id: A0A9L0R074 gene_symbol: CDK7 taxon: id: NCBITaxon:9796 label: Equus caballus status: COMPLETE description: Assessment of 2 ProtNLM GO predictions for the selected horse CDK7 protein, using mammalian experimental findings and an explicit comparison of the horse sequence. source_documents: - genes/human/CDK7/CDK7-uniprot.txt - genes/HORSE/CDK7/CDK7-bioinformatics/RESULTS.md - projects/PROTNLM_EVALUATION/mammal-benchmark/horse40-predictions.csv predictions: - source_method: ProtNLM2 source_version: UniProt API snapshot 2026-09-08 predicted_term: id: GO:0004693 label: cyclin-dependent protein serine/threonine kinase activity predicted_term_type: GO_MF review: assessment: CNN confidence_score: 2 summary: The cyclin-dependent kinase activity is supported by human CDK7 structural and biochemical work (PMID:15530371), the conserved horse catalytic Asp137 and ATP-binding residues, and 95.38% identity over the complete human reference. The horse protein has an additional segment, but the catalytic domain is retained. The exact term is already in horse GOA. Training-set membership is unknown. supported_by: - reference_id: file:human/CDK7/CDK7-uniprot.txt supporting_text: 'CC -!- FUNCTION: Serine/threonine kinase involved in cell cycle control and in CC RNA polymerase II-mediated RNA transcription (PubMed:9852112, CC PubMed:19136461, PubMed:26257281, PubMed:28768201). As a cyclin- CC dependent kinase, CDK7 is activated by the binding to cyclin-H/CCNH and CC the CDK-activating kinase assembly factor MAT1 (PubMed:41100585). CC Catalytic subunit of the CDK-activating kinase (CAK) complex, a master CC regulator of CDK activity by catalyzing the activating threonine CC phosphorylation of CDKs (PubMed:41100585). CAK activates major CC mediators of cell cycle control, including CDK1, CDK2, CDK4 and CDK6, CC and plays a key role in regulating cell cycle progression CC (PubMed:41100585). CAK complexed to the core-TFIIH basal transcription CC factor activates RNA polymerase II by serine phosphorylation of the CTD CC of POLR2A, allowing its escape from the promoter and elongation of the CC transcripts (PubMed:9852112). Initiates transcription by RNA polymerase CC II by mediating phosphorylation of POLR2A at ''Ser-5'' of the repetitive CC C-terminal domain (CTD) when POLR2A is in complex with DNA, promoting CC dissociation from DNA and initiation (PubMed:19136461, PubMed:26257281, CC PubMed:28768201). Phosphorylates SPT5/SUPT5H, SF1/NR5A1, POLR2A, CC p53/TP53, CDK1, CDK2, CDK4, CDK6 and CDK11B/CDK11 (PubMed:9372954, CC PubMed:9840937, PubMed:19136461, PubMed:26257281, PubMed:28768201, CC PubMed:41100585, PubMed:41100585). Its expression and activity are CC constant throughout the cell cycle. Upon DNA damage, triggers p53/TP53 CC activation by phosphorylation, but is inactivated in turn by p53/TP53; CC this feedback loop may lead to an arrest of the cell cycle and of the CC transcription, helping in cell recovery, or to apoptosis. Required for CC DNA-bound peptides-mediated transcription and cellular growth CC inhibition. {ECO:0000269|PubMed:10024882, ECO:0000269|PubMed:11113184, CC ECO:0000269|PubMed:16327805, ECO:0000269|PubMed:17373709, CC ECO:0000269|PubMed:17386261, ECO:0000269|PubMed:17901130, CC ECO:0000269|PubMed:19015234, ECO:0000269|PubMed:19071173, CC ECO:0000269|PubMed:19136461, ECO:0000269|PubMed:19450536, CC ECO:0000269|PubMed:19667075, ECO:0000269|PubMed:20360007, CC ECO:0000269|PubMed:26257281, ECO:0000269|PubMed:28768201, CC ECO:0000269|PubMed:41100585, ECO:0000269|PubMed:9372954, CC ECO:0000269|PubMed:9840937, ECO:0000269|PubMed:9852112}.' - reference_id: file:HORSE/CDK7/CDK7-bioinformatics/RESULTS.md supporting_text: The horse sequence A0A9L0R074 (415 residues) aligns to human P50613 (346 residues) with 95.38% identity across 346 paired residues. Paired coverage is 100.0% of the human sequence and 83.37% of the horse sequence. - reference_id: PMID:15530371 supporting_text: "CDK7, a member of the cyclin-dependent protein kinase family, regulates the \n\ activities of other CDKs through phosphorylation on their activation segment and \nhence contributes\ \ to control of the eukaryotic cell cycle. CDK7 also assists in \nthe regulation of transcription\ \ as part of the transcription factor TFIIH \ncomplex. For maximum activity and stability, CDK7\ \ requires phosphorylation, \nassociation with cyclin