id: Q7SGY4 gene_symbol: NCU03033 product_type: PROTEIN status: COMPLETE taxon: id: NCBITaxon:367110 label: Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) description: NCU03033 is the Hap2/NF-YA subunit of the heterotrimeric CCAAT-binding transcription factor. Its conserved DNA-recognition domain works with the two partner subunits to bind CCAAT promoter elements and regulate nuclear RNA polymerase II transcription. existing_annotations: - term: &id003 id: GO:0000981 label: DNA-binding transcription factor activity, RNA polymerase II-specific evidence_type: IBA original_reference_id: GO_REF:0000033 qualifier: enables supporting_entities: - MGI:MGI:97316 - PANTHER:PTN000290575 - SGD:S000003206 - UniProtKB:P23511 review: summary: The NF-YA/Hap2-specific domain identifies the sequence-recognition subunit of the conserved CCAAT-binding transcription factor. action: ACCEPT reason: The NF-YA/Hap2-specific domain identifies the sequence-recognition subunit of the conserved CCAAT-binding transcription factor. Reconstituted Aspergillus HapB-HapC-HapE binds CCAAT DNA and regulates RNA polymerase II-transcribed genes, supporting transfer of this complex and transcription-factor function. supported_by: - reference_id: PMID:25589790 supporting_text: 'The CBC consists of three subunits, which are named in the filamentous fungus Aspergillus nidulans HapB, HapC, and HapE.' - reference_id: PMID:25589790 supporting_text: 'Initial binding of the CBC to CCAAT boxes is mandatory for DNA recognition of HapX.' - &id001 reference_id: PMID:19965775 supporting_text: 'The corresponding Aspergillus nidulans CCAAT-binding factor (AnCF) consists of the subunits HapB, HapC and HapE. All of the three subunits are necessary for DNA binding.' - term: id: GO:0003677 label: DNA binding evidence_type: IEA original_reference_id: GO_REF:0000104 qualifier: enables supporting_entities: - UniRule:UR000401053 review: summary: 'DNA binding and generic transcription-factor activity understate the conserved function: the Hap2/NF-YA subunit participates in CCAAT recognition within a RNA polymerase II transcription factor complex..' action: MODIFY reason: 'DNA binding and generic transcription-factor activity understate the conserved function: the Hap2/NF-YA subunit participates in CCAAT recognition within a RNA polymerase II transcription factor complex.' supported_by: - reference_id: PMID:25589790 supporting_text: 'The CBC consists of three subunits, which are named in the filamentous fungus Aspergillus nidulans HapB, HapC, and HapE.' - reference_id: PMID:25589790 supporting_text: 'Initial binding of the CBC to CCAAT boxes is mandatory for DNA recognition of HapX.' proposed_replacement_terms: - id: GO:0000981 label: DNA-binding transcription factor activity, RNA polymerase II-specific - term: id: GO:0003700 label: DNA-binding transcription factor activity evidence_type: IEA original_reference_id: GO_REF:0000120 qualifier: enables supporting_entities: - InterPro:IPR001289 - UniRule:UR000401053 - UniRule:UR001453790 review: summary: 'DNA binding and generic transcription-factor activity understate the conserved function: the Hap2/NF-YA subunit participates in CCAAT recognition within a RNA polymerase II transcription factor complex..' action: MODIFY reason: 'DNA binding and generic transcription-factor activity understate the conserved function: the Hap2/NF-YA subunit participates in CCAAT recognition within a RNA polymerase II transcription factor complex.' supported_by: - reference_id: PMID:25589790 supporting_text: 'The CBC consists of three subunits, which are named in the filamentous fungus Aspergillus nidulans HapB, HapC, and HapE.' - reference_id: PMID:25589790 supporting_text: 'Initial binding of the CBC to CCAAT boxes is mandatory for