NCU03033

UniProt ID: Q7SGY4
Organism: Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Review Status: COMPLETE
📝 Provide Detailed Feedback

Gene Description

NCU03033 is the Hap2/NF-YA subunit of the heterotrimeric CCAAT-binding transcription factor. Its conserved DNA-recognition domain works with the two partner subunits to bind CCAAT promoter elements and regulate nuclear RNA polymerase II transcription.

Existing Annotations Review

GO Term Evidence Action Reason
GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific
IBA
GO_REF:0000033 		ACCEPT
Summary: The NF-YA/Hap2-specific domain identifies the sequence-recognition subunit of the conserved CCAAT-binding transcription factor.
Reason: The NF-YA/Hap2-specific domain identifies the sequence-recognition subunit of the conserved CCAAT-binding transcription factor. Reconstituted Aspergillus HapB-HapC-HapE binds CCAAT DNA and regulates RNA polymerase II-transcribed genes, supporting transfer of this complex and transcription-factor function.
Supporting Evidence:
PMID:25589790
The CBC consists of three subunits, which are named in the filamentous fungus Aspergillus nidulans HapB, HapC, and HapE.
PMID:25589790
Initial binding of the CBC to CCAAT boxes is mandatory for DNA recognition of HapX.
PMID:19965775
The corresponding Aspergillus nidulans CCAAT-binding factor (AnCF) consists of the subunits HapB, HapC and HapE. All of the three subunits are necessary for DNA binding.
GO:0003677 DNA binding
IEA
GO_REF:0000104 		MODIFY
Summary: DNA binding and generic transcription-factor activity understate the conserved function: the Hap2/NF-YA subunit participates in CCAAT recognition within a RNA polymerase II transcription factor complex..
Reason: DNA binding and generic transcription-factor activity understate the conserved function: the Hap2/NF-YA subunit participates in CCAAT recognition within a RNA polymerase II transcription factor complex.
Supporting Evidence:
PMID:25589790
The CBC consists of three subunits, which are named in the filamentous fungus Aspergillus nidulans HapB, HapC, and HapE.
PMID:25589790
Initial binding of the CBC to CCAAT boxes is mandatory for DNA recognition of HapX.
GO:0003700 DNA-binding transcription factor activity
IEA
GO_REF:0000120 		MODIFY
Summary: DNA binding and generic transcription-factor activity understate the conserved function: the Hap2/NF-YA subunit participates in CCAAT recognition within a RNA polymerase II transcription factor complex..
Reason: DNA binding and generic transcription-factor activity understate the conserved function: the Hap2/NF-YA subunit participates in CCAAT recognition within a RNA polymerase II transcription factor complex.
Supporting Evidence:
PMID:25589790
The CBC consists of three subunits, which are named in the filamentous fungus Aspergillus nidulans HapB, HapC, and HapE.
PMID:25589790
Initial binding of the CBC to CCAAT boxes is mandatory for DNA recognition of HapX.
GO:0005634 nucleus
IEA
GO_REF:0000120 		ACCEPT
Summary: The conserved NF-YA/Hap2 CCAAT-binding transcription factor acts on nuclear gene promoters.
Reason: The conserved NF-YA/Hap2 CCAAT-binding transcription factor acts on nuclear gene promoters. This localization is justified by specific family membership and characterized fungal DNA-binding complexes, rather than automatic nucleus keyword agreement.
Supporting Evidence:
PMID:25589790
The CBC consists of three subunits, which are named in the filamentous fungus Aspergillus nidulans HapB, HapC, and HapE.
PMID:25589790
Initial binding of the CBC to CCAAT boxes is mandatory for DNA recognition of HapX.
GO:0006351 DNA-templated transcription
IEA
GO_REF:0000104 		MODIFY
Summary: Hap2 is a sequence-specific transcriptional regulator rather than an RNA-synthesizing enzyme.
