| Category | TBC1D14 feature | Specific details | Functional implication | Key evidence/citations |
|---|---|---|---|---|
| Gene/protein identity | Atlantic cod target from UniProt | UniProt A0A8C5FPT8 is annotated as **Rab-GAP TBC domain-containing protein**, gene **tbc1d14**, from **Gadus morhua**; direct species-specific literature was not found in the retrieved corpus, so functional interpretation relies on conserved TBC1D14 studies from other vertebrates plus the conserved Rab-GAP TBC domain annotation | Supports cautious homology-based functional inference rather than direct cod-specific experimental assignment | (pqac-00000002, pqac-00000012) |
| Domain organization | C-terminal TBC domain | TBC1D14 has a **typical TBC-domain protein architecture** with the **TBC domain at the C-terminus**; one construct spanning **aa 224–669** includes the C-terminal region and the **putative TBC domain from aa 411–611** | Establishes membership in the TBC Rab-regulator family and suggests potential Rab-related regulatory capacity | (pqac-00000001) |
| Domain organization | TRAPP-binding region (TBR) | A **103 aa N-terminal region, aa 120–223**, is **sufficient for TRAPP interaction** and is termed the **TRAPP-binding region (TBR)** | Provides the physical basis for recruitment of TRAPP/TRAPPIII machinery by TBC1D14 | (pqac-00000001, pqac-00000007) |
| Domain organization | ULK1-binding region | The **ULK1-interacting region** is distinct from the TBR and lies within **aa 224–330** | Indicates modular organization: separate surfaces for autophagy kinase interaction and TRAPP binding | (pqac-00000001) |
| Catalytic/mechanistic context | TBC family catalytic mechanism | TBC Rab-GAP proteins generally regulate Rab GTPases via a **dual-finger mechanism** using conserved catalytic residues in the TBC domain; structurally related TBC proteins require catalytic **arginine/Q-finger glutamine** residues for GAP activity | Provides the mechanistic baseline for interpreting the conserved cod TBC domain, while not proving TBC1D14 itself is an active Rab GAP toward Rab11 | (pqac-00000011, pqac-00000012) |
| Primary molecular function | RAB11 binding rather than Rab11 GAP activity | TBC1D14 **binds RAB11** and behaves as a **RAB11 effector**; the literature summarized in the retrieved texts states that TBC1D14 **does not appear to act as a GAP for RAB11** | Suggests its primary role is scaffolding/coordination of membrane traffic rather than direct inactivation of Rab11 | (pqac-00000003, pqac-00000009) |
| Protein interactors | TRAPP core and associated subunits | TBC1D14 co-immunoprecipitates with **TRAPPC4**, **TRAPPC12**, and via TRAPPC3 pulldown is associated with endogenous TBC1D14; BioID and knockdown data indicate **TRAPPC8** is the most proximal subunit mediating the interaction | Defines TBC1D14 as a recruiter/partner of a mammalian **TRAPPIII-like complex** | (pqac-00000003, pqac-00000007) |
| Protein interactors | TRAPPC8 | **TRAPPC8** is required for TBC1D14/TBR to bind the core TRAPP complex and is identified as the likely mammalian ortholog of yeast **Trs85**, the autophagy-specific TRAPPIII subunit | Places TBC1D14 in a Rab1-regulatory, autophagy-relevant tethering pathway | (pqac-00000007, pqac-00000010) |
| Protein interactors | RAB11A/B | TBC1D14-induced tubules are **RAB11-positive**; prior work cited in the paper established that endogenous RAB11 is required for tubule formation, and RAB11 recruits TBC1D14 to recycling endosome membranes | Supports a model in which TBC1D14 is a RAB11-dependent membrane trafficking factor at recycling endosomes | (pqac-00000001, pqac-00000003) |
| Protein interactors | RAB1B | Endogenous **RAB1B** partially colocalizes with TBC1D14 at the Golgi; a subset of TBC1D14-induced tubules simultaneously harbors **RAB11A and RAB1B** | Supports a Rab conversion/hand-off model linking RAB11-positive recycling endosomes to RAB1-positive early Golgi/ERGIC traffic | (pqac-00000001, pqac-00000003, pqac-00000009) |
| Protein interactors | ULK1 | TBC1D14 overexpression was previously linked to a **RAB11- and ULK1-positive recycling endosome** compartment; ULK1-binding region is distinct from the TRAPP-binding region | Connects TBC1D14 to early autophagy signaling and membrane supply pathways | (pqac-00000000, pqac-00000003) |
| Protein interactors | ATG9 / ATG9-positive membranes | ATG9-positive membranes co-isolate with **TRAPPC8** and **TRAPPC4**; TBC1D14/TRAPP activity is required for proper **ATG9** localization and cycling | Links TBC1D14 indirectly and functionally to the ATG9 membrane cycle essential for autophagosome biogenesis | (pqac-00000010, pqac-00000009) |
