id: A0A9L0SWY1 gene_symbol: DYNLT2B taxon: id: NCBITaxon:9796 label: Equus caballus status: COMPLETE description: The ten ProtNLM predictions match well-supported functions or localizations of intact mammalian DYNLT2B, but their transfer to this exact extended and C-terminally truncated horse protein remains unresolved. source_documents: - genes/HORSE/DYNLT2B/DYNLT2B-bioinformatics/RESULTS.md - genes/human/DYNLT2B/DYNLT2B-uniprot.txt - projects/PROTNLM_EVALUATION/mammal-benchmark/horse40-predictions.csv predictions: - source_method: ProtNLM2 source_version: UniProt API snapshot 2026-09-08 predicted_term: id: GO:0097546 label: ciliary base predicted_term_type: GO_CC review: assessment: UNC confidence_score: 1 summary: "Human ciliary-base targeting depends on microtubules and WDR60 association; conserved internal\ \ sequence does not demonstrate that the truncated horse fold can make those interactions. Global\ \ alignment covers 90.14% of the human reference but only 52.24% of the horse protein; human residues\ \ 129\u2013142 are absent, including most of the 128\u2013140 beta-strand observed in PDB 8RGI.\ \ The model-input sequence at prediction time and training membership are unknown." supported_by: - reference_id: PMID:25830415 supporting_text: Tctex1d2 associates with Wdr34, Wdr60, and other subunits of Dync1 and Dync2 and colocalizes with Wdr60 to microtubule organizing centers during interphase, the mitotic spindle poles during cell division, and the base of the cilium in ciliated cells. - reference_id: file:HORSE/DYNLT2B/DYNLT2B-bioinformatics/RESULTS.md supporting_text: "The horse sequence A0A9L0SWY1 (245 residues) aligns to human Q8WW35 (142 residues)\ \ with 91.41% identity across 128 paired residues. Paired coverage is 90.14% of the human sequence\ \ and 52.24% of the horse sequence. ... - STRAND: human [128, 129, 130, 131, 132, 133, 134, 135,\ \ 136, 137, 138, 139, 140] DSLFCVVAAFGCF \u2192 horse [245, None, None, None, None, None, None,\ \ None, None, None, None, None, None] K------------." - reference_id: file:human/DYNLT2B/DYNLT2B-uniprot.txt supporting_text: 'FT STRAND 128..140 FT /evidence="ECO:0007829|PDB:8RGI"' - source_method: ProtNLM2 source_version: UniProt API snapshot 2026-09-08 predicted_term: id: GO:0045505 label: dynein intermediate chain binding predicted_term_type: GO_MF review: assessment: UNC confidence_score: 1 summary: "Intermediate-chain binding is experimentally established for the intact human heterodimer,\ \ but the selected horse protein lacks almost the entire terminal beta-strand of that fold. A direct\ \ interaction assay or a corrected protein model is needed to transfer this molecular function.\ \ Global alignment covers 90.14% of the human reference but only 52.24% of the horse protein; human\ \ residues 129\u2013142 are absent, including most of the 128\u2013140 beta-strand observed in PDB\ \ 8RGI. The model-input sequence at prediction time and training membership are unknown." supported_by: - reference_id: PMID:29742051 supporting_text: 'Utilizing the visible immunoprecipitation assay, we demonstrated the interaction modes among the dynein-2 subunits, including previously undefined interactions, such as that between WDR60 and the TCTEX1D2-DYNLT1/DYNLT3 dimer. The dynein-2 complex can be divided into three subcomplexes, namely DYNC2H1-DYNC2LI1, WDR34-DYNLL1/DYNLL2-DYNLRB1/DYNLRB2, and WDR60-TCTEX1D2-DYNLT1/DYNLT3.' - reference_id: file:HORSE/DYNLT2B/DYNLT2B-bioinformatics/RESULTS.md supporting_text: "The horse sequence A0A9L0SWY1 (245 residues) aligns to human Q8WW35 (142 residues)\ \ with 91.41% identity across 128 paired residues. Paired coverage