H, and association with a third protein,\ \ MAT1." - source_method: ProtNLM2 source_version: UniProt API snapshot 2026-09-08 predicted_term: id: GO:0006468 label: protein phosphorylation predicted_term_type: GO_BP review: assessment: COR confidence_score: 2 summary: Human CDK7 phosphorylates CDKs and the RNA polymerase II CTD; the horse catalytic domain and ATP-binding features are conserved. Protein phosphorylation is therefore a justified mammalian functional transfer. This BP term is absent from the frozen horse GOA, although kinase MF annotations are present; novelty here means target annotation coverage, not a new biochemical discovery or known absence from training. Training-set membership is unknown. supported_by: - reference_id: file:human/CDK7/CDK7-uniprot.txt supporting_text: 'CC -!- FUNCTION: Serine/threonine kinase involved in cell cycle control and in CC RNA polymerase II-mediated RNA transcription (PubMed:9852112, CC PubMed:19136461, PubMed:26257281, PubMed:28768201). As a cyclin- CC dependent kinase, CDK7 is activated by the binding to cyclin-H/CCNH and CC the CDK-activating kinase assembly factor MAT1 (PubMed:41100585). CC Catalytic subunit of the CDK-activating kinase (CAK) complex, a master CC regulator of CDK activity by catalyzing the activating threonine CC phosphorylation of CDKs (PubMed:41100585). CAK activates major CC mediators of cell cycle control, including CDK1, CDK2, CDK4 and CDK6, CC and plays a key role in regulating cell cycle progression CC (PubMed:41100585). CAK complexed to the core-TFIIH basal transcription CC factor activates RNA polymerase II by serine phosphorylation of the CTD CC of POLR2A, allowing its escape from the promoter and elongation of the CC transcripts (PubMed:9852112). Initiates transcription by RNA polymerase CC II by mediating phosphorylation of POLR2A at ''Ser-5'' of the repetitive CC C-terminal domain (CTD) when POLR2A is in complex with DNA, promoting CC dissociation from DNA and initiation (PubMed:19136461, PubMed:26257281, CC PubMed:28768201). Phosphorylates SPT5/SUPT5H, SF1/NR5A1, POLR2A, CC p53/TP53, CDK1, CDK2, CDK4, CDK6 and CDK11B/CDK11 (PubMed:9372954, CC PubMed:9840937, PubMed:19136461, PubMed:26257281, PubMed:28768201, CC PubMed:41100585, PubMed:41100585). Its expression and activity are CC constant throughout the cell cycle. Upon DNA damage, triggers p53/TP53 CC activation by phosphorylation, but is inactivated in turn by p53/TP53; CC this feedback loop may lead to an arrest of the cell cycle and of the CC transcription, helping in cell recovery, or to apoptosis. Required for CC DNA-bound peptides-mediated transcription and cellular growth CC inhibition. {ECO:0000269|PubMed:10024882, ECO:0000269|PubMed:11113184, CC ECO:0000269|PubMed:16327805, ECO:0000269|PubMed:17373709, CC ECO:0000269|PubMed:17386261, ECO:0000269|PubMed:17901130, CC ECO:0000269|PubMed:19015234, ECO:0000269|PubMed:19071173, CC ECO:0000269|PubMed:19136461, ECO:0000269|PubMed:19450536, CC ECO:0000269|PubMed:19667075, ECO:0000269|PubMed:20360007, CC ECO:0000269|PubMed:26257281, ECO:0000269|PubMed:28768201, CC ECO:0000269|PubMed:41100585, ECO:0000269|PubMed:9372954, CC ECO:0000269|PubMed:9840937, ECO:0000269|PubMed:9852112}.' - reference_id: file:HORSE/CDK7/CDK7-bioinformatics/RESULTS.md supporting_text: The horse sequence A0A9L0R074 (415 residues) aligns to human P50613 (346 residues) with 95.38% identity across 346 paired residues. Paired coverage is 100.0% of the human sequence and 83.37% of the horse sequence. - reference_id: PMID:15530371 supporting_text: "CDK7, a member of the cyclin-dependent protein kinase family, regulates the \n\ activities of other CDKs through phosphorylation on their activation segment and \nhence contributes\ \ to control of the eukaryotic cell cycle. CDK7 also assists in \nthe regulation of transcription\ \ as part of the transcription factor TFIIH \ncomplex. For maximum activity and stability, CDK7\ \ requires phosphorylation, \nassociation with cyclin H, and association with a third protein,\ \ MAT1." references: - id: file:human/CDK7/CDK7-uniprot.txt title: UniProt record for human CDK7 findings: [] - id: file:HORSE/CDK7/CDK7-bioinformatics/RESULTS.md title: "CDK7: horse\u2013human sequence comparison" findings: [] - id: PMID:15530371 title: The crystal structure of human CDK7 and its protein recognition properties. findings: []