id: A0A9L0RQI4 gene_symbol: AFAP1L2 taxon: id: NCBITaxon:9796 label: Equus caballus status: COMPLETE description: The nine predictions are supported as conserved adaptor-mediated signaling functions or downstream processes, with cytokine and proliferation effects interpreted in their experimental cellular contexts. source_documents: - projects/PROTNLM_EVALUATION/mammal-benchmark/horse40-predictions.csv - projects/PROTNLM_EVALUATION/mammal-benchmark/predictions.jsonl.gz - projects/PROTNLM_EVALUATION/mammal-benchmark/paired-sequences/AFAP1L2.json references: - id: file:human/AFAP1L2/AFAP1L2-Xu2007-excerpt.md title: human/AFAP1L2/AFAP1L2-Xu2007-excerpt.md findings: [] - id: file:HORSE/AFAP1L2/AFAP1L2-uniprot.txt title: HORSE/AFAP1L2/AFAP1L2-uniprot.txt findings: [] - id: file:HORSE/AFAP1L2/AFAP1L2-bioinformatics/RESULTS.md title: HORSE/AFAP1L2/AFAP1L2-bioinformatics/RESULTS.md findings: [] - id: PMID:17412687 title: XB130, a novel adaptor protein for signal transduction. full_text_unavailable: true findings: [] predictions: - source_method: ProtNLM2 source_version: UniProt API snapshot 2026-09-08 predicted_term: id: GO:0032675 label: regulation of interleukin-6 production predicted_term_type: GO_BP review: assessment: COR confidence_score: 2 summary: The selected horse AFAP1L2 aligns across all 818 human residues with 88.6% identity among paired positions and retains its AFAP-family architecture. The primary human airway-cell experiment reports reduced IL-6 after XB130 siRNA. Conservation of the signaling adaptor supports this general regulatory participation; it does not establish identical effect size or expression across horse tissues. The claim is new relative to the frozen horse GOA; the model training membership is unknown. supported_by: - &id005 reference_id: file:human/AFAP1L2/AFAP1L2-Xu2007-excerpt.md supporting_text: In addition to IL-8, the protein level of IL-6 was also reduced by siRNA treatment. - &id001 reference_id: file:HORSE/AFAP1L2/AFAP1L2-uniprot.txt supporting_text: DR InterPro; IPR030113; AFAP. - &id002 reference_id: file:HORSE/AFAP1L2/AFAP1L2-bioinformatics/RESULTS.md supporting_text: share 88.6% identity among 818 paired residues. - source_method: ProtNLM2 source_version: UniProt API snapshot 2026-09-08 predicted_term: id: GO:0045893 label: positive regulation of DNA-templated transcription predicted_term_type: GO_BP review: assessment: COR confidence_score: 2 summary: The selected horse AFAP1L2 aligns across all 818 human residues with 88.6% identity among paired positions and retains its AFAP-family architecture. Human XB130 enhances Src-dependent SRE/AP-1 transcription. This supports conserved upstream positive regulation, without implying that AFAP1L2 is a DNA-binding transcription factor. The claim is new relative to the frozen horse GOA; the model training membership is unknown. supported_by: - reference_id: PMID:17412687 supporting_text: 'XB130 expression in HEK293 cells enhanced serum response element- and AP-1-dependent transcriptional activation mediated by c-Src.' - *id001 - *id002 - source_method: ProtNLM2 source_version: UniProt API snapshot 2026-09-08 predicted_term: id: GO:0007346 label: regulation of mitotic cell cycle predicted_term_type: GO_BP review: assessment: COR confidence_score: 2 summary: The selected horse AFAP1L2 aligns across all 818 human residues with 88.6% identity among paired positions and retains its AFAP-family architecture. XB130 knockdown alters human epithelial cell-cycle progression downstream of growth-factor signaling. This supports a conserved regulatory contribution, not intrinsic cell-cycle catalysis. The claim is new relative to the frozen horse GOA; the model training membership is unknown. supported_by: - &id003 reference_id: PMID:17412687 supporting_text: 'Down-regulation of endogenous XB130 with siRNA reduced c-Src activity, IL-8 production, EGF-induced phosphorylation of Akt and GSK3beta, and altered cell cycles in human lung epithelial cells.' - *id001 - *id002 - source_method: ProtNLM2 source_version: UniProt API snapshot 2026-09-08 predicted_term: id: GO:0030296 label: protein tyrosine kinase activator activity predicted_term_type: GO_MF review: assessment: COR confidence_score: 2 summary: The selected horse AFAP1L2 aligns across all 818 human