Reason: Hap2 is a sequence-specific transcriptional regulator rather than an RNA-synthesizing enzyme. Regulation of transcription by RNA polymerase II is the supported, more informative process.
Supporting Evidence:
PMID:25589790
The CBC consists of three subunits, which are named in the filamentous fungus Aspergillus nidulans HapB, HapC, and HapE.
PMID:25589790
Initial binding of the CBC to CCAAT boxes is mandatory for DNA recognition of HapX.
GO:0006355 regulation of DNA-templated transcription
IEA
GO_REF:0000120 		MODIFY
Summary: Hap2 is a sequence-specific transcriptional regulator rather than an RNA-synthesizing enzyme.
Reason: Hap2 is a sequence-specific transcriptional regulator rather than an RNA-synthesizing enzyme. Regulation of transcription by RNA polymerase II is the supported, more informative process.
Supporting Evidence:
PMID:25589790
The CBC consists of three subunits, which are named in the filamentous fungus Aspergillus nidulans HapB, HapC, and HapE.
PMID:25589790
Initial binding of the CBC to CCAAT boxes is mandatory for DNA recognition of HapX.
GO:0006357 regulation of transcription by RNA polymerase II
IBA
GO_REF:0000033 		ACCEPT
Summary: The conserved CCAAT-binding factor regulates RNA polymerase II promoters.
Reason: The conserved CCAAT-binding factor regulates RNA polymerase II promoters. Aspergillus DNA-binding and transcriptional-regulation experiments support this process for the NF-YA/Hap2 subfamily; specific target genes or iron phenotypes are not transferred to Neurospora.
Supporting Evidence:
PMID:25589790
The CBC consists of three subunits, which are named in the filamentous fungus Aspergillus nidulans HapB, HapC, and HapE.
PMID:25589790
Initial binding of the CBC to CCAAT boxes is mandatory for DNA recognition of HapX.
GO:0016602 CCAAT-binding factor complex
IBA
GO_REF:0000033 		ACCEPT
Summary: The NF-YA/Hap2-specific domain identifies the sequence-recognition subunit of the conserved CCAAT-binding transcription factor.
Reason: The NF-YA/Hap2-specific domain identifies the sequence-recognition subunit of the conserved CCAAT-binding transcription factor. Reconstituted Aspergillus HapB-HapC-HapE binds CCAAT DNA and regulates RNA polymerase II-transcribed genes, supporting transfer of this complex and transcription-factor function.
Supporting Evidence:
PMID:25589790
The CBC consists of three subunits, which are named in the filamentous fungus Aspergillus nidulans HapB, HapC, and HapE.
PMID:25589790
Initial binding of the CBC to CCAAT boxes is mandatory for DNA recognition of HapX.
PMID:19965775
The corresponding Aspergillus nidulans CCAAT-binding factor (AnCF) consists of the subunits HapB, HapC and HapE. All of the three subunits are necessary for DNA binding.
GO:0016602 CCAAT-binding factor complex
IEA
GO_REF:0000104 		ACCEPT
Summary: The NF-YA/Hap2-specific domain identifies the sequence-recognition subunit of the conserved CCAAT-binding transcription factor.
Reason: The NF-YA/Hap2-specific domain identifies the sequence-recognition subunit of the conserved CCAAT-binding transcription factor. Reconstituted Aspergillus HapB-HapC-HapE binds CCAAT DNA and regulates RNA polymerase II-transcribed genes, supporting transfer of this complex and transcription-factor function.
Supporting Evidence:
PMID:25589790
The CBC consists of three subunits, which are named in the filamentous fungus Aspergillus nidulans HapB, HapC, and HapE.
PMID:25589790
Initial binding of the CBC to CCAAT boxes is mandatory for DNA recognition of HapX.
PMID:19965775
The corresponding Aspergillus nidulans CCAAT-binding factor (AnCF) consists of the subunits HapB, HapC and HapE. All of the three subunits are necessary for DNA binding.