is 90.14% of the human sequence\ \ and 52.24% of the horse sequence. ... - STRAND: human [128, 129, 130, 131, 132, 133, 134, 135,\ \ 136, 137, 138, 139, 140] DSLFCVVAAFGCF \u2192 horse [245, None, None, None, None, None, None,\ \ None, None, None, None, None, None] K------------." - reference_id: file:human/DYNLT2B/DYNLT2B-uniprot.txt supporting_text: 'FT STRAND 128..140 FT /evidence="ECO:0007829|PDB:8RGI"' - source_method: ProtNLM2 source_version: UniProt API snapshot 2026-09-08 predicted_term: id: GO:0000922 label: spindle pole predicted_term_type: GO_CC review: assessment: UNC confidence_score: 1 summary: "The human spindle-pole observation is specific to cycling cells. Neither conservation of\ \ a partial light-chain domain nor ciliary function establishes the same localization for the extended/truncated\ \ horse protein. Global alignment covers 90.14% of the human reference but only 52.24% of the horse\ \ protein; human residues 129\u2013142 are absent, including most of the 128\u2013140 beta-strand\ \ observed in PDB 8RGI. The model-input sequence at prediction time and training membership are\ \ unknown." supported_by: - reference_id: PMID:25830415 supporting_text: Tctex1d2 associates with Wdr34, Wdr60, and other subunits of Dync1 and Dync2 and colocalizes with Wdr60 to microtubule organizing centers during interphase, the mitotic spindle poles during cell division, and the base of the cilium in ciliated cells. - reference_id: file:HORSE/DYNLT2B/DYNLT2B-bioinformatics/RESULTS.md supporting_text: "The horse sequence A0A9L0SWY1 (245 residues) aligns to human Q8WW35 (142 residues)\ \ with 91.41% identity across 128 paired residues. Paired coverage is 90.14% of the human sequence\ \ and 52.24% of the horse sequence. ... - STRAND: human [128, 129, 130, 131, 132, 133, 134, 135,\ \ 136, 137, 138, 139, 140] DSLFCVVAAFGCF \u2192 horse [245, None, None, None, None, None, None,\ \ None, None, None, None, None, None] K------------." - reference_id: file:human/DYNLT2B/DYNLT2B-uniprot.txt supporting_text: 'FT STRAND 128..140 FT /evidence="ECO:0007829|PDB:8RGI"' - source_method: ProtNLM2 source_version: UniProt API snapshot 2026-09-08 predicted_term: id: GO:0035721 label: intraciliary retrograde transport predicted_term_type: GO_BP review: assessment: UNC confidence_score: 1 summary: "Human knockout studies establish participation in retrograde IFT, but the selected horse\ \ protein has an unresolved structural truncation. This is not a claim that the normal horse ortholog\ \ lacks an evolutionarily conserved transport role. Global alignment covers 90.14% of the human\ \ reference but only 52.24% of the horse protein; human residues 129\u2013142 are absent, including\ \ most of the 128\u2013140 beta-strand observed in PDB 8RGI. The model-input sequence at prediction\ \ time and training membership are unknown." supported_by: - reference_id: PMID:29742051 supporting_text: 'We established cell lines lacking WDR60 or TCTEX1D2, both of which are dynein-2-specific subunits encoded by ciliopathy-causing genes, and found that both WDR60-knockout (KO) and TCTEX1D2-KO cells show defects in retrograde ciliary protein trafficking, with WDR60-KO cells demonstrating more severe defects probably due to failed assembly of the dynein-2 complex.' - reference_id: file:HORSE/DYNLT2B/DYNLT2B-bioinformatics/RESULTS.md supporting_text: "The horse sequence A0A9L0SWY1 (245 residues) aligns to human Q8WW35 (142 residues)\ \ with 91.41% identity across 128 paired residues. Paired coverage is 90.14% of the human sequence\ \ and 52.24% of the horse sequence. ... - STRAND: human [128, 129, 130, 131, 132, 133, 134, 135,\ \ 136, 137, 