Adh1

UniProt ID: H9BHC8
Organism: Miscanthus sinensis
Review Status: INITIALIZED
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Gene Description

Cytosolic NAD-dependent alcohol dehydrogenase 1 fragment, a zinc-binding homodimeric enzyme that oxidizes primary and secondary alcohols.

Core Functions

Catalyzes NAD-dependent oxidation of alcohols in the cytosol.

Molecular Function:
alcohol dehydrogenase (NAD+) activity
Directly Involved In:
alcohol metabolic process
Cellular Locations:
cytosol
Supporting Evidence:
  • file:MISSI/Adh1/Adh1-uniprot.txt
    Reaction=a primary alcohol + NAD(+) = an aldehyde + NADH + H(+)
  • file:MISSI/Adh1/Adh1-uniprot.txt
    Reaction=a secondary alcohol + NAD(+) = a ketone + NADH + H(+)
  • file:MISSI/Adh1/Adh1-uniprot.txt
    GO; GO:0004022; F:alcohol dehydrogenase (NAD+) activity
  • file:MISSI/Adh1/Adh1-uniprot.txt
    SUBCELLULAR LOCATION: Cytoplasm

References

file:MISSI/Adh1/Adh1-uniprot.txt
UniProtKB entry for Miscanthus sinensis Adh1
  • UniProt identifies Adh1 as alcohol dehydrogenase 1 with EC 1.1.1.1, NAD-dependent alcohol oxidation reactions, zinc binding, homodimerization, cytoplasmic localization, and an alcohol dehydrogenase GO cross-reference.
file:interpro/panther/PTHR43880/PTHR43880-entries.csv
PANTHER PTHR43880 family entries for alcohol dehydrogenase-related proteins
  • The PANTHER family contains plant ADH1 members as well as related alcohol dehydrogenase classes, so the broad NAD-dependent alcohol dehydrogenase activity is used rather than class-3 formaldehyde-specific predictions.

Suggested Questions for Experts

Q: Does Miscanthus Adh1 contribute to anaerobic or stress-associated alcohol metabolism in specific tissues?

Suggested Experiments

Experiment: Measure NAD-dependent alcohol dehydrogenase activity and fermentation metabolites in Miscanthus tissues under low-oxygen stress after Adh1 perturbation.

Type: targeted functional assay

📚 Additional Documentation

Notes

(Adh1-notes.md)

Adh1 notes

  • The GOA file is header-only, so there are no existing GOA annotations to review or convert into NEW rows.
  • UniProt identifies this as alcohol dehydrogenase 1, EC 1.1.1.1, with NAD-dependent primary- and secondary-alcohol oxidation reactions [file:MISSI/Adh1/Adh1-uniprot.txt "RecName: Full=Alcohol dehydrogenase 1"] [file:MISSI/Adh1/Adh1-uniprot.txt "Reaction=a primary alcohol + NAD(+) = an aldehyde + NADH + H(+)"].
  • The core function uses alcohol dehydrogenase (NAD+) activity and alcohol metabolic process; cytosol is supported by the UniProt subcellular location and GO cross-reference [file:MISSI/Adh1/Adh1-uniprot.txt "GO; GO:0004022; F:alcohol dehydrogenase (NAD+) activity"] [file:MISSI/Adh1/Adh1-uniprot.txt "SUBCELLULAR LOCATION: Cytoplasm"].
  • I did not propagate the TreeGrafter S-(hydroxymethyl)glutathione dehydrogenase activity or formaldehyde catabolic process annotations because the PANTHER family contains multiple ADH classes and the Miscanthus entry is specifically named ADH1 with generic EC 1.1.1.1 alcohol dehydrogenase reactions [file:interpro/panther/PTHR43880/PTHR43880-entries.csv "P00333,Alcohol dehydrogenase 1"].

📄 View Raw YAML

 
id: H9BHC8
gene_symbol: Adh1
product_type: PROTEIN
status: INITIALIZED
taxon:
  id: NCBITaxon:62337
  label: Miscanthus sinensis
description: Cytosolic NAD-dependent alcohol dehydrogenase 1 fragment, a zinc-binding homodimeric enzyme that oxidizes primary and secondary alcohols.
existing_annotations: []
references:
- id: file:MISSI/Adh1/Adh1-uniprot.txt
  title: UniProtKB entry for Miscanthus sinensis Adh1
  findings:
  - statement: UniProt identifies Adh1 as alcohol dehydrogenase 1 with EC 1.1.1.1, NAD-dependent alcohol oxidation reactions, zinc binding, homodimerization, cytoplasmic localization, and an alcohol dehydrogenase GO cross-reference.
- id: file:interpro/panther/PTHR43880/PTHR43880-entries.csv
  title: PANTHER PTHR43880 family entries for alcohol dehydrogenase-related proteins
  findings:
  - statement: The PANTHER family contains plant ADH1 members as well as related alcohol dehydrogenase classes, so the broad NAD-dependent alcohol dehydrogenase activity is used rather than class-3 formaldehyde-specific predictions.
core_functions:
- description: Catalyzes NAD-dependent oxidation of alcohols in the cytosol.
  molecular_function:
    id: GO:0004022
    label: alcohol dehydrogenase (NAD+) activity
  directly_involved_in:
  - id: GO:0006066
    label: alcohol metabolic process
  locations:
  - id: GO:0005829
    label: cytosol
  supported_by:
  - reference_id: file:MISSI/Adh1/Adh1-uniprot.txt
    supporting_text: Reaction=a primary alcohol + NAD(+) = an aldehyde + NADH + H(+)
  - reference_id: file:MISSI/Adh1/Adh1-uniprot.txt
    supporting_text: Reaction=a secondary alcohol + NAD(+) = a ketone + NADH + H(+)
  - reference_id: file:MISSI/Adh1/Adh1-uniprot.txt
    supporting_text: 'GO; GO:0004022; F:alcohol dehydrogenase (NAD+) activity'
  - reference_id: file:MISSI/Adh1/Adh1-uniprot.txt
    supporting_text: 'SUBCELLULAR LOCATION: Cytoplasm'
proposed_new_terms: []
suggested_questions:
- question: Does Miscanthus Adh1 contribute to anaerobic or stress-associated alcohol metabolism in specific tissues?
suggested_experiments:
- description: Measure NAD-dependent alcohol dehydrogenase activity and fermentation metabolites in Miscanthus tissues under low-oxygen stress after Adh1 perturbation.
  experiment_type: targeted functional assay