id: Q1K6K5 gene_symbol: NCU01245 product_type: PROTEIN status: COMPLETE taxon: id: NCBITaxon:367110 label: Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) description: NCU01245 is the conserved RPC4/C53 subunit of nuclear RNA polymerase III. It associates with the C37 subunit and supports promoter opening, transcription termination and polymerase recycling during synthesis of tRNAs and other polymerase III RNAs. Its role is structural and regulatory within the enzyme complex. existing_annotations: - term: id: GO:0003677 label: DNA binding evidence_type: IEA original_reference_id: GO_REF:0000002 qualifier: enables supporting_entities: - InterPro:IPR007811 review: summary: NCU01245 contains the RPC4-specific domain and corresponds to the C53 subunit of RNA polymerase III. action: ACCEPT reason: NCU01245 contains the RPC4-specific domain and corresponds to the C53 subunit of RNA polymerase III. Photochemical cross-linking places characterized yeast C53 adjacent to the transcribed DNA strand; DNA contact occurs within the polymerase complex. supported_by: - reference_id: PMID:19940126 supporting_text: 'Protein-RNA and protein-DNA photochemical cross-linking analysis places a segment of C53 close to the RNA 3'' end and transcribed DNA strand at the catalytic center of the pol III elongation complex.' - term: &id003 id: GO:0005634 label: nucleus evidence_type: IEA original_reference_id: GO_REF:0000044 qualifier: located_in supporting_entities: - UniProtKB-SubCell:SL-0191 review: summary: The supported RNA polymerase III subunit assignment places NCU01245 in the nucleus, where fungal tRNA genes are transcribed. action: ACCEPT reason: The supported RNA polymerase III subunit assignment places NCU01245 in the nucleus, where fungal tRNA genes are transcribed. This localization follows from the conserved nuclear polymerase complex rather than the ARBA location assertion. supported_by: - reference_id: PMID:16362040 supporting_text: 'The specific requirement for the C37-C53 complex in terminator recognition was determined.' - term: id: GO:0005666 label: RNA polymerase III complex evidence_type: IBA original_reference_id: GO_REF:0000033 qualifier: part_of supporting_entities: - PANTHER:PTN000334172 - SGD:S000002309 - UniProtKB:P05423 review: summary: The RPC4-specific domain and subfamily identify the conserved C53/RPC4 subunit of RNA polymerase III. action: ACCEPT reason: The RPC4-specific domain and subfamily identify the conserved C53/RPC4 subunit of RNA polymerase III. Purified yeast polymerase reconstitution establishes its role with C37 in transcription termination and recycling. supported_by: - reference_id: PMID:16362040 supporting_text: 'The specific requirement for the C37-C53 complex in terminator recognition was determined.' - term: &id002 id: GO:0005666 label: RNA polymerase III complex evidence_type: IEA original_reference_id: GO_REF:0000002 qualifier: part_of supporting_entities: - InterPro:IPR007811 review: summary: The RPC4-specific domain and subfamily identify the conserved C53/RPC4 subunit of RNA polymerase III. action: ACCEPT reason: The RPC4-specific domain and subfamily identify the conserved C53/RPC4 subunit of RNA polymerase III. Purified yeast polymerase reconstitution establishes its role with C37 in transcription termination and recycling. supported_by: - reference_id: PMID:16362040 supporting_text: 'The specific requirement for the C37-C53 complex in terminator recognition was determined.' - term: id: GO:0006383 label: transcription by RNA polymerase III evidence_type: IEA original_reference_id: GO_REF:0000002 qualifier: involved_in supporting_entities: - InterPro:IPR007811 review: summary: RPC4/C53 is a noncatalytic RNA polymerase III subunit that supports transcription of tRNA genes. action: ACCEPT reason: RPC4/C53 is a noncatalytic RNA polymerase III subunit that supports transcription of tRNA genes. Reconstituted yeast C37-C53 restores proper termination and, with C11, facilitates polymerase recycling; this justifies conserved process participation without assigning autonomous RNA polymerase activity. supported_by: - reference_id: PMID:16362040 supporting_text: 'The specific requirement for the C37-C53 complex in terminator recognition was determined.' - reference_id: PMID:19940126 supporting_text: 'Protein-RNA and protein-DNA photochemical cross-linking analysis places a segment of C53 close to the RNA 3'' end and transcribed DNA strand at the catalytic center of the pol III elongation complex.' - term: &id001 id: GO:0042797 label: tRNA transcription by RNA polymerase III evidence_type: IBA original_reference_id: GO_REF:0000033 qualifier: involved_in supporting_entities: - PANTHER:PTN000334172 - SGD:S000002309 review: summary: RPC4/C53 is a noncatalytic RNA polymerase III subunit that supports transcription of tRNA genes. action: ACCEPT reason: RPC4/C53 is a noncatalytic RNA polymerase III subunit that supports transcription of tRNA genes. Reconstituted yeast C37-C53 restores proper termination and, with C11, facilitates polymerase recycling; this justifies conserved process participation without assigning autonomous RNA polymerase activity. supported_by: - reference_id: PMID:16362040 supporting_text: 'The specific requirement for the C37-C53 complex in terminator recognition was determined.' - reference_id: PMID:19940126 supporting_text: 'Protein-RNA and protein-DNA photochemical cross-linking analysis places a segment of C53 close to the RNA 3'' end and transcribed DNA strand at the catalytic center of the pol III elongation complex.' references: - id: GO_REF:0000002 title: Gene Ontology annotation through association of InterPro records with GO terms findings: [] - id: GO_REF:0000033 title: Annotation inferences using phylogenetic trees findings: [] - id: GO_REF:0000044 title: Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt findings: [] - id: PMID:19940126 title: The C53/C37 subcomplex of RNA polymerase III lies near the active site and participates in promoter opening. full_text_unavailable: true - id: PMID:16362040 title: A subcomplex of RNA polymerase III subunits involved in transcription termination and reinitiation. full_text_unavailable: false - id: file:NEUCR/NCU01245/NCU01245-uniprot.txt title: 'UniProt record for NCU01245: domain and family evidence' core_functions: - description: NCU01245 is the conserved RPC4/C53 subunit of nuclear RNA polymerase III. It associates with the C37 subunit and supports promoter opening, transcription termination and polymerase recycling during synthesis of tRNAs and other polymerase III RNAs. Its role is structural and regulatory within the enzyme complex. supported_by: - reference_id: PMID:16362040 supporting_text: 'The specific requirement for the C37-C53 complex in terminator recognition was determined.' - reference_id: PMID:19940126 supporting_text: 'Protein-RNA and protein-DNA photochemical cross-linking analysis places a segment of C53 close to the RNA 3'' end and transcribed DNA strand at the catalytic center of the pol III elongation complex.' - reference_id: file:NEUCR/NCU01245/NCU01245-uniprot.txt supporting_text: DR InterPro; IPR007811; RPC4. directly_involved_in: - *id001 in_complex: *id002 locations: - *id003