138, 139, 140] DSLFCVVAAFGCF \u2192 horse [245, None, None, None, None, None, None,\ \ None, None, None, None, None, None] K------------." - reference_id: file:human/DYNLT2B/DYNLT2B-uniprot.txt supporting_text: 'FT STRAND 128..140 FT /evidence="ECO:0007829|PDB:8RGI"' - source_method: ProtNLM2 source_version: UniProt API snapshot 2026-09-08 predicted_term: id: GO:1905799 label: regulation of intraciliary retrograde transport predicted_term_type: GO_BP review: assessment: UNC confidence_score: 1 summary: "Human loss of TCTEX1D2 perturbs IFT motor stability and transport. The selected horse model\ \ cannot yet be assumed to assemble into that regulatory complex. Global alignment covers 90.14%\ \ of the human reference but only 52.24% of the horse protein; human residues 129\u2013142 are absent,\ \ including most of the 128\u2013140 beta-strand observed in PDB 8RGI. The model-input sequence\ \ at prediction time and training membership are unknown." supported_by: - reference_id: PMID:26044572 supporting_text: 'Loss of TCTEX1D2 impairs retrograde intraflagellar transport (IFT) in humans and the protist Chlamydomonas, accompanied by destabilization of the retrograde IFT dynein motor. We thus define TCTEX1D2 as an integral component of the evolutionarily conserved retrograde IFT machinery.' - reference_id: file:HORSE/DYNLT2B/DYNLT2B-bioinformatics/RESULTS.md supporting_text: "The horse sequence A0A9L0SWY1 (245 residues) aligns to human Q8WW35 (142 residues)\ \ with 91.41% identity across 128 paired residues. Paired coverage is 90.14% of the human sequence\ \ and 52.24% of the horse sequence. ... - STRAND: human [128, 129, 130, 131, 132, 133, 134, 135,\ \ 136, 137, 138, 139, 140] DSLFCVVAAFGCF \u2192 horse [245, None, None, None, None, None, None,\ \ None, None, None, None, None, None] K------------." - reference_id: file:human/DYNLT2B/DYNLT2B-uniprot.txt supporting_text: 'FT STRAND 128..140 FT /evidence="ECO:0007829|PDB:8RGI"' - source_method: ProtNLM2 source_version: UniProt API snapshot 2026-09-08 predicted_term: id: GO:0060271 label: cilium assembly predicted_term_type: GO_BP review: assessment: UNC confidence_score: 1 summary: "Human depletion affects ciliogenesis, whereas phenotype strength varies with experimental\ \ context. Correctness for the selected horse protein depends on whether its altered termini still\ \ permit an intact light-chain fold and complex assembly. Global alignment covers 90.14% of the\ \ human reference but only 52.24% of the horse protein; human residues 129\u2013142 are absent,\ \ including most of the 128\u2013140 beta-strand observed in PDB 8RGI. The model-input sequence\ \ at prediction time and training membership are unknown." supported_by: - reference_id: PMID:25830415 supporting_text: 'Tctex1d2 and Wdr60 localize to the base of the cilium and their depletion causes defects in ciliogenesis.' - reference_id: file:HORSE/DYNLT2B/DYNLT2B-bioinformatics/RESULTS.md supporting_text: "The horse sequence A0A9L0SWY1 (245 residues) aligns to human Q8WW35 (142 residues)\ \ with 91.41% identity across 128 paired residues. Paired coverage is 90.14% of the human sequence\ \ and 52.24% of the horse sequence. ... - STRAND: human [128, 129, 130, 131, 132, 133, 134, 135,\ \ 136, 137, 138, 139, 140] DSLFCVVAAFGCF \u2192 horse [245, None, None, None, None, None, None,\ \ None, None, None, None, None, None] K------------." - reference_id: file:human/DYNLT2B/DYNLT2B-uniprot.txt supporting_text: 'FT STRAND 128..140 FT /evidence="ECO:0007829|PDB:8RGI"' - source_method: ProtNLM2 source_version: UniProt API snapshot 2026-09-08 predicted_term: id: GO:0005813 label: centrosome predicted_term_type: