A0A804UIX9 is an uncharacterized 375-amino acid protein from Zea mays (maize, cultivar B73) encoded on chromosome 9 (gene model Zm00001eb381380). It is classified as a PGG domain-containing protein based on the presence of two PGG repeats (Pfam PF13962 / InterPro IPR026961) spanning residues 50-157 and 217-294. The PGG domain is named for a conserved Pro-Gly-Gly motif and is relatively uncharacterized; no enzymatic activity or specific molecular function has been experimentally demonstrated for PGG domain proteins. The protein is predicted by Phobius to contain six transmembrane helices (at approximately residues 54-73, 102-123, 135-154, 166-189, 222-239, and 269-290), indicating it is a multi-pass integral membrane protein. The N-terminal region (residues 1-44) is predicted to be disordered with a polar residue compositional bias (residues 9-26). PANTHER classifies this protein in the CASKIN family (PTHR24177), subfamily SF432. No experimental data exist for this protein (UniProt protein evidence level 4: Predicted), no gene name has been assigned, and its biological function remains unknown. The deep research report for this gene incorrectly interpreted it as a polygalacturonase-inhibiting protein (PGIP); PGIPs are extracellular LRR proteins that lack transmembrane domains, which is incompatible with the six predicted transmembrane helices in A0A804UIX9.
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Download this section (compressed HTML)Q: What is the molecular function of the PGG domain (PF13962/IPR026961)? This domain is found in multi-pass transmembrane proteins but has no experimentally characterized function. Does it mediate protein-protein interactions, transport, or signaling?
Q: What is the relationship between plant PGG domain-containing transmembrane proteins and the CASKIN family (PANTHER PTHR24177)? The CASKIN classification seems unexpected for a plant membrane protein.
Q: Is A0A804UIX9 expressed in specific maize tissues or developmental stages? Expression data from RNA-seq atlases (e.g., ExpressionAtlas) could provide clues about biological function.
Experiment: Express GFP-tagged A0A804UIX9 in maize protoplasts or Nicotiana benthamiana leaf epidermal cells and examine subcellular localization by confocal microscopy, co-staining with organelle markers (ER, Golgi, plasma membrane, tonoplast).
Hypothesis: A0A804UIX9 is an integral membrane protein localized to a specific endomembrane compartment.
Type: fluorescence microscopy
Experiment: Generate CRISPR/Cas9 knockout lines for Zm00001eb381380 in maize B73. Phenotype under normal growth conditions and under biotic (fungal infection) and abiotic (drought, salt) stress conditions.
Hypothesis: A0A804UIX9 has a role in maize development or stress response.
Type: reverse genetics
These computational predictions are reviewed separately from the GOA annotation set used for this review. The assessments below are from this project and do not constitute official GO annotations or endorsement by GO/UniProt. They are not included in the existing annotation review above.
View prediction review YAML · A0A804UIX9-protnlm-predictions-review.yaml · Review status: COMPLETE
ProtNLM2 made no predictions (GO or subcellular location) for PGG domain-containing protein. The model returned only a protein name.
Source documents: genes/MAIZE/A0A804UIX9/A0A804UIX9-uniprot.txt · genes/MAIZE/A0A804UIX9/A0A804UIX9-goa.tsv
No GO/EC predictions in the reviewed source.
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