# USP8 horse gene models vs human P40818 (re-review)

All four horse UniProt entries below are products of Ensembl gene ENSECAG00000006611 (USP8). Benchmark target is A0A9L0T7K6. Human P40818 isoform 1 is 1118 aa. Global alignment: Biopython PairwiseAligner, match 2, mismatch -1, gap open -10, gap extend -0.5.

## A0A9L0T7K6 (benchmark target)

- A0A9L0T7K6: 1112 aa; 91.82% identity over 1112 paired residues; 99.46% of human covered.
- Gaps: human 632-632 absent from horse; human 1102-1106 absent from horse
- MIT 33-116: 84/84 residues aligned
- rhodanese 195-313: 119/119 residues aligned
- SH3-binding 405-413: 9/9 residues aligned
- isoform-2-absent 601-629: 29/29 residues aligned
- USP 777-1109: 328/333 residues aligned
- Sites: human S718 -> horse S717, human C786 -> horse C785, human H1067 -> horse H1066
- C-terminus from human 1090: human DISVSSVKSSAAYILFYTSLGPRVTDVAT; horse DIAVSSVKSSAASFSLHFREISQS

## F7BV54

- F7BV54: 1117 aa; 92.75% identity over 1117 paired residues; 99.91% of human covered.
- Gaps: human 632-632 absent from horse
- MIT 33-116: 84/84 residues aligned
- rhodanese 195-313: 119/119 residues aligned
- SH3-binding 405-413: 9/9 residues aligned
- isoform-2-absent 601-629: 29/29 residues aligned
- USP 777-1109: 333/333 residues aligned
- Sites: human S718 -> horse S717, human C786 -> horse C785, human H1067 -> horse H1066
- C-terminus from human 1090: human DISVSSVKSSAAYILFYTSLGPRVTDVAT; horse DIAVSSVKSSAAYILFYTSLGPRAIDVAT

## A0A3Q2HXC7

- A0A3Q2HXC7: 1139 aa; 92.75% identity over 1117 paired residues; 99.91% of human covered.
- Gaps: horse 84-105 inserted (no human counterpart); human 632-632 absent from horse
- MIT 33-116: 84/84 residues aligned
- rhodanese 195-313: 119/119 residues aligned
- SH3-binding 405-413: 9/9 residues aligned
- isoform-2-absent 601-629: 29/29 residues aligned
- USP 777-1109: 333/333 residues aligned
- Sites: human S718 -> horse S739, human C786 -> horse C807, human H1067 -> horse H1088
- C-terminus from human 1090: human DISVSSVKSSAAYILFYTSLGPRVTDVAT; horse DIAVSSVKSSAAYILFYTSLGPRAIDVAT

## A0A5F5PIL5

- A0A5F5PIL5: 1011 aa; 92.88% identity over 1011 paired residues; 90.43% of human covered.
- Gaps: human 35-111 absent from horse; human 602-631 absent from horse
- MIT 33-116: 7/84 residues aligned
- rhodanese 195-313: 119/119 residues aligned
- SH3-binding 405-413: 9/9 residues aligned
- isoform-2-absent 601-629: 1/29 residues aligned
- USP 777-1109: 333/333 residues aligned
- Sites: human S718 -> horse S611, human C786 -> horse C679, human H1067 -> horse H960
- C-terminus from human 1090: human DISVSSVKSSAAYILFYTSLGPRVTDVAT; horse DIAVSSVKSSAAYILFYTSLGPRAIDVAT