GO_CC review: assessment: UNC confidence_score: 1 summary: "Centrosomal localization is observed for human TCTEX1D2, but no direct localization or compensating\ \ structural evidence is available for the selected horse protein model. Global alignment covers\ \ 90.14% of the human reference but only 52.24% of the horse protein; human residues 129\u2013142\ \ are absent, including most of the 128\u2013140 beta-strand observed in PDB 8RGI. The model-input\ \ sequence at prediction time and training membership are unknown." supported_by: - reference_id: PMID:25830415 supporting_text: Tctex1d2 associates with Wdr34, Wdr60, and other subunits of Dync1 and Dync2 and colocalizes with Wdr60 to microtubule organizing centers during interphase, the mitotic spindle poles during cell division, and the base of the cilium in ciliated cells. - reference_id: file:HORSE/DYNLT2B/DYNLT2B-bioinformatics/RESULTS.md supporting_text: "The horse sequence A0A9L0SWY1 (245 residues) aligns to human Q8WW35 (142 residues)\ \ with 91.41% identity across 128 paired residues. Paired coverage is 90.14% of the human sequence\ \ and 52.24% of the horse sequence. ... - STRAND: human [128, 129, 130, 131, 132, 133, 134, 135,\ \ 136, 137, 138, 139, 140] DSLFCVVAAFGCF \u2192 horse [245, None, None, None, None, None, None,\ \ None, None, None, None, None, None] K------------." - reference_id: file:human/DYNLT2B/DYNLT2B-uniprot.txt supporting_text: 'FT STRAND 128..140 FT /evidence="ECO:0007829|PDB:8RGI"' - source_method: ProtNLM2 source_version: UniProt API snapshot 2026-09-08 predicted_term: id: GO:0005930 label: axoneme predicted_term_type: GO_CC review: assessment: UNC confidence_score: 1 summary: "The human axonemal signal came from overexpressed tagged protein; mouse sperm experiments\ \ add tissue-specific axonemal biology but are not a horse localization assay. The exact protein-model\ \ defect makes transfer additionally uncertain. Global alignment covers 90.14% of the human reference\ \ but only 52.24% of the horse protein; human residues 129\u2013142 are absent, including most of\ \ the 128\u2013140 beta-strand observed in PDB 8RGI. The model-input sequence at prediction time\ \ and training membership are unknown." supported_by: - reference_id: PMID:25830415 supporting_text: overexpression of LAP(EGFP-TEV-S-Peptide)-Tctex1d2 led to the localization of LAP-Tctex1d2 to the ciliary axoneme (Fig. 3I), similar to what had been observed with Wdr60 overexpression.4 - reference_id: file:HORSE/DYNLT2B/DYNLT2B-bioinformatics/RESULTS.md supporting_text: "The horse sequence A0A9L0SWY1 (245 residues) aligns to human Q8WW35 (142 residues)\ \ with 91.41% identity across 128 paired residues. Paired coverage is 90.14% of the human sequence\ \ and 52.24% of the horse sequence. ... - STRAND: human [128, 129, 130, 131, 132, 133, 134, 135,\ \ 136, 137, 138, 139, 140] DSLFCVVAAFGCF \u2192 horse [245, None, None, None, None, None, None,\ \ None, None, None, None, None, None] K------------." - reference_id: file:human/DYNLT2B/DYNLT2B-uniprot.txt supporting_text: 'FT STRAND 128..140 FT /evidence="ECO:0007829|PDB:8RGI"' - source_method: ProtNLM2 source_version: UniProt API snapshot 2026-09-08 predicted_term: id: GO:0031021 label: interphase microtubule organizing center predicted_term_type: GO_CC review: assessment: UNC confidence_score: 1 summary: "Human interphase microtubule-organizing-center localization is supported, but the corresponding\ \ interaction and localization of the extended/truncated horse form remain unresolved. Global alignment\ \ covers 90.14% of the human reference but only 52.24% of the horse protein; human residues 129\u2013\ 