id: A0A3Q2KRK8 gene_symbol: WDPCP taxon: id: NCBITaxon:9796 label: Equus caballus status: COMPLETE description: Broad ciliary and cytoskeletal roles are supported by mammalian experiments and the conserved horse scaffold. source_documents: - projects/PROTNLM_EVALUATION/mammal-benchmark/horse40-predictions.csv - projects/PROTNLM_EVALUATION/mammal-benchmark/predictions.jsonl.gz - projects/PROTNLM_EVALUATION/mammal-benchmark/paired-sequences/WDPCP.json - genes/HORSE/WDPCP/WDPCP-hypotheses/horse40-cell-projection-organization/openscientist.md references: - id: PMID:24302887 title: Wdpcp, a PCP protein required for ciliogenesis, regulates directional cell migration and cell polarity by direct modulation of the actin cytoskeleton. full_text_unavailable: false findings: [] - id: file:HORSE/WDPCP/WDPCP-bioinformatics/RESULTS.md title: HORSE/WDPCP/WDPCP-bioinformatics/RESULTS.md findings: [] - id: PMID:35427153 title: Structure of the ciliogenesis-associated CPLANE complex. full_text_unavailable: false findings: [] - id: file:HORSE/WDPCP/WDPCP-hypotheses/horse40-cell-projection-organization/openscientist.md title: 'OpenScientist hypothesis run: WDPCP cell projection organization' publication_type: DEEP_RESEARCH reference_review: relevance: HIGH review_notes: Substantive computational synthesis directly addressing the GO:0030030 prediction. The report's broad WDPCP/CPLANE orthology conclusion is independently corroborated by the cached sequence-comparison evidence, and its orthology-only and term-specificity caveats were retained. findings: - statement: OpenScientist supported GO:0030030 as a broad horse WDPCP process annotation via conserved CPLANE/ciliogenesis and planar-polarity biology, with curation caveats on term breadth and orthology-only evidence. supporting_text: GO:0030030 is therefore biologically justified for the horse protein. The two curation caveats are that the term is generic (specific children such as cilium assembly and establishment of planar polarity are better supported) and that all functional evidence is transferred by orthology to an unreviewed TrEMBL record, so an **ISS/ISO** evidence code is appropriate rather than an experimental one. predictions: - source_method: ProtNLM2 source_version: UniProt API snapshot 2026-09-08 predicted_term: id: GO:0030030 label: cell projection organization predicted_term_type: GO_BP review: assessment: COR confidence_score: 2 summary: Wdpcp loss disrupts cilia and actin-based protrusive behavior. Conservation of the WD40/alpha-solenoid scaffold supports the broad cell-projection-organization prediction; the OpenScientist 83% identity estimate and cached 88.1% identity over 704 paired residues reflect different alignment scopes but the same WDPCP orthology. The N-terminal length difference resembles known human–mouse variation; a short lipid-binding-tail deletion leaves affinity, not this entire broad role, unresolved. More specific children such as cilium assembly and establishment of planar polarity are better supported, and any horse assignment should use ISS/ISO rather than experimental evidence. supported_by: - &id003 reference_id: PMID:24302887 supporting_text: Wdpcp is localized to the transition zone - reference_id: PMID:24302887 supporting_text: 'decreased membrane ruffling, failure to establish cell polarity, and loss of directional cell migration.' - &id001 reference_id: file:HORSE/WDPCP/WDPCP-bioinformatics/RESULTS.md supporting_text: share 88.1% identity among 704 paired residues. - reference_id: PMID:35427153 supporting_text: Compared to mouse, the human Wdpcp has an N-terminal extension of ~35 residues that is not resolved in the map. - &id002 reference_id: file:HORSE/WDPCP/WDPCP-bioinformatics/RESULTS.md supporting_text: The WD40/alpha-solenoid scaffold is extensively conserved, supporting broad ciliary and cytoskeletal roles. - reference_id: file:HORSE/WDPCP/WDPCP-hypotheses/horse40-cell-projection-organization/openscientist.md supporting_text: GO:0030030 is therefore biologically justified for the horse protein. The two curation caveats are that the term is generic (specific children such as cilium assembly and establishment of planar polarity are better supported) and that all functional evidence is transferred by orthology to an unreviewed TrEMBL record, so an **ISS/ISO** evidence code is appropriate rather than an experimental one. - source_method: ProtNLM2 source_version: UniProt API snapshot 2026-09-08 predicted_term: id: GO:0005737 label: cytoplasm predicted_term_type: GO_CC review: assessment: COR confidence_score: 2 summary: Mouse imaging directly detects a cytoplasmic WDPCP pool on actin and focal adhesions. The conserved horse architecture supports this localization independently of electronic annotation agreement. supported_by: - &id004 reference_id: PMID:24302887 supporting_text: 'Wdpcp is also found in the cytoplasm, where it is localized in the actin cytoskeleton and in focal adhesions.' - *id001 - *id002 - source_method: ProtNLM2 source_version: UniProt API snapshot 2026-09-08 predicted_term: id: GO:0005929 label: cilium predicted_term_type: GO_CC review: assessment: COR confidence_score: 2 summary: Transition-zone localization places WDPCP at the ciliary base. Conserved horse scaffold architecture supports this broad cilium location; it does not establish axonemal shaft localization. supported_by: - *id003 - *id001 - *id002 - source_method: ProtNLM2 source_version: UniProt API snapshot 2026-09-08 predicted_term: id: GO:0005856 label: cytoskeleton predicted_term_type: GO_CC review: assessment: COR confidence_score: 2 summary: Wdpcp localizes to the actin cytoskeleton and recruits Sept2. The conserved horse scaffold supports a cytoskeletal pool, without requiring it to be an actin motor or a tubulin polymerization enzyme. supported_by: - *id004 - reference_id: PMID:24302887 supporting_text: 'in Wdpcp-deficient cells, Sept2 was lost from the actin cytoskeleton, suggesting Wdpcp is required for Sept2 recruitment to actin filaments.' - *id001 - *id002