residues with 88.6% identity among paired positions and retains its AFAP-family architecture. The human protein activates Src and depletion reduces endogenous Src activity. Conserved adaptor architecture supports kinase-activator activity rather than kinase activity. The claim is new relative to the frozen horse GOA; the model training membership is unknown. supported_by: - reference_id: PMID:17412687 supporting_text: 'Their co-expression in COS-7 cells resulted in activation of c-Src and elevated tyrosine phosphorylation of multiple proteins, including XB130 itself.' - *id003 - *id001 - *id002 - source_method: ProtNLM2 source_version: UniProt API snapshot 2026-09-08 predicted_term: id: GO:0032757 label: positive regulation of interleukin-8 production predicted_term_type: GO_BP review: assessment: COR confidence_score: 2 summary: The selected horse AFAP1L2 aligns across all 818 human residues with 88.6% identity among paired positions and retains its AFAP-family architecture. Human airway-cell knockdown lowers IL-8 production. The horse inference concerns a conserved signaling contribution to cytokine output and remains tissue/context dependent. The claim is new relative to the frozen horse GOA; the model training membership is unknown. supported_by: - *id003 - *id001 - *id002 - source_method: ProtNLM2 source_version: UniProt API snapshot 2026-09-08 predicted_term: id: GO:0042169 label: SH2 domain binding predicted_term_type: GO_MF review: assessment: COR confidence_score: 2 summary: The selected horse AFAP1L2 aligns across all 818 human residues with 88.6% identity among paired positions and retains its AFAP-family architecture. The human N-terminal motif-rich region supports SH2-mediated interaction and Src signaling. Retention of the human-length sequence and adaptor architecture supports transfer, rather than assigning an SH2 domain to AFAP1L2. The claim is new relative to the frozen horse GOA; the model training membership is unknown. supported_by: - &id004 reference_id: PMID:17412687 supporting_text: 'XB130DeltaN, an N-terminal deletion mutant lacking a putative SH3-binding motif and several putative SH2-binding sites, reduced its ability to mediate Src signal transduction.' - *id001 - *id002 - source_method: ProtNLM2 source_version: UniProt API snapshot 2026-09-08 predicted_term: id: GO:0017124 label: SH3 domain binding predicted_term_type: GO_MF review: assessment: COR confidence_score: 2 summary: The selected horse AFAP1L2 aligns across all 818 human residues with 88.6% identity among paired positions and retains its AFAP-family architecture. The human N-terminal SH3-binding region mediates signaling interactions. This molecular binding role is consistent with the conserved horse adaptor. The claim is new relative to the frozen horse GOA; the model training membership is unknown. supported_by: - *id004 - *id001 - *id002 - source_method: ProtNLM2 source_version: UniProt API snapshot 2026-09-08 predicted_term: id: GO:0006954 label: inflammatory response predicted_term_type: GO_BP review: assessment: COR confidence_score: 2 summary: The selected horse AFAP1L2 aligns across all 818 human residues with 88.6% identity among paired positions and retains its AFAP-family architecture. Primary human cytokine-response experiments establish a contribution to inflammatory signaling. This is a broad, context-dependent biological process, not a claim of universal proinflammatory action. The claim is new relative to the frozen horse GOA; the model training membership is unknown. supported_by: - *id003 - *id005 - *id001 - *id002 - source_method: ProtNLM2 source_version: UniProt API snapshot 2026-09-08 predicted_term: id: GO:0045742 label: positive regulation of epidermal growth factor receptor signaling pathway predicted_term_type: GO_BP review: assessment: COR confidence_score: 2 summary: The selected horse AFAP1L2 aligns across all 818 human residues with 88.6% identity among paired positions and retains its AFAP-family architecture. Human depletion reduces EGF-induced Akt/GSK3beta phosphorylation, supporting a conserved positive contribution to EGFR downstream signaling through the adaptor. The claim is new relative to the frozen horse GOA; the model training membership is unknown. supported_by: - *id003 - *id001 - *id002