) is 97.03% identical to human NUMB over 505
        aligned residues.
references:
- id: file:EQUAS/NUMB/NUMB-goa.tsv
  title: Donkey NUMB GOA annotations
  findings: []
- id: file:EQUAS/NUMB/NUMB-bioinformatics/human-P49757-uniprot.txt
  title: UniProt record for human NUMB (P49757)
  findings: []
- id: file:EQUAS/NUMB/NUMB-bioinformatics/RESULTS.md
  title: 'NUMB: donkey-human sequence comparison'
  findings: []
- id: PMID:18657069
  title: AAK1 regulates Numb function at an early step in clathrin-mediated endocytosis.
  findings: []