id: A0A9L0IQB9
gene_symbol: SEC23A
taxon:
  id: NCBITaxon:9793
  label: Equus asinus
status: COMPLETE
description: 'Assessment of 1 ProtNLM2 GO prediction for donkey SEC23A (A0A9L0IQB9), judged against the
  donkey GOA record (GTPase activator activity, COPII vesicle coat, ER to Golgi vesicle-mediated transport,
  COPII-coated vesicle budding and cargo loading, ER membrane, ER exit site, cytosol), the re-reviewed
  donkey gene review, the human ortholog Q15436, budding assays on human SEC23A (PMID:17981132) and yeast
  Sec23p (PMID:8451644), a donkey-human alignment (99% identity) and a comparison with the canonical
  donkey product A0A9L0JMP6. This accession is an exon-skipping transcript product whose 26-residue
  deletion falls inside the Sec23/Sec24 helical domain, so COPII-coated vesicle budding, a correct and
  already-annotated process for the gene, is uncertain for this product (UNC). Non-GO ProtNLM2 output, not scored: subcellular
  location ''Membrane'', which is true but much less informative than the curated peripheral ER-membrane
  and COPII-vesicle-membrane locations. No function text was emitted for this accession.'
source_documents:
- projects/PROTNLM_EVALUATION/donkey40/donkey40-predictions.tsv
- genes/EQUAS/SEC23A/SEC23A-goa.tsv
- genes/EQUAS/SEC23A/SEC23A-bioinformatics/human-Q15436-uniprot.txt
- genes/EQUAS/SEC23A/SEC23A-bioinformatics/RESULTS.md
predictions:
- source_method: ProtNLM2
  source_version: UniProt API snapshot 2026-10-14
  predicted_term:
    id: GO:0090114
    label: COPII-coated vesicle budding
  predicted_term_type: GO_BP
  review:
    assessment: UNC
    confidence_score: 1
    summary: COPII-coated vesicle budding is a core process of the SEC23A gene, whose canonical product
      is an inner-coat COPII subunit and the SAR1 GTPase-activating protein; Sec23p-Sar1p interaction is
      required for budding in yeast, and a disease variant of human SEC23A that poorly recruits Sec13-Sec31
      inhibits vesicle formation in vitro. The term is already in donkey GOA for this gene (InterPro2GO
      mapping of the Sec23 C-terminal domain), so it is not novel. The scored sequence, A0A9L0IQB9, is an
      in-frame exon-skipping transcript product that lacks the 26 residues equivalent to human 554-579.
      It keeps the zinc finger and its four Cys ligands, the trunk domain with F382, the beta-sandwich
      domain and the gelsolin-like PPP-motif-binding residues, but the deletion falls inside the
      Sec23/Sec24 helical domain. That domain spans residues 518-616 in the canonical donkey product
      A0A9L0JMP6, while its Pfam match on A0A9L0IQB9 covers only 553-590. Whether a Sec23 with a
      truncated helical domain still folds, binds SEC24 and SEC31 and supports vesicle budding has not
      been tested, and there is no expression evidence for this transcript. Because the required
      feature, an intact Sec23 fold, cannot be confirmed for this product, the prediction is
      uncertain at product level.
    supported_by:
    - reference_id: file:EQUAS/SEC23A/SEC23A-bioinformatics/human-Q15436-uniprot.txt
      supporting_text: deformation of the endoplasmic reticulum membrane into vesicles
    - reference_id: PMID:17981132
      supporting_text: In vitro assays reveal that the mutant form of SEC23A poorly recruits the Sec13-Sec31
        complex, inhibiting vesicle formation.
    - reference_id: PMID:8451644
      supporting_text: an interaction between Sar1p and the Sec23p subunit of an oligomeric protein is also
        required for vesicle budding
    - reference_id: file:EQUAS/SEC23A/SEC23A-bioinformatics/RESULTS-rereview.md
      supporting_text: lacks the 26-residue segment equivalent to human 554-579
    - reference_id: file:EQUAS/SEC23A__A0A9L0JMP6/SEC23A__A0A9L0JMP6-uniprot.txt
      supporting_text: DOMAIN          518..616
    - reference_id: file:EQUAS/SEC23A/SEC23A-uniprot.txt
      supporting_text: DOMAIN          553..590
references:
- id: file:EQUAS/SEC23A/SEC23A-goa.tsv
  title: Donkey SEC23A GOA annotations
  findings: []
- id: file:EQUAS/SEC23A/SEC23A-bioinformatics/human-Q15436-uniprot.txt
  title: UniProt record for human SEC23A (Q15436)
  findings: []
- id: file:EQUAS/SEC23A/SEC23A-bioinformatics/RESULTS.md
  title: 'SEC23A: donkey-human sequence comparison'
  findings: []
- id: file:EQUAS/SEC23A/SEC23A-bioinformatics/RESULTS-rereview.md
  title: 'SEC23A (donkey): gene-model comparison for the 2026-10-21 re-review'
  findings: []
- id: file:EQUAS/SEC23A/SEC23A-uniprot.txt
  title: UniProt record for donkey SEC23A transcript product A0A9L0IQB9
  findings: []
- id: file:EQUAS/SEC23A__A0A9L0JMP6/SEC23A__A0A9L0JMP6-uniprot.txt
  title: UniProt record for donkey SEC23A canonical product A0A9L0JMP6
  findings: []
- id: PMID:21235735
  title: Molecular analysis and intestinal expression of SAR1 genes and proteins in Anderson's disease
    (Chylomicron retention disease).
  findings: []
- id: PMID:17981132
  title: The genetic basis of a craniofacial disease provides insight into COPII coat assembly.
  findings: []
- id: PMID:8451644
  title: Requirement for a GTPase-activating protein in vesicle budding from the endoplasmic reticulum.
  findings: []