id: A0A8B6GS20
gene_symbol: A0A8B6GS20
taxon:
  id: NCBITaxon:29158
  label: Mytilus galloprovincialis
status: COMPLETE
description: >-
  The deposited myotubularin-like domain lacks the catalytic CX5R motif, arguing against intrinsic phosphatidylinositol-3-phosphate
  phosphatase activity.
source_documents:
  - genes/MYTGA/A0A8B6GS20/A0A8B6GS20-uniprot.txt
  - genes/MYTGA/A0A8B6GS20/A0A8B6GS20-goa.tsv
  - publications/PMID_22647598.md
  - genes/MYTGA/A0A8B6GS20/A0A8B6GS20-hypotheses/prediction-pi3p-phosphatase/openscientist.md
predictions:
  - source_method: ProtNLM2
    source_version: UniProt 2024_06 pilot
    predicted_term:
      id: GO:0004438
      label: phosphatidylinositol-3-phosphate phosphatase activity
    predicted_term_type: GO_MF
    review:
      assessment: NPI
      confidence_score: 0
      summary: >-
        The target has a myotubularin-like phosphatase domain at residues 372–544, but this interval contains
        no CX5R catalytic motif. Experiments on MTMR complexes distinguish active phosphatases from inactive
        family members that lack the catalytic cysteine and regulate their partners (PMID:22647598). This
        sequence-level deficiency argues against the predicted intrinsic PI3P phosphatase activity even
        though the protein could influence phosphoinositide metabolism through a partner. The unusual
        N-terminal domain arrangement and exact MTMR paralog identity remain unresolved; the activity
        judgment does not depend on calling the target MTMR9.
      error_type: PSEUDOENZYME_OVERANNOTATION
      supported_by:
        - reference_id: file:MYTGA/A0A8B6GS20/A0A8B6GS20-uniprot.txt
          supporting_text: >-
            ID   A0A8B6GS20_MYTGA        Unreviewed;       607 AA. ... DR   InterPro; IPR010569; Myotubularin-like_Pase_dom.
            ... DR   PANTHER; PTHR10807:SF73; LD06050P; 1. ... DR   PANTHER; PTHR10807; MYOTUBULARIN-RELATED;
            1. ... FT   DOMAIN          372..544 ... FT                   /note="Myotubularin phosphatase"
        - reference_id: PMID:22647598
          supporting_text: >-
            inactive because they lack the conserved cysteine residue
        - reference_id: file:MYTGA/A0A8B6GS20/A0A8B6GS20-hypotheses/prediction-pi3p-phosphatase/openscientist.md
          supporting_text: >-
            This is the signature of a **catalytically dead myotubularin pseudophosphatase** and directly
            refutes the GO:0004438 prediction.
references:
  - id: file:MYTGA/A0A8B6GS20/A0A8B6GS20-uniprot.txt
    title: A0A8B6GS20-uniprot.txt
  - id: PMID:22647598
    title: Myotubularin-related protein (MTMR) 9 determines the enzymatic activity, substrate
      specificity, and role in autophagy of MTMR8.
  - id: file:MYTGA/A0A8B6GS20/A0A8B6GS20-hypotheses/prediction-pi3p-phosphatase/openscientist.md
    title: openscientist.md