Chloroplast thylakoid membrane PsbN protein, probably involved in photosystem biogenesis rather than a stable photosystem II core subunit.
Q: Does Miscanthus psbN affect PSI or PSII assembly intermediates without being a stable photosystem subunit?
Experiment: Analyze PSI and PSII assembly intermediates and mature complex abundance after psbN perturbation.
Type: targeted functional assay
The GOA file for psbN contains only the header row, so there are no existing GOA annotations to review.
UniProt cautions that PsbN was originally thought to be a PSII component but probably is not. The review therefore captures a photosystem biogenesis role and avoids placing PsbN in the PSII complex.
id: A0A0S2RMA5
gene_symbol: psbN
product_type: PROTEIN
status: INITIALIZED
taxon:
id: NCBITaxon:62337
label: Miscanthus sinensis
description: Chloroplast thylakoid membrane PsbN protein, probably involved in photosystem biogenesis rather than a stable photosystem II core subunit.
existing_annotations: []
references:
- id: file:MISSI/psbN/psbN-uniprot.txt
title: UniProtKB entry for Miscanthus sinensis psbN
findings:
- statement: UniProt describes PsbN as a thylakoid membrane protein that may play a role in photosystem I and II biogenesis and cautions that it is probably not a PSII component.
core_functions:
- description: Supports photosystem biogenesis at the thylakoid membrane, with evidence favoring a biogenesis role rather than membership in the PSII core complex.
directly_involved_in:
- id: GO:0048564
label: photosystem I assembly
- id: GO:0010207
label: photosystem II assembly
locations:
- id: GO:0009535
label: chloroplast thylakoid membrane
supported_by:
- reference_id: file:MISSI/psbN/psbN-uniprot.txt
supporting_text: May play a role in photosystem I and II biogenesis.
- reference_id: file:MISSI/psbN/psbN-uniprot.txt
supporting_text: 'SUBCELLULAR LOCATION: Cellular thylakoid membrane'
- reference_id: file:MISSI/psbN/psbN-uniprot.txt
supporting_text: Originally thought to be a component of PSII; based on experiments in Synechocystis, N.tabacum and barley, and its absence from PSII in T.elongatus and T.vulcanus, this is probably not true.
proposed_new_terms: []
suggested_questions:
- question: Does Miscanthus psbN affect PSI or PSII assembly intermediates without being a stable photosystem subunit?
suggested_experiments:
- description: Analyze PSI and PSII assembly intermediates and mature complex abundance after psbN perturbation.
experiment_type: targeted functional assay