id: A0A8B6BFL6
gene_symbol: A0A8B6BFL6
taxon:
  id: NCBITaxon:29158
  label: Mytilus galloprovincialis
status: COMPLETE
description: >-
  The reverse-transcriptase domain supports broad DNA interaction. Recombination and integration require
  evidence for the mobile element and this protein's role within it.
source_documents:
  - genes/MYTGA/A0A8B6BFL6/A0A8B6BFL6-uniprot.txt
  - genes/MYTGA/A0A8B6BFL6/A0A8B6BFL6-goa.tsv
  - publications/PMID_32170321.md
predictions:
  - source_method: ProtNLM2
    source_version: UniProt 2024_06 pilot
    predicted_term:
      id: GO:0003677
      label: DNA binding
    predicted_term_type: GO_MF
    review:
      assessment: COR
      confidence_score: 2
      summary: >-
        The target contains a reverse-transcriptase domain at residues 174–349 and a DNA/RNA polymerase
        fold assignment. Reverse transcription entails interaction with a DNA primer or nascent DNA product,
        providing a mechanistic basis for broad DNA binding. This inference does not specify a recognition
        sequence, chromosomal target, or recombinase activity. The cached GOA/UniProt record has no DNA-binding
        annotation, so this is a supported additional function.
      supported_by:
        - reference_id: file:MYTGA/A0A8B6BFL6/A0A8B6BFL6-uniprot.txt
          supporting_text: >-
            ID   A0A8B6BFL6_MYTGA        Unreviewed;       612 AA. ... FT   DOMAIN          174..349 ...
            FT                   /note="Reverse transcriptase"
  - source_method: ProtNLM2
    source_version: UniProt 2024_06 pilot
    predicted_term:
      id: GO:0006310
      label: DNA recombination
    predicted_term_type: GO_BP
    review:
      assessment: UNC
      confidence_score: 1
      summary: >-
        The sequence supports a reverse-transcriptase relationship but has no identified recombinase domain
        or documented complete mobile-element context. Experimental DIRS retrotransposition work distinguishes
        cDNA production from completion of an element's integration cycle (PMID:32170321); it does not
        establish that this mussel protein belongs to that element class. An RT can participate in a larger
        recombination pathway, so lack of its own recombinase domain is not a decisive refutation of the
        biological process. The process is absent from the cached annotations and requires verified element
        context or functional evidence.
      supported_by:
        - reference_id: file:MYTGA/A0A8B6BFL6/A0A8B6BFL6-uniprot.txt
          supporting_text: >-
            ID   A0A8B6BFL6_MYTGA        Unreviewed;       612 AA. ... FT   DOMAIN          174..349 ...
            FT                   /note="Reverse transcriptase"
        - reference_id: PMID:32170321
          supporting_text: >-
            DIRS-1 produces a mixture of single-stranded, mostly linear extrachromosomal cDNA intermediates
  - source_method: ProtNLM2
    source_version: UniProt 2024_06 pilot
    predicted_term:
      id: GO:0015074
      label: DNA integration
    predicted_term_type: GO_BP
    review:
      assessment: UNC
      confidence_score: 1
      summary: >-
        A reverse-transcriptase domain establishes a plausible role in cDNA synthesis, not necessarily
        chromosomal integration. DIRS experiments demonstrate that productive retrotransposition depends
        on the element and its proteins together (PMID:32170321), but the target's element identity and
        integration machinery are not established in the inspected sources. The prediction is a biological
        process, so a protein could contribute without catalyzing integration itself. DNA integration
        is absent from the cached annotations and remains uncertain pending element-context evidence.
      supported_by:
        - reference_id: file:MYTGA/A0A8B6BFL6/A0A8B6BFL6-uniprot.txt
          supporting_text: >-
            ID   A0A8B6BFL6_MYTGA        Unreviewed;       612 AA. ... FT   DOMAIN          174..349 ...
            FT                   /note="Reverse transcriptase"
        - reference_id: PMID:32170321
          supporting_text: >-
            DIRS-1 produces a mixture of single-stranded, mostly linear extrachromosomal cDNA intermediates
references:
  - id: file:MYTGA/A0A8B6BFL6/A0A8B6BFL6-uniprot.txt
    title: A0A8B6BFL6-uniprot.txt
  - id: PMID:32170321
    title: DIRS retrotransposons amplify via linear, single-stranded cDNA intermediates.