MYL10

Existing Annotations Review

GO Term	Evidence	Action	Reason
GO:0005509 calcium ion binding	IBA GO_REF:0000033	ACCEPT	Summary: Calcium-binding EF-hand architecture supports the conserved molecular function. Reason: Human MYL10 has PROSITE-ProRule calcium-coordinating positions in the first EF-hand. The horse protein retains the EF-hand core, including the corresponding binding-region sequence. The IBA represents curated placement of calcium binding in the light-chain phylogeny; the InterPro evidence is compatible sequence support. Direct calcium-binding measurements on horse MYL10 are not asserted. Supporting Evidence: file:human/MYL10/MYL10-uniprot.txt /ligand="Ca(2+)"
GO:0005509 calcium ion binding	IEA GO_REF:0000120	ACCEPT	Summary: Calcium-binding EF-hand architecture supports the conserved molecular function. Reason: Human MYL10 has PROSITE-ProRule calcium-coordinating positions in the first EF-hand. The horse protein retains the EF-hand core, including the corresponding binding-region sequence. The IBA represents curated placement of calcium binding in the light-chain phylogeny; the InterPro evidence is compatible sequence support. Direct calcium-binding measurements on horse MYL10 are not asserted. Supporting Evidence: file:human/MYL10/MYL10-uniprot.txt /ligand="Ca(2+)"
GO:0005737 cytoplasm	IBA GO_REF:0000033	ACCEPT	Summary: Cytoplasmic distribution is compatible with the conserved myosin light-chain role. Reason: The curated ancestral IBA supports cytoplasmic activity within the myosin light-chain family. This is an appropriate location for a soluble light-chain protein and there is no target-specific evidence of loss. It does not imply that all MYL10 is exclusively cytoplasmic. Supporting Evidence: file:human/MYL10/MYL10-uniprot.txt Myosin is a hexamer of 2 heavy chains and 4 light chains.
GO:0005739 mitochondrion	IBA GO_REF:0000033	KEEP AS NON CORE	Summary: Retain the reported mitochondrial localization as a non-core localization. Reason: Human MYL10 has a direct expressed-fusion-protein localization annotation under GO_REF:0000054. The PAINT IBA is experimentally grounded on that human observation and is not circular because human MYL10 appears as its own descendant source. The horse transfer is plausible but the physiological function of this mitochondrial pool is unresolved; do not infer a mitochondrial targeting sequence or a mitochondrial myosin motor from this observation.
GO:0005739 mitochondrion	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: Retain the reported mitochondrial localization as a non-core localization. Reason: Human MYL10 has a direct expressed-fusion-protein localization annotation under GO_REF:0000054. The PAINT IBA is experimentally grounded on that human observation and is not circular because human MYL10 appears as its own descendant source. The horse transfer is plausible but the physiological function of this mitochondrial pool is unresolved; do not infer a mitochondrial targeting sequence or a mitochondrial myosin motor from this observation.