NCU01245

UniProt ID: Q1K6K5
Organism: Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Review Status: COMPLETE
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Gene Description

NCU01245 is the conserved RPC4/C53 subunit of nuclear RNA polymerase III. It associates with the C37 subunit and supports promoter opening, transcription termination and polymerase recycling during synthesis of tRNAs and other polymerase III RNAs. Its role is structural and regulatory within the enzyme complex.

Existing Annotations Review

GO Term Evidence Action Reason
GO:0003677 DNA binding
IEA
GO_REF:0000002 		ACCEPT
Summary: NCU01245 contains the RPC4-specific domain and corresponds to the C53 subunit of RNA polymerase III.
Reason: NCU01245 contains the RPC4-specific domain and corresponds to the C53 subunit of RNA polymerase III. Photochemical cross-linking places characterized yeast C53 adjacent to the transcribed DNA strand; DNA contact occurs within the polymerase complex.
Supporting Evidence:
PMID:19940126
Protein-RNA and protein-DNA photochemical cross-linking analysis places a segment of C53 close to the RNA 3' end and transcribed DNA strand at the catalytic center of the pol III elongation complex.
GO:0005634 nucleus
IEA
GO_REF:0000044 		ACCEPT
Summary: The supported RNA polymerase III subunit assignment places NCU01245 in the nucleus, where fungal tRNA genes are transcribed.
Reason: The supported RNA polymerase III subunit assignment places NCU01245 in the nucleus, where fungal tRNA genes are transcribed. This localization follows from the conserved nuclear polymerase complex rather than the ARBA location assertion.
Supporting Evidence:
PMID:16362040
The specific requirement for the C37-C53 complex in terminator recognition was determined.
GO:0005666 RNA polymerase III complex
IBA
GO_REF:0000033 		ACCEPT
Summary: The RPC4-specific domain and subfamily identify the conserved C53/RPC4 subunit of RNA polymerase III.
Reason: The RPC4-specific domain and subfamily identify the conserved C53/RPC4 subunit of RNA polymerase III. Purified yeast polymerase reconstitution establishes its role with C37 in transcription termination and recycling.
Supporting Evidence:
PMID:16362040
The specific requirement for the C37-C53 complex in terminator recognition was determined.
GO:0005666 RNA polymerase III complex
IEA
GO_REF:0000002 		ACCEPT
Summary: The RPC4-specific domain and subfamily identify the conserved C53/RPC4 subunit of RNA polymerase III.
Reason: The RPC4-specific domain and subfamily identify the conserved C53/RPC4 subunit of RNA polymerase III. Purified yeast polymerase reconstitution establishes its role with C37 in transcription termination and recycling.
Supporting Evidence:
PMID:16362040
The specific requirement for the C37-C53 complex in terminator recognition was determined.
GO:0006383 transcription by RNA polymerase III
IEA
GO_REF:0000002 		ACCEPT
Summary: RPC4/C53 is a noncatalytic RNA polymerase III subunit that supports transcription of tRNA genes.
Reason: RPC4/C53 is a noncatalytic RNA polymerase III subunit that supports transcription of tRNA genes. Reconstituted yeast C37-C53 restores proper termination and, with C11, facilitates polymerase recycling; this justifies conserved process participation without assigning autonomous RNA polymerase activity.
Supporting Evidence:
PMID:16362040
The specific requirement for the C37-C53 complex in terminator recognition was determined.
PMID:19940126
Protein-RNA and protein-DNA photochemical cross-linking analysis places a segment of C53 close to the RNA 3' end and transcribed DNA strand at the catalytic center of the pol III elongation complex.
GO:0042797 tRNA transcription by RNA polymerase III
IBA
GO_REF:0000033 		ACCEPT
Summary: RPC4/C53 is a noncatalytic RNA polymerase III subunit that supports transcription of tRNA genes.
Reason: RPC4/C53 is a noncatalytic RNA polymerase III subunit that supports transcription of tRNA genes. Reconstituted yeast C37-C53 restores proper termination and, with C11, facilitates polymerase recycling; this justifies conserved process participation without assigning autonomous RNA polymerase activity.
Supporting Evidence:
PMID:16362040
The specific requirement for the C37-C53 complex in terminator recognition was determined.
PMID:19940126
Protein-RNA and protein-DNA photochemical cross-linking analysis places a segment of C53 close to the RNA 3' end and transcribed DNA strand at the catalytic center of the pol III elongation complex.

Core Functions

NCU01245 is the conserved RPC4/C53 subunit of nuclear RNA polymerase III. It associates with the C37 subunit and supports promoter opening, transcription termination and polymerase recycling during synthesis of tRNAs and other polymerase III RNAs. Its role is structural and regulatory within the enzyme complex.

Directly Involved In:
tRNA transcription by RNA polymerase III
Cellular Locations:
nucleus
In Complex:
RNA polymerase III complex
Supporting Evidence:
  • PMID:16362040
    The specific requirement for the C37-C53 complex in terminator recognition was determined.
  • PMID:19940126
    Protein-RNA and protein-DNA photochemical cross-linking analysis places a segment of C53 close to the RNA 3' end and transcribed DNA strand at the catalytic center of the pol III elongation complex.
  • file:NEUCR/NCU01245/NCU01245-uniprot.txt
    DR InterPro; IPR007811; RPC4.

References

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External Prediction Reviews

These computational predictions are reviewed separately from the GOA annotation set used for this review. The assessments below are from this project and do not constitute official GO annotations or endorsement by GO/UniProt. They are not included in the existing annotation review above.

ProtNLM2 External predictions

View prediction review YAML · NCU01245-protnlm-predictions-review.yaml · Review status: COMPLETE

NCU01245 is the conserved RPC4/C53 subunit of nuclear RNA polymerase III. It associates with the C37 subunit and supports promoter opening, transcription termination and polymerase recycling during synthesis of tRNAs and other polymerase III RNAs. Its role is structural and regulatory within the enzyme complex.

Source documents: genes/NEUCR/NCU01245/NCU01245-protnlm-source.json · genes/NEUCR/NCU01245/NCU01245-uniprot.txt · genes/NEUCR/NCU01245/NCU01245-ai-review.yaml

Review score: 2 = concordant with evidence; 1 = uncertain; 0 = discordant with evidence. This is an assessment score, not a model probability.

GO:0006383 transcription by RNA polymerase III GO_BP
LSP — Less precise than existing annotation Review score: 2/2
Prediction method: ProtNLM2 · Version: UniProt API snapshot 2026-09-09 · file:NEUCR/NCU01245/NCU01245-protnlm-source.json
Review rationale: The RPC4-specific domain identifies the C53 subunit of nuclear RNA polymerase III. Reconstitution of characterized yeast polymerase establishes transcriptional termination and recycling roles. GOA already includes tRNA transcription by RNA polymerase III (GO:0042797), so the emitted general polymerase III process is supported but less precise. This is conserved complex participation rather than autonomous polymerase catalysis.
Supporting Evidence:
  • PMID:16362040: "The specific requirement for the C37-C53 complex in terminator recognition was determined."
  • PMID:19940126: "Protein-RNA and protein-DNA photochemical cross-linking analysis places a segment of C53 close to the RNA 3' end and transcribed DNA strand at the catalytic center of the pol III elongation complex."
  • file:NEUCR/NCU01245/NCU01245-uniprot.txt: "DR InterPro; IPR007811; RPC4."

Deep Research

Falcon

(NCU01245-deep-research-falcon.md)

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📚 Additional Documentation

Notes

(NCU01245-notes.md)

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📄 View Raw YAML

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