id: Q7SHS5
gene_symbol: NCU02539
taxon:
  id: NCBITaxon:367110
  label: Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
status: COMPLETE
description: The emitted broad GO predictions are supported but less precise than existing defensible
  annotations.
source_documents:
- genes/NEUCR/NCU02539/NCU02539-protnlm-source.json
- genes/NEUCR/NCU02539/NCU02539-uniprot.txt
predictions:
- source_method: ProtNLM2
  source_version: UniProt API snapshot 2026-09-09
  source_reference_id: file:NEUCR/NCU02539/NCU02539-protnlm-source.json
  predicted_term:
    id: GO:0033260
    label: nuclear DNA replication
  predicted_term_type: GO_BP
  review:
    assessment: LSP
    confidence_score: 2
    summary: InterPro MCM_4 and the Mcm4-specific PANTHER assignment identify a conserved replicative
      helicase subunit. Reconstitution of Mcm2-7 origin loading and biochemical study of Mcm4-containing
      complexes support participation in nuclear DNA replication. GOA already includes the more specific
      mitotic DNA replication initiation and DNA strand elongation functions. The broad prediction is
      therefore less precise; its hydration from premeiotic replication is provenance rather than independent
      evidence for a Neurospora meiotic assay.
    supported_by:
    - reference_id: file:NEUCR/NCU02539/NCU02539-uniprot.txt
      supporting_text: DR   InterPro; IPR008047; MCM_4.
    - reference_id: PMID:19896182
      supporting_text: The licensing of eukaryotic DNA replication origins, which ensures once-per-cell-cycle
        replication, involves the loading of six related minichromosome maintenance proteins (Mcm2-7)
        into prereplicative complexes (pre-RCs).
    - reference_id: PMID:10567526
      supporting_text: the Mcm4 protein may play a role in the single-stranded DNA binding activity of
        the complex.
    - reference_id: file:NEUCR/NCU02539/NCU02539-uniprot.txt
      supporting_text: DR   GO; GO:0003697; F:single-stranded DNA binding; IBA:GO_Central.
- source_method: ProtNLM2
  source_version: UniProt API snapshot 2026-09-09
  source_reference_id: file:NEUCR/NCU02539/NCU02539-protnlm-source.json
  predicted_term:
    id: GO:0006270
    label: DNA replication initiation
  predicted_term_type: GO_BP
  review:
    assessment: LSP
    confidence_score: 2
    summary: The Mcm4-specific sequence assignment supports membership in the Mcm2-7 helicase. Purified
      budding-yeast Mcm2-7 loading establishes the conserved role in origin licensing, a prerequisite
      for initiation. The existing curated phylogenetic annotation specifies mitotic DNA replication initiation,
      making the generic initiation prediction less precise. The judgment concerns participation of the
      Mcm4 subunit in the complex, not autonomous initiation activity.
    supported_by:
    - reference_id: file:NEUCR/NCU02539/NCU02539-uniprot.txt
      supporting_text: DR   InterPro; IPR008047; MCM_4.
    - reference_id: PMID:19896182
      supporting_text: The licensing of eukaryotic DNA replication origins, which ensures once-per-cell-cycle
        replication, involves the loading of six related minichromosome maintenance proteins (Mcm2-7)
        into prereplicative complexes (pre-RCs).
    - reference_id: PMID:10567526
      supporting_text: the Mcm4 protein may play a role in the single-stranded DNA binding activity of
        the complex.
    - reference_id: file:NEUCR/NCU02539/NCU02539-uniprot.txt
      supporting_text: DR   GO; GO:0003697; F:single-stranded DNA binding; IBA:GO_Central.
- source_method: ProtNLM2
  source_version: UniProt API snapshot 2026-09-09
  source_reference_id: file:NEUCR/NCU02539/NCU02539-protnlm-source.json
  predicted_term:
    id: GO:0003677
    label: DNA binding
  predicted_term_type: GO_MF
  review:
    assessment: LSP
    confidence_score: 2
    summary: The target has the Mcm4-specific MCM domain architecture, including an OB-fold DNA-associated
      region. Biochemical analysis of Mcm4-containing complexes supports a contribution to single-stranded
      DNA binding, which is already annotated through PAINT. The generic DNA-binding prediction loses
      that substrate specificity. Binding and helicase activity are properties of the assembled MCM machinery,
      not evidence for an independent monomeric DNA-binding regulator.
    supported_by:
    - reference_id: file:NEUCR/NCU02539/NCU02539-uniprot.txt
      supporting_text: DR   InterPro; IPR008047; MCM_4.
    - reference_id: PMID:19896182
      supporting_text: The licensing of eukaryotic DNA replication origins, which ensures once-per-cell-cycle
        replication, involves the loading of six related minichromosome maintenance proteins (Mcm2-7)
        into prereplicative complexes (pre-RCs).
    - reference_id: PMID:10567526
      supporting_text: the Mcm4 protein may play a role in the single-stranded DNA binding activity of
        the complex.
    - reference_id: file:NEUCR/NCU02539/NCU02539-uniprot.txt
      supporting_text: DR   GO; GO:0003697; F:single-stranded DNA binding; IBA:GO_Central.
references:
- id: file:NEUCR/NCU02539/NCU02539-uniprot.txt
  title: NCU02539-uniprot.txt
  findings: []
- id: PMID:19896182
  title: Concerted loading of Mcm2-7 double hexamers around DNA during DNA replication origin licensing.
  findings: []
  full_text_unavailable: false
- id: PMID:10567526
  title: Biochemical analysis of the intrinsic Mcm4-Mcm6-mcm7 DNA helicase activity.
  findings: []
  full_text_unavailable: true