ID   A0A9L0RQI4_HORSE        Unreviewed;       850 AA.
AC   A0A9L0RQI4;
DT   13-SEP-2023, integrated into UniProtKB/TrEMBL.
DT   13-SEP-2023, sequence version 1.
DT   02-SEP-2026, entry version 14.
DE   RecName: Full=Actin filament-associated protein 1-like 2 {ECO:0000256|ARBA:ARBA00072612};
GN   Name=AFAP1L2 {ECO:0000313|Ensembl:ENSECAP00000066139.1};
OS   Equus caballus (Horse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Perissodactyla; Equidae; Equus.
OX   NCBI_TaxID=9796 {ECO:0000313|Ensembl:ENSECAP00000066139.1, ECO:0000313|Proteomes:UP000002281};
RN   [1] {ECO:0000313|Ensembl:ENSECAP00000066139.1, ECO:0000313|Proteomes:UP000002281}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Thoroughbred {ECO:0000313|Ensembl:ENSECAP00000066139.1,
RC   ECO:0000313|Proteomes:UP000002281};
RX   PubMed=19892987; DOI=10.1126/science.1178158;
RG   Broad Institute Genome Sequencing Platform;
RG   Broad Institute Whole Genome Assembly Team;
RA   Wade C.M., Giulotto E., Sigurdsson S., Zoli M., Gnerre S., Imsland F.,
RA   Lear T.L., Adelson D.L., Bailey E., Bellone R.R., Bloecker H., Distl O.,
RA   Edgar R.C., Garber M., Leeb T., Mauceli E., MacLeod J.N., Penedo M.C.T.,
RA   Raison J.M., Sharpe T., Vogel J., Andersson L., Antczak D.F., Biagi T.,
RA   Binns M.M., Chowdhary B.P., Coleman S.J., Della Valle G., Fryc S.,
RA   Guerin G., Hasegawa T., Hill E.W., Jurka J., Kiialainen A., Lindgren G.,
RA   Liu J., Magnani E., Mickelson J.R., Murray J., Nergadze S.G., Onofrio R.,
RA   Pedroni S., Piras M.F., Raudsepp T., Rocchi M., Roeed K.H., Ryder O.A.,
RA   Searle S., Skow L., Swinburne J.E., Syvaenen A.C., Tozaki T., Valberg S.J.,
RA   Vaudin M., White J.R., Zody M.C., Lander E.S., Lindblad-Toh K.;
RT   "Genome sequence, comparative analysis, and population genetics of the
RT   domestic horse.";
RL   Science 326:865-867(2009).
RN   [2] {ECO:0000313|Ensembl:ENSECAP00000066139.1}
RP   IDENTIFICATION.
RC   STRAIN=Thoroughbred {ECO:0000313|Ensembl:ENSECAP00000066139.1};
RG   Ensembl;
RL   Submitted (JAN-2026) to UniProtKB.
CC   -!- FUNCTION: May play a role in a signaling cascade by enhancing the
CC       kinase activity of SRC. Contributes to SRC-regulated transcription
CC       activation. {ECO:0000256|ARBA:ARBA00059761}.
CC   -!- SUBUNIT: Interacts with SRC. Interacts with LCK when tyrosine
CC       phosphorylated. {ECO:0000256|ARBA:ARBA00061961}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
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DR   AlphaFoldDB; A0A9L0RQI4; -.
DR   Ensembl; ENSECAT00000130227.1; ENSECAP00000066139.1; ENSECAG00000014979.4.
DR   GeneTree; ENSGT00950000183067; -.
DR   Proteomes; UP000002281; Chromosome 1.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   CDD; cd13306; PH1_AFAP; 1.
DR   CDD; cd13307; PH2_AFAP; 1.
DR   FunFam; 2.30.29.30:FF:000020; Actin filament-associated protein 1-like 2 isoform 1; 1.
DR   FunFam; 2.30.29.30:FF:000171; Actin filament-associated protein 1-like 2 isoform 1; 1.
