# SIRT5 (horse): F6S899 versus alternative horse SIRT5 models A0A9L0T9B1 and A0A9L0T6A9

Generated by `compare_gene_models_rereview.py` (live UniProt and Ensembl REST; human Q9NXA8 from the cached flat file). Global alignment: Biopython PairwiseAligner, match 2, mismatch -1, gap open -10, gap extend -0.5. Raw values in `results-rereview.json`.

## Whole-length identity to human SIRT5 (Q9NXA8)

- F6S899: 282 residues; 189/280 paired residues identical (67.5%); Ensembl transcript ENSECAT00000028843 of gene ENSECAG00000026898, 10 exons.
- A0A9L0T9B1: 310 residues; 273/310 paired residues identical (88.1%); Ensembl transcript ENSECAT00000146678 of gene ENSECAG00000026898, 9 exons.
- A0A9L0T6A9: 318 residues; 254/310 paired residues identical (81.9%); Ensembl transcript ENSECAT00000079525 of gene ENSECAG00000026898, 9 exons.

## Identity by 50-residue window of human SIRT5

| human window | F6S899 | A0A9L0T9B1 | A0A9L0T6A9 |
|---|---|---|---|
| 1-50 | 40/50 | 40/50 | 40/50 |
| 51-100 | 44/50 | 44/50 | 44/50 |
| 101-150 | 42/50 | 42/50 | 42/50 |
| 151-200 | 45/50 | 45/50 | 45/50 |
| 201-250 | 10/30 | 47/50 | 47/50 |
| 251-300 | 7/40 | 47/50 | 35/50 |
| 301-310 | 1/10 | 8/10 | 1/10 |

## Human catalytic, zinc and NAD-binding features mapped onto each horse model

| feature | human pos | ligand | human | F6S899 | A0A9L0T9B1 | A0A9L0T6A9 |
|---|---|---|---|---|---|---|
| ACT_SITE | 158-158 |  | H | H | H | H |
| BINDING | 58-77 | NAD(+) | GAGVSAESGVPTFRGAGGYW | GAGISAESGVPTFRGAGGYW | GAGISAESGVPTFRGAGGYW | GAGISAESGVPTFRGAGGYW |
| BINDING | 102-102 | substrate | Y | Y | Y | Y |
| BINDING | 105-105 | substrate | R | R | R | R |
| BINDING | 140-143 | NAD(+) | QNID | QNID | QNID | QNID |
| BINDING | 166-166 | Zn(2+) | C | C | C | C |
| BINDING | 169-169 | Zn(2+) | C | C | C | C |
| BINDING | 207-207 | Zn(2+) | C | W | C | C |
| BINDING | 212-212 | Zn(2+) | C | W | C | C |
| BINDING | 249-251 | NAD(+) | GTS | -NS | GTS | GTS |
| BINDING | 275-277 | NAD(+) | NTE | --- | NME | NME |
| BINDING | 293-293 | NAD(+) | C | K | C | W |

## Exon-structure differences between the Ensembl transcripts (terminal exons include UTR, so only internal-exon mod 3 values bear on reading frame)

- Exon only in the A0A9L0T9B1 transcript: 13723059-13723160, 102 nt (length mod 3 = 0).
- Exon only in the A0A9L0T9B1 transcript: 13742662-13742785, 124 nt (length mod 3 = 1).
- Exon only in the A0A9L0T9B1 transcript: 13757807-13759730, 1924 nt (length mod 3 = 1).
- Exon only in the F6S899 transcript: 13725851-13725988, 138 nt (length mod 3 = 0).
- Exon only in the F6S899 transcript: 13726080-13726143, 64 nt (length mod 3 = 1).
- Exon only in the F6S899 transcript: 13757807-13757850, 44 nt (length mod 3 = 2).
- Exon only in the F6S899 transcript: 13757854-13757925, 72 nt (length mod 3 = 0).
- Exon in the canonical A0A9L0T6A9 transcript but not in F6S899: 13742662-13742785, 124 nt (length mod 3 = 1).
- Exon in the canonical A0A9L0T6A9 transcript but not in F6S899: 13744914-13745688, 775 nt (length mod 3 = 1).
- F6S899 transcript exons: 13725851-13725988, 13726080-13726143, 13731098-13731247, 13734819-13734952, 13737308-13737533, 13740308-13740395, 13741214-13741267, 13744914-13745029, 13757807-13757850, 13757854-13757925.
- A0A9L0T9B1 transcript exons: 13723059-13723160, 13731098-13731247, 13734819-13734952, 13737308-13737533, 13740308-13740395, 13741214-13741267, 13742662-13742785, 13744914-13745029, 13757807-13759730.
- A0A9L0T6A9 transcript exons: 13725851-13725988, 13726080-13726143, 13731098-13731247, 13734819-13734952, 13737308-13737533, 13740308-13740395, 13741214-13741267, 13742662-13742785, 13744914-13745688.