DNA recognition of HapX.' proposed_replacement_terms: - id: GO:0000981 label: DNA-binding transcription factor activity, RNA polymerase II-specific - term: &id005 id: GO:0005634 label: nucleus evidence_type: IEA original_reference_id: GO_REF:0000120 qualifier: located_in supporting_entities: - UniProtKB-SubCell:SL-0191 - UniRule:UR000401053 - UniRule:UR001453790 review: summary: The conserved NF-YA/Hap2 CCAAT-binding transcription factor acts on nuclear gene promoters. action: ACCEPT reason: The conserved NF-YA/Hap2 CCAAT-binding transcription factor acts on nuclear gene promoters. This localization is justified by specific family membership and characterized fungal DNA-binding complexes, rather than automatic nucleus keyword agreement. supported_by: - reference_id: PMID:25589790 supporting_text: 'The CBC consists of three subunits, which are named in the filamentous fungus Aspergillus nidulans HapB, HapC, and HapE.' - reference_id: PMID:25589790 supporting_text: 'Initial binding of the CBC to CCAAT boxes is mandatory for DNA recognition of HapX.' - term: id: GO:0006351 label: DNA-templated transcription evidence_type: IEA original_reference_id: GO_REF:0000104 qualifier: involved_in supporting_entities: - UniRule:UR000401053 review: summary: Hap2 is a sequence-specific transcriptional regulator rather than an RNA-synthesizing enzyme. action: MODIFY reason: Hap2 is a sequence-specific transcriptional regulator rather than an RNA-synthesizing enzyme. Regulation of transcription by RNA polymerase II is the supported, more informative process. supported_by: - reference_id: PMID:25589790 supporting_text: 'The CBC consists of three subunits, which are named in the filamentous fungus Aspergillus nidulans HapB, HapC, and HapE.' - reference_id: PMID:25589790 supporting_text: 'Initial binding of the CBC to CCAAT boxes is mandatory for DNA recognition of HapX.' proposed_replacement_terms: - id: GO:0006357 label: regulation of transcription by RNA polymerase II - term: id: GO:0006355 label: regulation of DNA-templated transcription evidence_type: IEA original_reference_id: GO_REF:0000120 qualifier: involved_in supporting_entities: - InterPro:IPR001289 - UniRule:UR001453790 review: summary: Hap2 is a sequence-specific transcriptional regulator rather than an RNA-synthesizing enzyme. action: MODIFY reason: Hap2 is a sequence-specific transcriptional regulator rather than an RNA-synthesizing enzyme. Regulation of transcription by RNA polymerase II is the supported, more informative process. supported_by: - reference_id: PMID:25589790 supporting_text: 'The CBC consists of three subunits, which are named in the filamentous fungus Aspergillus nidulans HapB, HapC, and HapE.' - reference_id: PMID:25589790 supporting_text: 'Initial binding of the CBC to CCAAT boxes is mandatory for DNA recognition of HapX.' proposed_replacement_terms: - id: GO:0006357 label: regulation of transcription by RNA polymerase II - term: &id002 id: GO:0006357 label: regulation of transcription by RNA polymerase II evidence_type: IBA original_reference_id: GO_REF:0000033 qualifier: involved_in supporting_entities: - MGI:MGI:97316 - PANTHER:PTN000290575 - SGD:S000003206 - UniProtKB:P23511 - WB:WBGene00011614 review: summary: The conserved CCAAT-binding factor regulates RNA polymerase II promoters. action: ACCEPT reason: The conserved CCAAT-binding factor regulates RNA polymerase II promoters. Aspergillus DNA-binding and transcriptional-regulation experiments support this process for the NF-YA/Hap2 subfamily; specific target genes or iron phenotypes are not transferred to Neurospora. supported_by: - reference_id: PMID:25589790 supporting_text: 'The CBC consists of three subunits, which are named in the filamentous fungus Aspergillus nidulans