A conserved hypothetical protein in Mycobacterium tuberculosis H37Rv that is essential for growth in minimal media and during infection. An automated PROSITE intein signature (PS00881) match prompted a predicted intein / autoproteolytic peptidase function, but structural and sequence analysis refutes this: the protein has no intein catalytic residues, no intein domain in any database, no intein-like fold, and is a standalone gene rather than an intervening sequence embedded in a host protein. Localizes to the cell envelope and is exported via a signal peptide. Required for bacterial virulence, particularly in central nervous system infections. Despite being well-conserved across mycobacteria, its precise molecular function remains unknown.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0030313 cell envelope | IEA | NEW | Summary: Rv0311 localizes to the cell envelope and is exported via a signal peptide, as detected in multiple cellular fractions. Reason: This cellular component term reflects Rv0311's subcellular localization to the cell envelope, supported by both sequence analysis and proteomics data. Supporting Evidence: file:MYCTU/Rv0311/Rv0311-deep-research.md Rv0311 localizes to the cell envelope and is exported via a signal peptide |
| GO:0040007 growth | IEA | NEW | Summary: Rv0311 is essential for mycobacterial growth under nutrient-limited conditions, particularly in minimal media. Reason: This biological process term reflects Rv0311's essential role in bacterial growth under specific conditions, supported by essentiality studies. Supporting Evidence: file:MYCTU/Rv0311/Rv0311-deep-research.md Rv0311 is essential for growth in minimal media but not in rich media, as demonstrated by transposon mutagenesis studies |
| GO:0075001 adhesion of symbiont infection structure to host | IEA | NEW | Summary: Rv0311 is required for virulence and bacterial survival within the host, particularly during CNS infections. Reason: This biological process term reflects Rv0311's role in pathogenesis and host infection, supported by virulence studies showing attenuation in mutants. Supporting Evidence: file:MYCTU/Rv0311/Rv0311-deep-research.md Rv0311 is required for virulence particularly in CNS infections, with insertion mutants significantly attenuated in brain infection models |
| GO:0005515 protein binding | NAS | NEW | Summary: Added to align core_functions with existing annotations. Reason: Core function term not present in existing_annotations. |
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