DR   Gene3D; 2.30.29.30; Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB); 2.
DR   InterPro; IPR030113; AFAP.
DR   InterPro; IPR063177; AFAP_C.
DR   InterPro; IPR063153; AFAP_N.
DR   InterPro; IPR011993; PH-like_dom_sf.
DR   InterPro; IPR001849; PH_domain.
DR   PANTHER; PTHR14338; ACTIN FILAMENT-ASSOCIATED PROTEIN 1 FAMILY MEMBER; 1.
DR   PANTHER; PTHR14338:SF4; ACTIN FILAMENT-ASSOCIATED PROTEIN 1-LIKE 2; 1.
DR   Pfam; PF30810; AFAP_C; 1.
DR   Pfam; PF30812; AFAP_N; 1.
DR   Pfam; PF00169; PH; 2.
DR   SMART; SM00233; PH; 2.
DR   SUPFAM; SSF50729; PH domain-like; 2.
DR   PROSITE; PS50003; PH_DOMAIN; 2.
PE   4: Predicted;
KW   Coiled coil {ECO:0000256|ARBA:ARBA00023054};
KW   Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW   Reference proteome {ECO:0000313|Proteomes:UP000002281};
KW   Repeat {ECO:0000256|ARBA:ARBA00022737}.
FT   DOMAIN          186..282
FT                   /note="PH"
FT                   /evidence="ECO:0000259|PROSITE:PS50003"
FT   DOMAIN          364..458
FT                   /note="PH"
FT                   /evidence="ECO:0000259|PROSITE:PS50003"
FT   REGION          76..174
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          522..546
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          559..618
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          664..691
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          794..819
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        90..105
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        134..150
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        524..537
FT                   /note="Low complexity"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        681..691
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        800..815
FT                   /note="Low complexity"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   850 AA;  94944 MW;  B76997B3D5CE68E8 CRC64;
     MRSTMWVLLA NTATSNALEQ LLTELDDFLK ILDQENLSST AEVKKSGLAE LLRLYTKSSS
     SDEEYIYMNK VTVHKQQNAE SQDKVPEEQS PLTNGEPSQH TSAPQKSLPD LPPPKIIPER
     KQLSIPRIES PEGYYEEAEP YDTSLNEDGE AVSSSYESYD EEESNKGKSA PYQWPSPEAS
     IELMRDARIC AFLWRKKWLG QWAKQLCVIK DTRLLCYKSS KDHSPQLDVN LLGSSVVHKE
     KQVRKKEHKL KITPMNADVI VLGLQSKDQA EQWLRVIQEV SGLPSEGVSE GNQYTPDAQR
     PNCQKPDITE KFLSASEYGS SIEGHPEVPE TKDVKKKCSA GLKLSNLMNL GRKKSTSLEP
     PERSLETSSY LNVLVNSQWK SRWCSVRDSH LHFYQDRNRS KVAQQPLSLV GCEVVPDPSP
     DHLYSFRILH NGEELAKLEA KSSEEMGHWL GLLLSESGSK TDPEEFTYDY VDADRVSCIV
     SAAKNSLLLM QRKFSEPNTY IDGLPSQDRQ EMLYDDVEMS ELSATVEPTE EATPATDALD
     ESGPDRVYLD LTPVKSFLHS SGSAQAQAPS PPLSHLDPLA EALPADPGPS PTPDEALEMS
     PETPELQMQQ ENLESEEPSL RTTMVKIQTE QQKISFPSSC PDAVALTPAG ASPPVKDRLR
     VTTADEQTEV QRGEGTCPRS HSKEEIKLGK NRTEAEVKRY TEEKERLERK KEEIRGHLAQ
     LRKEKRELKE TLSKCTDKGV LASLEQKLKE MEEECRIEES RRVDLELNIV EVKDNLKKAE
     AGPVTLGTTV DTTHLESVSP RPKAATPTPA PDCTPVNSAT ALKNRPLSVM VTGKGTVLQK
     AKEWEKKGAS
//