| Subcellular localization | Recycling endosomes | TBC1D14 overexpression generates a **tubulated transferrin-positive recycling endosome** compartment; endogenous TBC1D14 also localizes to peripheral recycling endosome structures | Indicates action on endocytic recycling membranes, particularly **RAB11-positive** compartments | (pqac-00000003, pqac-00000010) |
| Subcellular localization | Golgi / early Golgi | Endogenous TBC1D14 localizes to the **Golgi stack** and partially colocalizes with **TRAPPC4** and **RAB1B**; overexpression fragments the **GM130-positive cis-Golgi** | Positions TBC1D14 at the interface of autophagy-related trafficking and Golgi organization/secretory traffic | (pqac-00000001, pqac-00000000, pqac-00000010) |
| Subcellular localization | Transport intermediate between RE and RAB1 compartments | TBC1D14-positive tubules can harbor **RAB11A, RAB1B, and TRAPPC4** simultaneously, consistent with a **tubulo-vesicular intermediate** between recycling endosomes and early Golgi/ERGIC membranes | Suggests TBC1D14 coordinates membrane exchange between endocytic recycling and early secretory systems | (pqac-00000003, pqac-00000009) |
| Primary molecular function | Recruitment of TRAPP/TRAPPIII | TBC1D14 binds TRAPP via its **aa 120–223 TBR**, and **TRAPPC8** mediates this interaction; TBC1D14 overexpression mislocalizes TRAPP to tubulated recycling endosomes | Core scaffolding/adaptor role: recruits tethering/GEF machinery to specific membranes | (pqac-00000001, pqac-00000007) |
| Primary molecular function | Promotion of RAB1 activation indirectly through TRAPP | TRAPP is the **GEF for RAB1**, and TBC1D14 is **required but not sufficient** for normal RAB1 activation; depletion of TBC1D14 reduces RAB1B binding to the effector golgin-84 | TBC1D14 helps position/enable TRAPPIII-mediated **RAB1 activation** rather than directly catalyzing RAB1 nucleotide exchange itself | (pqac-00000006, pqac-00000007) |
| Primary molecular function | Coordination of membrane traffic for autophagosome formation | Overexpression of TBC1D14 or its TBR inhibits **LC3 lipidation**, reduces **LC3 puncta**, and also reduces early autophagy markers **WIPI2** and **DFCP1** | Establishes TBC1D14 as a regulator of an **early step** in autophagosome biogenesis | (pqac-00000000, pqac-00000007) |
| Biological process | Autophagy regulation | TBC1D14 is described as a **negative regulator of autophagy** that controls membrane delivery from **RAB11-positive recycling endosomes** to forming autophagosomes | Principal characterized biological role in the literature | (pqac-00000002, pqac-00000003) |
| Biological process | ATG9 trafficking | TBC1D14 and TRAPPIII regulate **ATG9 trafficking independently of ULK1**; disruption of TBC1D14/TRAPP/RAB1 function disperses juxtanuclear ATG9 and alters the cycling ATG9 pool | Explains how TBC1D14 influences autophagy mechanistically: by maintaining the membrane itinerary of the only transmembrane core ATG protein | (pqac-00000002, pqac-00000010) |
| Biological process | Secretory pathway and Golgi integrity | TBR overexpression impairs **constitutive secretion** and fragments the Golgi; **TRAPPC8 depletion** phenocopies Golgi and secretory defects | Indicates TBC1D14 also regulates **ER-Golgi/early secretory traffic** beyond autophagy | (pqac-00000000, pqac-00000007) |
| Biological process | RE-to-Golgi membrane exchange | The authors propose a constitutive trafficking step from **peripheral recycling endosomes to the early Golgi** that maintains an ATG9 cycling pool needed for autophagy initiation | Integrates endocytic recycling and early secretory trafficking into a unified TBC1D14 function | (pqac-00000002, pqac-00000009) |
| Cod-specific inference | Most likely conserved functional interpretation for Gadus morhua tbc1d14 | Because cod-specific experiments were not retrieved, the safest annotation is that Atlantic cod Tbc1d14 is likely an **intracellular membrane-trafficking regulator** associated with the **Rab-GAP TBC domain family**, probably functioning at **recycling endosome/Golgi interfaces** and in **autophagy-related ATG9 trafficking**, but direct substrate specificity and cod-specific localization remain unvalidated | Provides a bounded, evidence-based annotation for A0A8C5FPT8 without overclaiming species-specific function | (pqac-00000001, pqac-00000002, pqac-00000009) |


*Table: This table summarizes the best-supported characteristics of TBC1D14 relevant to annotating Atlantic cod tbc1d14, including domains, interactors, localization, functions, and pathways. It is especially useful because direct Gadus morhua literature was not retrieved, so annotation depends on careful transfer from conserved vertebrate evidence.*