id: A0A9L0SHX8
gene_symbol: MAP2K2
taxon:
  id: NCBITaxon:9796
  label: Equus caballus
status: COMPLETE
description: Assessment of 1 ProtNLM GO predictions for the selected horse MAP2K2 protein, using mammalian
  experimental findings and an explicit comparison of the horse sequence.
source_documents:
- genes/human/MAP2K2/MAP2K2-uniprot.txt
- genes/HORSE/MAP2K2/MAP2K2-bioinformatics/RESULTS.md
- projects/PROTNLM_EVALUATION/mammal-benchmark/horse40-predictions.csv
predictions:
- source_method: ProtNLM2
  source_version: UniProt API snapshot 2026-09-08
  predicted_term:
    id: GO:0006468
    label: protein phosphorylation
  predicted_term_type: GO_BP
  review:
    assessment: COR
    confidence_score: 2
    summary: MAP2K2 is a MAP kinase kinase that phosphorylates ERK-family substrates. The horse protein
      retains 97.5% of the human reference at 90.26% identity and the kinase active-site features. The
      experimentally characterized mammalian kinase mechanism supports the broad protein phosphorylation
      process. This BP term is absent from the target GOA; kinase MF overlap does not establish identical
      GO-aspect annotation or training membership. Training-set membership is unknown.
    supported_by:
    - reference_id: file:human/MAP2K2/MAP2K2-uniprot.txt
      supporting_text: 'CC   -!- FUNCTION: Catalyzes the concomitant phosphorylation of a threonine and

        CC       a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases.

        CC       Activates the ERK1 and ERK2 MAP kinases (By similarity). Activates BRAF

        CC       in a KSR1 or KSR2-dependent manner; by binding to KSR1 or KSR2 releases

        CC       the inhibitory intramolecular interaction between KSR1 or KSR2 protein

        CC       kinase and N-terminal domains which promotes KSR1 or KSR2-BRAF

        CC       dimerization and BRAF activation (PubMed:29433126).

        CC       {ECO:0000250|UniProtKB:Q63932, ECO:0000269|PubMed:29433126}.'
    - reference_id: file:HORSE/MAP2K2/MAP2K2-bioinformatics/RESULTS.md
      supporting_text: The horse sequence A0A9L0SHX8 (390 residues) aligns to human P36507 (400 residues)
        with 90.26% identity across 390 paired residues. Paired coverage is 97.5% of the human sequence
        and 100.0% of the horse sequence.
    - reference_id: PMID:8388392
      supporting_text: "Both MEK1 \nand MEK2 were expressed in Escherichia coli and shown to be able to\
        \ activate \nrecombinant human ERK1 in vitro. The purified MEK2 protein stimulated threonine \n\
        and tyrosine phosphorylation on ERK1 and concomitantly activated ERK1 kinase \nactivity more than\
        \ 100-fold."
references:
- id: file:human/MAP2K2/MAP2K2-uniprot.txt
  title: UniProt record for human MAP2K2
  findings: []
- id: file:HORSE/MAP2K2/MAP2K2-bioinformatics/RESULTS.md
  title: "MAP2K2: horse\u2013human sequence comparison"
  findings: []
- id: PMID:8388392
  title: Cloning and characterization of two distinct human extracellular signal-regulated kinase activator
    kinases, MEK1 and MEK2.
  findings: []