HapB, HapC, and HapE.' - reference_id: PMID:25589790 supporting_text: 'Initial binding of the CBC to CCAAT boxes is mandatory for DNA recognition of HapX.' - term: id: GO:0016602 label: CCAAT-binding factor complex evidence_type: IBA original_reference_id: GO_REF:0000033 qualifier: part_of supporting_entities: - FB:FBgn0035993 - PANTHER:PTN000290576 - RGD:70976 - SGD:S000003206 - UniProtKB:P23511 - WB:WBGene00011614 - WB:WBGene00043056 review: summary: The NF-YA/Hap2-specific domain identifies the sequence-recognition subunit of the conserved CCAAT-binding transcription factor. action: ACCEPT reason: The NF-YA/Hap2-specific domain identifies the sequence-recognition subunit of the conserved CCAAT-binding transcription factor. Reconstituted Aspergillus HapB-HapC-HapE binds CCAAT DNA and regulates RNA polymerase II-transcribed genes, supporting transfer of this complex and transcription-factor function. supported_by: - reference_id: PMID:25589790 supporting_text: 'The CBC consists of three subunits, which are named in the filamentous fungus Aspergillus nidulans HapB, HapC, and HapE.' - reference_id: PMID:25589790 supporting_text: 'Initial binding of the CBC to CCAAT boxes is mandatory for DNA recognition of HapX.' - *id001 - term: &id004 id: GO:0016602 label: CCAAT-binding factor complex evidence_type: IEA original_reference_id: GO_REF:0000104 qualifier: part_of supporting_entities: - UniRule:UR000401053 review: summary: The NF-YA/Hap2-specific domain identifies the sequence-recognition subunit of the conserved CCAAT-binding transcription factor. action: ACCEPT reason: The NF-YA/Hap2-specific domain identifies the sequence-recognition subunit of the conserved CCAAT-binding transcription factor. Reconstituted Aspergillus HapB-HapC-HapE binds CCAAT DNA and regulates RNA polymerase II-transcribed genes, supporting transfer of this complex and transcription-factor function. supported_by: - reference_id: PMID:25589790 supporting_text: 'The CBC consists of three subunits, which are named in the filamentous fungus Aspergillus nidulans HapB, HapC, and HapE.' - reference_id: PMID:25589790 supporting_text: 'Initial binding of the CBC to CCAAT boxes is mandatory for DNA recognition of HapX.' - *id001 references: - id: GO_REF:0000033 title: Annotation inferences using phylogenetic trees findings: [] - id: GO_REF:0000104 title: Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features findings: [] - id: GO_REF:0000120 title: Combined Automated Annotation using Multiple IEA Methods findings: [] - id: PMID:25589790 title: Deciphering the combinatorial DNA-binding code of the CCAAT-binding complex and the iron-regulatory basic region leucine zipper (bZIP) transcription factor HapX. full_text_unavailable: true - id: file:NEUCR/NCU03033/NCU03033-uniprot.txt title: 'UniProt record for NCU03033: domain and family evidence' - id: PMID:19965775 title: The CCAAT-binding complex coordinates the oxidative stress response in eukaryotes. core_functions: - description: NCU03033 is the Hap2/NF-YA subunit of the heterotrimeric CCAAT-binding transcription factor. Its conserved DNA-recognition domain works with the two partner subunits to bind CCAAT promoter elements and regulate nuclear RNA polymerase II transcription. supported_by: - reference_id: PMID:25589790 supporting_text: 'The CBC consists of three subunits, which are named in the filamentous fungus Aspergillus nidulans HapB, HapC, and HapE.' - reference_id: PMID:25589790 supporting_text: 'Initial binding of the CBC to CCAAT boxes is mandatory for DNA recognition of HapX.' - reference_id: file:NEUCR/NCU03033/NCU03033-uniprot.txt supporting_text: DR InterPro; IPR001289; NFYA. - *id001 directly_involved_in: - *id002 molecular_function: *id003 in_complex: *id004 locations: - *id005