id: A0A9L0JNL2
gene_symbol: SLC27A5
taxon:
  id: NCBITaxon:9793
  label: Equus asinus
status: COMPLETE
description: 'Assessment of 1 ProtNLM2 GO prediction for donkey SLC27A5 (A0A9L0JNL2), judged against the
  donkey GOA record (long-chain, very-long-chain and cholate CoA-ligase activities; long-chain fatty acid,
  very long-chain fatty acid and bile acid metabolic processes; ER membrane and others), the human ortholog
  Q9Y2P5 with its enzyme characterization (PMID:10749848, PMID:10479480), and a donkey-human alignment (81%
  identity over the full human length). Lipid metabolic process is correct but much less specific than
  the curated process annotations (LSP). Non-GO ProtNLM2 outputs, not scored: subcellular locations ''Endoplasmic
  reticulum membrane'' (agrees with the human experimental localization) and ''Membrane'' (true but uninformative).
  No function text was emitted for this accession.'
source_documents:
- projects/PROTNLM_EVALUATION/donkey40/donkey40-predictions.tsv
- genes/EQUAS/SLC27A5/SLC27A5-goa.tsv
- genes/human/SLC27A5/SLC27A5-uniprot.txt
- genes/EQUAS/SLC27A5/SLC27A5-bioinformatics/RESULTS.md
predictions:
- source_method: ProtNLM2
  source_version: UniProt API snapshot 2026-10-14
  predicted_term:
    id: GO:0006629
    label: lipid metabolic process
  predicted_term_type: GO_BP
  review:
    assessment: LSP
    confidence_score: 2
    summary: SLC27A5 activates bile acids and long- and very-long-chain fatty acids to their CoA thioesters
      (human data, PMID:10749848 and PMID:10479480), and the donkey protein retains the AMP-binding catalytic
      domain at 81% identity, so participation in lipid metabolism is correct. Donkey GOA already carries
      the more specific descendants long-chain fatty acid metabolic process, very long-chain fatty acid
      metabolic process and bile acid metabolic process, so the prediction is correct but less precise
      than existing annotations. Training-set membership is unknown.
    supported_by:
    - reference_id: PMID:10749848
      supporting_text: exhibited cholate:CoA ligase (choloyl-CoA synthetase) activity with both
    - reference_id: PMID:10479480
      supporting_text: For all substrates tested (C18:0, C20:0, C24:0, C26:0)
    - reference_id: file:EQUAS/SLC27A5/SLC27A5-bioinformatics/RESULTS.md
      supporting_text: The donkey model A0A9L0JNL2 (700 aa) is 81.30% identical to human SLC27A5 over 690
        aligned residues, covering the full human sequence
references:
- id: file:EQUAS/SLC27A5/SLC27A5-goa.tsv
  title: Donkey SLC27A5 GOA annotations
  findings: []
- id: file:human/SLC27A5/SLC27A5-uniprot.txt
  title: UniProt record for human SLC27A5 (Q9Y2P5)
  findings: []
- id: file:EQUAS/SLC27A5/SLC27A5-bioinformatics/RESULTS.md
  title: 'SLC27A5: donkey-human sequence comparison'
  findings: []
- id: PMID:10749848
  title: The human liver-specific homolog of very long-chain acyl-CoA synthetase is cholate:CoA ligase.
  findings: []
- id: PMID:10479480
  title: 'Human liver-specific very-long-chain acyl-coenzyme A synthetase: cDNA cloning and characterization
    of a second enzymatically active protein.'
  findings: []