## Current GOA annotations (QuickGO) for each horse SIRT5 model

- F6S899: GO:0070403 IEA GO_REF:0000002 [InterPro:IPR003000].
- A0A9L0T9B1: GO:0008270 IEA GO_REF:0000104 [UniRule:UR000375859]; GO:0017136 IBA GO_REF:0000033 [FB:FBgn0024291, FB:FBgn0037802, FB:FBgn0038788]; GO:0034979 IEA GO_REF:0000104 [UniRule:UR000375859]; GO:0036054 IBA GO_REF:0000033 [MGI:MGI:1915596, PANTHER:PTN000119399, UniProtKB:Q9NXA8]; GO:0036054 IEA GO_REF:0000120 [InterPro:IPR027546, UniRule:UR000375859]; GO:0036055 IBA GO_REF:0000033 [MGI:MGI:1915596, PANTHER:PTN000119399, UniProtKB:Q9NXA8]; GO:0036055 IEA GO_REF:0000120 [InterPro:IPR027546, UniRule:UR000375859]; GO:0061697 IBA GO_REF:0000033 [MGI:MGI:1915596, PANTHER:PTN000119399, UniProtKB:Q9NXA8]; GO:0070403 IEA GO_REF:0000120 [InterPro:IPR003000, InterPro:IPR027546, UniRule:UR000375859]; GO:0006338 IEA GO_REF:0000108 [GO:0017136]; GO:0005634 IBA GO_REF:0000033 [AGI_LocusCode:AT5G55760, CGD:CAL0000181323, CGD:CAL0000190389]; GO:0005739 IBA GO_REF:0000033 [MGI:MGI:1915596, PANTHER:PTN000119399, UniProtKB:Q9NXA8]; GO:0005759 IBA GO_REF:0000033 [PANTHER:PTN000872307, UniProtKB:Q9NXA8]; GO:0005829 IBA GO_REF:0000033 [MGI:MGI:1915596, PANTHER:PTN000119399, UniProtKB:Q9NXA8]; GO:0005634 IEA GO_REF:0000044 [UniProtKB-SubCell:SL-0191]; GO:0005739 IEA GO_REF:0000120 [UniProtKB-SubCell:SL-0173, UniRule:UR000375859]; GO:0005829 IEA GO_REF:0000044 [UniProtKB-SubCell:SL-0091].
- A0A9L0T6A9: GO:0008270 IEA GO_REF:0000120 [UniProtKB:Q9NXA8, ensembl:ENSP00000476228, UniRule:UR000375859]; GO:0034979 IEA GO_REF:0000104 [UniRule:UR000375859]; GO:0036054 IEA GO_REF:0000120 [UniProtKB:Q8K2C6, ensembl:ENSMUSP00000152796, UniProtKB:Q9NXA8]; GO:0036055 IEA GO_REF:0000120 [UniProtKB:Q8K2C6, ensembl:ENSMUSP00000152796, UniProtKB:Q9NXA8]; GO:0061697 IEA GO_REF:0000120 [UniProtKB:Q8K2C6, ensembl:ENSMUSP00000152796, UniProtKB:Q9NXA8]; GO:0070403 IEA GO_REF:0000120 [UniProtKB:Q9NXA8, ensembl:ENSP00000476228, InterPro:IPR003000]; GO:0010566 IEA GO_REF:0000107 [UniProtKB:Q8K2C6, ensembl:ENSMUSP00000152796]; GO:2000378 IEA GO_REF:0000107 [UniProtKB:Q9NXA8, ensembl:ENSP00000476228]; GO:0005634 IEA GO_REF:0000044 [UniProtKB-SubCell:SL-0191]; GO:0005739 IEA GO_REF:0000120 [UniProtKB:Q8K2C6, ensembl:ENSMUSP00000152796, UniProtKB:Q9NXA8]; GO:0005758 IEA GO_REF:0000107 [UniProtKB:Q9NXA8, ensembl:ENSP00000476228]; GO:0005759 IEA GO_REF:0000107 [UniProtKB:Q9NXA8, ensembl:ENSP00000476228]; GO:0005829 IEA GO_REF:0000120 [UniProtKB:Q8K2C6, ensembl:ENSMUSP00000152796, UniProtKB:Q9NXA8]; GO:0005743 IEA GO_REF:0000107 [UniProtKB:Q8K2C6, ensembl:ENSMUSP00000152796].