MnSOD

UniProt ID: A0A088MDT8
Organism: Miscanthus sinensis
Review Status: INITIALIZED
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Gene Description

Mitochondrial manganese superoxide dismutase that detoxifies superoxide radicals in the mitochondrial matrix by converting superoxide to hydrogen peroxide and oxygen.

Existing Annotations Review

GO Term Evidence Action Reason
GO:0004784 superoxide dismutase activity
IEA
GO_REF:0000120 		ACCEPT
Summary: The EC/RHEA-supported UniProt annotation matches the protein name, catalytic reaction, and MnSOD family assignment.
Reason: Superoxide dismutase activity is the defining catalytic activity of MnSOD and is directly supported by the UniProt EC 1.15.1.1 reaction.
Supporting Evidence:
file:MISSI/MnSOD/MnSOD-uniprot.txt
Reaction=2 superoxide + 2 H(+) = H2O2 + O2
GO:0005739 mitochondrion
IEA
GO_REF:0000118 		KEEP AS NON CORE
Summary: Mitochondrial localization is supported, but the matrix annotation is more specific for this MnSOD entry.
Reason: The broader mitochondrion term is true but less informative than the mitochondrial matrix annotation.
Supporting Evidence:
file:MISSI/MnSOD/MnSOD-uniprot.txt
SUBCELLULAR LOCATION: Mitochondrion matrix
GO:0005759 mitochondrial matrix
IEA
GO_REF:0000044 		ACCEPT
Summary: UniProt subcellular-location annotation places the protein in the mitochondrial matrix.
Reason: The matrix is the specific mitochondrial compartment supported by the UniProt entry.
Supporting Evidence:
file:MISSI/MnSOD/MnSOD-uniprot.txt
SUBCELLULAR LOCATION: Mitochondrion matrix
GO:0006801 superoxide metabolic process
IEA
GO_REF:0000002 		KEEP AS NON CORE
Summary: Superoxide metabolism is supported by the SOD reaction, although the specific removal-of-superoxide-radicals annotation captures the core process more precisely.
Reason: This broader process term is correct but less specific than GO:0019430 for MnSOD's core role.
Supporting Evidence:
file:MISSI/MnSOD/MnSOD-uniprot.txt
Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
GO:0006950 response to stress
IEA
GO_REF:0000117 		KEEP AS NON CORE
Summary: Oxidative radical detoxification contributes to stress response, but this term is broad and not the core biochemical function.
Reason: Stress response is a downstream biological context for MnSOD activity rather than its defining molecular role.
Supporting Evidence:
file:MISSI/MnSOD/MnSOD-uniprot.txt
Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
GO:0019430 removal of superoxide radicals
IEA
GO_REF:0000108 		ACCEPT
Summary: The GO term matches the catalytic SOD reaction converting superoxide radicals to hydrogen peroxide and oxygen.
Reason: Removal of superoxide radicals is the direct biological process accomplished by MnSOD activity.
Supporting Evidence:
file:MISSI/MnSOD/MnSOD-uniprot.txt
Reaction=2 superoxide + 2 H(+) = H2O2 + O2
GO:0030145 manganese ion binding
IEA
GO_REF:0000118 		ACCEPT
Summary: Mn(2+) cofactor binding is explicitly recorded in UniProt and is consistent with a manganese superoxide dismutase.
Reason: Manganese binding is the specific metal cofactor association for MnSOD catalysis.
Supporting Evidence:
file:MISSI/MnSOD/MnSOD-uniprot.txt
COFACTOR: Name=Mn(2+)
GO:0046872 metal ion binding
IEA
GO_REF:0000002 		KEEP AS NON CORE
Summary: Generic metal ion binding is true through the manganese cofactor but less specific than GO:0030145.
Reason: The specific manganese ion binding annotation should represent the core cofactor-binding aspect; the parent metal-binding term is non-core.

Core Functions

Catalyzes manganese-dependent dismutation of mitochondrial superoxide radicals to hydrogen peroxide and oxygen.

Molecular Function:
superoxide dismutase activity
Directly Involved In:
removal of superoxide radicals
Cellular Locations:
mitochondrial matrix
Supporting Evidence:
  • file:MISSI/MnSOD/MnSOD-uniprot.txt
    Reaction=2 superoxide + 2 H(+) = H2O2 + O2
  • file:MISSI/MnSOD/MnSOD-uniprot.txt
    COFACTOR: Name=Mn(2+)
  • file:MISSI/MnSOD/MnSOD-uniprot.txt
    SUBCELLULAR LOCATION: Mitochondrion matrix

References

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Suggested Questions for Experts

Q: Is Miscanthus MnSOD activity dynamically regulated during drought, cold, or high-light oxidative stress?

Suggested Experiments

Experiment: Measure mitochondrial SOD activity and ROS accumulation in Miscanthus stress treatments.

Type: targeted functional assay

📚 Additional Documentation

Notes

(MnSOD-notes.md)

Curation notes

  • GOA contains 8 IEA annotations for MnSOD; all were reviewed rather than treating them as new annotations.
  • Superoxide dismutase activity, removal of superoxide radicals, mitochondrial matrix localization, and manganese ion binding are accepted as core-supported annotations.
  • Broader parent/context terms (mitochondrion, superoxide metabolic process, response to stress, metal ion binding) are retained as non-core because more specific annotations capture the core biology.
  • Added the UniProt-cited transcript-level PMID:26334392 reference after PR review suggested making the Miscanthus-specific cloning evidence explicit.

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