# AFAP1L2 re-review: horse gene models vs human Q8N4X5

Generated by `compare_sequences_rereview.py` (Biopython global alignment, BLOSUM62, gap open -10, extend -0.5, free end gaps). Inputs: horse A0A9L0RQI4 UniProt text, human Q8N4X5 UniProt text, mouse Q5DTU0 and ten horse AFAP1L2 TrEMBL FASTA records downloaded from UniProt REST.

## Summary per sequence

| Sequence | Length | Paired with human | Identity | Human residues missing | Insertions >=3 aa (after human pos:len) | First 20 aa |
|---|---|---|---|---|---|---|
| A0A9L0RQI4 (benchmark) | 850 | 818 | 0.885 | 0 | 0:11, 652:20 | MRSTMWVLLANTATSNALEQ |
| mouse Q5DTU0 | 825 | 818 | 0.835 | 0 | 597:5 | MERYKALEQLLTELDDFLKV |
| horse A0A3Q2GYR5 | 816 | 815 | 0.887 | 3 | none | MTPLEQLLTELDDFLKILDQ |
| horse A0A3Q2GZU2 | 936 | 802 | 0.860 | 16 | 0:11, 818:122 | MRSTMWVLLANTATSNALEQ |
| horse A0A3Q2HBW2 | 943 | 802 | 0.867 | 16 | 6:18, 818:122 | MERYKAQGCCCLVVQRRILQ |
| horse A0A3Q2I027 | 792 | 762 | 0.887 | 56 | 135:28 | MNKVTVHKQQNAESQDKVPE |
| horse A0A3Q2I7D4 | 856 | 818 | 0.886 | 0 | 0:36 | MRFATPSCPPTRQAPDAPER |
| horse A0A9L0RB13 | 748 | 719 | 0.867 | 99 | 82:28 | MLPHLGLSLYLCTCICPSLH |
| horse A0A9L0RVT8 | 898 | 818 | 0.884 | 0 | 0:79 | MEKAVAWEPGDPRVLFPGTG |
| horse A0A9L0T7E5 | 762 | 760 | 0.889 | 58 | none | MERYKALEQLLTELDDFLKI |
| horse A0A9L0TGF9 | 884 | 818 | 0.884 | 0 | 0:65 | MERYKGSLSQPLMHCQALTI |

## Human feature conservation in benchmark A0A9L0RQI4

| Human feature | Horse positions | Paired / length | Identical |
|---|---|---|---|
| N-terminal SH2/SH3-binding region (1-167) | 12-178 | 167/167 | 145/167 |
| Tyr54 (p85 SH2-binding phosphosite) | 65-65 | 1/1 | 1/1 |
| PH 1 (175-271) | 186-282 | 97/97 | 95/97 |
| PH 2 (353-447) | 364-458 | 95/95 | 93/95 |
| Coiled coil (652-749) | 664-781 | 98/98 | 90/98 |
| C-terminal 63 aa (756-818) | 788-850 | 63/63 | 57/63 |