Core Functions

NCU03033 is the Hap2/NF-YA subunit of the heterotrimeric CCAAT-binding transcription factor. Its conserved DNA-recognition domain works with the two partner subunits to bind CCAAT promoter elements and regulate nuclear RNA polymerase II transcription.

Molecular Function:
DNA-binding transcription factor activity, RNA polymerase II-specific
Directly Involved In:
regulation of transcription by RNA polymerase II
Cellular Locations:
nucleus
In Complex:
CCAAT-binding factor complex
Supporting Evidence:
  • PMID:25589790
    The CBC consists of three subunits, which are named in the filamentous fungus Aspergillus nidulans HapB, HapC, and HapE.
  • PMID:25589790
    Initial binding of the CBC to CCAAT boxes is mandatory for DNA recognition of HapX.
  • file:NEUCR/NCU03033/NCU03033-uniprot.txt
    DR InterPro; IPR001289; NFYA.
  • PMID:19965775
    The corresponding Aspergillus nidulans CCAAT-binding factor (AnCF) consists of the subunits HapB, HapC and HapE. All of the three subunits are necessary for DNA binding.

References

Loading supporting content…

Download this section (compressed HTML)

External Prediction Reviews

These computational predictions are reviewed separately from the GOA annotation set used for this review. The assessments below are from this project and do not constitute official GO annotations or endorsement by GO/UniProt. They are not included in the existing annotation review above.

ProtNLM2 External predictions

View prediction review YAML · NCU03033-protnlm-predictions-review.yaml · Review status: COMPLETE

NCU03033 is the Hap2/NF-YA subunit of the heterotrimeric CCAAT-binding transcription factor. Its conserved DNA-recognition domain works with the two partner subunits to bind CCAAT promoter elements and regulate nuclear RNA polymerase II transcription.

Source documents: genes/NEUCR/NCU03033/NCU03033-protnlm-source.json · genes/NEUCR/NCU03033/NCU03033-uniprot.txt · genes/NEUCR/NCU03033/NCU03033-ai-review.yaml

Review score: 2 = concordant with evidence; 1 = uncertain; 0 = discordant with evidence. This is an assessment score, not a model probability.

GO:0003700 DNA-binding transcription factor activity GO_MF
LSP — Less precise than existing annotation Review score: 2/2
Prediction method: ProtNLM2 · Version: UniProt API snapshot 2026-09-09 · file:NEUCR/NCU03033/NCU03033-protnlm-source.json
Review rationale: The NF-YA/Hap2 domain and characterized fungal CCAAT-binding factor establish sequence-specific promoter recognition and transcription regulation. GOA already supplies RNA polymerase II-specific DNA-binding transcription factor activity (GO:0000981), so the generic activity prediction is less precise.
Supporting Evidence:
  • PMID:25589790: "The CBC consists of three subunits, which are named in the filamentous fungus Aspergillus nidulans HapB, HapC, and HapE."
  • PMID:25589790: "Initial binding of the CBC to CCAAT boxes is mandatory for DNA recognition of HapX."
  • file:NEUCR/NCU03033/NCU03033-uniprot.txt: "DR InterPro; IPR001289; NFYA."
  • PMID:19965775: "The corresponding Aspergillus nidulans CCAAT-binding factor (AnCF) consists of the subunits HapB, HapC and HapE. All of the three subunits are necessary for DNA binding."
GO:0005634 nucleus GO_CC
CNN — Correct but not novel Review score: 2/2
Prediction method: ProtNLM2 · Version: UniProt API snapshot 2026-09-09 · file:NEUCR/NCU03033/NCU03033-protnlm-source.json
Review rationale: The NF-YA/Hap2 family identifies a conserved CCAAT-binding transcription-factor subunit acting on nuclear promoters. Fungal biochemical experiments establish the DNA-bound complex; its nuclear function follows from that mechanism. An equivalent nucleus annotation is already in GOA and the family-based location statement.
Supporting Evidence:
  • PMID:25589790: "The CBC consists of three subunits, which are named in the filamentous fungus Aspergillus nidulans HapB, HapC, and HapE."
  • PMID:25589790: "Initial binding of the CBC to CCAAT boxes is mandatory for DNA recognition of HapX."
  • file:NEUCR/NCU03033/NCU03033-uniprot.txt: "DR InterPro; IPR001289; NFYA."
  • PMID:19965775: "The corresponding Aspergillus nidulans CCAAT-binding factor (AnCF) consists of the subunits HapB, HapC and HapE. All of the three subunits are necessary for DNA binding."

Deep Research

Falcon

(NCU03033-deep-research-falcon.md)

Loading supporting content…

Download this section (compressed HTML)

📚 Additional Documentation

Notes

(NCU03033-notes.md)

Loading supporting content…

Download this section (compressed HTML)

📄 View Raw YAML

Loading supporting content…

Download this section (compressed HTML)