id: A0A9L0JAR4
gene_symbol: ORC5
taxon:
  id: NCBITaxon:9793
  label: Equus asinus
status: COMPLETE
description: Assessment of 2 ProtNLM2 GO predictions for donkey ORC5 product A0A9L0JAR4 (440 aa),
  one of several products of the single donkey ORC5 gene. It matches human ORC5 over residues 1-421
  (AAA+ domain with the Walker A P-loop, lid, and winged-helix with the DNA-proximal H398 hairpin) and
  ends in a 19-residue alternative C-terminal segment in place of the conserved 14-residue mammalian
  C-terminus; no known ORC5 activity or localization signal maps to those 14 residues. Nucleus is
  correct for this product and already in donkey GOA (CNN). DNA replication is correct but less
  precise than the DNA replication initiation annotation already in GOA (LSP). ProtNLM2 also emitted
  non-GO function text for this accession ("Component of the origin recognition complex (ORC) that
  binds origins of replication. DNA-binding is ATP-dependent ... ORC is required to assemble the
  pre-replication complex necessary to initiate DNA replication"), which closely mirrors the existing
  ARBA function text of the donkey record and is not scored here.
source_documents:
- projects/PROTNLM_EVALUATION/donkey40/donkey40-predictions.tsv
- genes/EQUAS/ORC5/ORC5-uniprot.txt
- genes/EQUAS/ORC5/ORC5-goa.tsv
- genes/EQUAS/ORC5/ORC5-bioinformatics/human_O43913-uniprot.txt
- genes/EQUAS/ORC5/ORC5-bioinformatics/RESULTS.md
- genes/EQUAS/ORC5/ORC5-bioinformatics/RESULTS-rereview.md
predictions:
- source_method: ProtNLM2
  source_version: UniProt API snapshot 2026-10-14
  predicted_term:
    id: GO:0006260
    label: DNA replication
  predicted_term_type: GO_BP
  review:
    assessment: LSP
    confidence_score: 2
    summary: Human ORC5 is a subunit of ORC, which binds origins and is required to assemble the
      pre-replication complex that initiates DNA replication. This product, A0A9L0JAR4, keeps the
      AAA+ domain with the Walker A P-loop (GHTASGKT), the lid and the winged-helix with the
      DNA-proximal H398, matching human ORC5 at 402 of residues 1-420; it lacks only the last 14
      residues of the canonical C-terminus, which are replaced by an alternative segment and have no
      known role in ORC assembly or replication. DNA replication is therefore correct for this
      product. Donkey GOA contains this exact term (combined IEA) and the more specific descendant
      DNA replication initiation (TreeGrafter), so the prediction is less precise than an existing
      annotation.
    supported_by:
    - reference_id: file:EQUAS/ORC5/ORC5-bioinformatics/human_O43913-uniprot.txt
      supporting_text: |-
        ORC is required to assemble the pre-replication complex necessary
        CC       to initiate DNA replication.
    - reference_id: file:EQUAS/ORC5/ORC5-bioinformatics/RESULTS-rereview.md
      supporting_text: 'Human ORC5 Walker A (residues 37-44) GHTASGKT vs A0A9L0JAR4 GHTASGKT; human DNA-proximal
        beta-hairpin H398 vs A0A9L0JAR4 residue 398 H.'
    - reference_id: file:EQUAS/ORC5/ORC5-bioinformatics/RESULTS-rereview.md
      supporting_text: 'Residues 1-420: 402/420 identical to human; human 421-435 RTVNFDIIKYLYDFL vs A0A9L0JAR4
        421-440 RGPPKTCLQTHKNRLTMSSH.'
- source_method: ProtNLM2
  source_version: UniProt API snapshot 2026-10-14
  predicted_term:
    id: GO:0005634
    label: nucleus
  predicted_term_type: GO_CC
  review:
    assessment: CNN
    confidence_score: 2
    summary: Human ORC5 is experimentally localized to the nucleus and chromatin (PMID:9765232;
      PMID:31160578 via UniProt), and ORC acts on nuclear replication origins. Human ORC5 has no
      annotated nuclear localization signal in its C-terminal 14 residues, the only canonical region
      this product A0A9L0JAR4 lacks; it retains the AAA+, lid and winged-helix regions that build the
      ORC2-5 core, so nuclear localization is expected for this product. The exact term is already in
      donkey GOA (combined IEA), so the prediction is correct but not novel.
    supported_by:
    - reference_id: file:EQUAS/ORC5/ORC5-bioinformatics/human_O43913-uniprot.txt
      supporting_text: 'SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:31160578}.'
    - reference_id: PMID:9765232
      supporting_text: The bulk of HsORC5p is in an insoluble nuclear fraction
    - reference_id: PMID:32808929
      supporting_text: The Origin Recognition Complex (ORC) is necessary for orchestrating the initiation
        process by binding to origin DNA, recruiting CDC6, and assembling the MCM replicative helicase
        on DNA.
references:
- id: file:EQUAS/ORC5/ORC5-bioinformatics/human_O43913-uniprot.txt
  title: UniProtKB Swiss-Prot record O43913 (human ORC5), fetched 2026-10-14
  findings: []
- id: file:EQUAS/ORC5/ORC5-bioinformatics/RESULTS.md
  title: 'ORC5: donkey-human sequence comparison'
  findings: []
- id: file:EQUAS/ORC5/ORC5-bioinformatics/RESULTS-rereview.md
  title: ORC5 donkey gene models vs human/mouse/horse ORC5 (re-review)
  findings: []
- id: PMID:32808929
  title: The dynamic nature of the human origin recognition complex revealed through five cryoEM
    structures.
  findings: []
- id: PMID:9765232
  title: ORC5L, a new member of the human origin recognition complex, is deleted in uterine leiomyomas
    and malignant myeloid diseases.
  findings: []