142 are absent, including most of the 128\u2013140 beta-strand observed in PDB 8RGI. The model-input\ \ sequence at prediction time and training membership are unknown." supported_by: - reference_id: PMID:25830415 supporting_text: Tctex1d2 associates with Wdr34, Wdr60, and other subunits of Dync1 and Dync2 and colocalizes with Wdr60 to microtubule organizing centers during interphase, the mitotic spindle poles during cell division, and the base of the cilium in ciliated cells. - reference_id: file:HORSE/DYNLT2B/DYNLT2B-bioinformatics/RESULTS.md supporting_text: "The horse sequence A0A9L0SWY1 (245 residues) aligns to human Q8WW35 (142 residues)\ \ with 91.41% identity across 128 paired residues. Paired coverage is 90.14% of the human sequence\ \ and 52.24% of the horse sequence. ... - STRAND: human [128, 129, 130, 131, 132, 133, 134, 135,\ \ 136, 137, 138, 139, 140] DSLFCVVAAFGCF \u2192 horse [245, None, None, None, None, None, None,\ \ None, None, None, None, None, None] K------------." - reference_id: file:human/DYNLT2B/DYNLT2B-uniprot.txt supporting_text: 'FT STRAND 128..140 FT /evidence="ECO:0007829|PDB:8RGI"' - source_method: ProtNLM2 source_version: UniProt API snapshot 2026-09-08 predicted_term: id: GO:1902017 label: regulation of cilium assembly predicted_term_type: GO_BP review: assessment: UNC confidence_score: 1 summary: "An intact dynein-2 light chain can regulate ciliary assembly/homeostasis. The selected horse\ \ sequence lacks a structured terminal segment, so its ability to exert that complex-dependent role\ \ is not established. Global alignment covers 90.14% of the human reference but only 52.24% of the\ \ horse protein; human residues 129\u2013142 are absent, including most of the 128\u2013140 beta-strand\ \ observed in PDB 8RGI. The model-input sequence at prediction time and training membership are\ \ unknown." supported_by: - reference_id: PMID:25205765 supporting_text: 'We show that the proteins encoded by the ciliopathy genes WDR34 and WDR60 are bona fide dynein-2 intermediate chains and are both required for dynein-2 function. In addition, we identify TCTEX1D2 as a unique dynein-2 light chain that is itself required for cilia function.' - reference_id: file:HORSE/DYNLT2B/DYNLT2B-bioinformatics/RESULTS.md supporting_text: "The horse sequence A0A9L0SWY1 (245 residues) aligns to human Q8WW35 (142 residues)\ \ with 91.41% identity across 128 paired residues. Paired coverage is 90.14% of the human sequence\ \ and 52.24% of the horse sequence. ... - STRAND: human [128, 129, 130, 131, 132, 133, 134, 135,\ \ 136, 137, 138, 139, 140] DSLFCVVAAFGCF \u2192 horse [245, None, None, None, None, None, None,\ \ None, None, None, None, None, None] K------------." - reference_id: file:human/DYNLT2B/DYNLT2B-uniprot.txt supporting_text: 'FT STRAND 128..140 FT /evidence="ECO:0007829|PDB:8RGI"' references: - id: file:HORSE/DYNLT2B/DYNLT2B-bioinformatics/RESULTS.md title: "DYNLT2B: horse\u2013human sequence comparison" findings: [] - id: file:human/DYNLT2B/DYNLT2B-uniprot.txt title: UniProt record for human DYNLT2B findings: [] - id: PMID:25205765 title: Subunit composition of the human cytoplasmic dynein-2 complex. findings: [] - id: PMID:25830415 title: Tctex1d2 associates with short-rib polydactyly syndrome proteins and is required for ciliogenesis. findings: [] - id: PMID:26044572 title: TCTEX1D2 mutations underlie Jeune asphyxiating thoracic dystrophy with impaired retrograde intraflagellar transport. findings: [] - id: PMID:29742051 title: Interaction of WDR60 intermediate chain with TCTEX1D2 light chain of the dynein-2 complex is crucial for ciliary protein trafficking. findings: []