id: Q7SGY4
gene_symbol: NCU03033
locus_tag: NCU03033
taxon:
  id: NCBITaxon:367110
  label: Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
status: COMPLETE
description: NCU03033 is the Hap2/NF-YA subunit of the heterotrimeric CCAAT-binding transcription factor.
  Its conserved DNA-recognition domain works with the two partner subunits to bind CCAAT promoter elements
  and regulate nuclear RNA polymerase II transcription.
source_documents:
- genes/NEUCR/NCU03033/NCU03033-protnlm-source.json
- genes/NEUCR/NCU03033/NCU03033-uniprot.txt
- genes/NEUCR/NCU03033/NCU03033-ai-review.yaml
predictions:
- source_method: ProtNLM2
  source_version: UniProt API snapshot 2026-09-09
  source_reference_id: file:NEUCR/NCU03033/NCU03033-protnlm-source.json
  predicted_term:
    id: GO:0003700
    label: DNA-binding transcription factor activity
  predicted_term_type: GO_MF
  review:
    assessment: LSP
    confidence_score: 2
    summary: The NF-YA/Hap2 domain and characterized fungal CCAAT-binding factor establish sequence-specific
      promoter recognition and transcription regulation. GOA already supplies RNA polymerase II-specific
      DNA-binding transcription factor activity (GO:0000981), so the generic activity prediction is less
      precise.
    supported_by: &id001
    - reference_id: PMID:25589790
      supporting_text: 'The CBC consists of

        three subunits, which are named in the filamentous fungus Aspergillus nidulans

        HapB, HapC, and HapE.'
    - reference_id: PMID:25589790
      supporting_text: 'Initial

        binding of the CBC to CCAAT boxes is mandatory for DNA recognition of HapX.'
    - reference_id: file:NEUCR/NCU03033/NCU03033-uniprot.txt
      supporting_text: DR   InterPro; IPR001289; NFYA.
    - reference_id: PMID:19965775
      supporting_text: 'The corresponding Aspergillus nidulans CCAAT-binding factor (AnCF)

        consists of the subunits HapB, HapC and HapE. All of the three subunits are

        necessary for DNA binding.'
- source_method: ProtNLM2
  source_version: UniProt API snapshot 2026-09-09
  source_reference_id: file:NEUCR/NCU03033/NCU03033-protnlm-source.json
  predicted_term:
    id: GO:0005634
    label: nucleus
  predicted_term_type: GO_CC
  review:
    assessment: CNN
    confidence_score: 2
    summary: The NF-YA/Hap2 family identifies a conserved CCAAT-binding transcription-factor subunit acting
      on nuclear promoters. Fungal biochemical experiments establish the DNA-bound complex; its nuclear
      function follows from that mechanism. An equivalent nucleus annotation is already in GOA and the
      family-based location statement.
    supported_by: *id001
references:
- &id002
  id: PMID:25589790
  title: Deciphering the combinatorial DNA-binding code of the CCAAT-binding complex and the iron-regulatory
    basic region leucine zipper (bZIP) transcription factor HapX.
  full_text_unavailable: true
- *id002
- id: file:NEUCR/NCU03033/NCU03033-uniprot.txt
  title: 'UniProt record for NCU03033: domain and family evidence'
- id: file:NEUCR/NCU03033/NCU03033-protnlm-source.json
  title: Frozen live ProtNLM2 API response for NCU03033
- id: PMID:19965775
  title: The CCAAT-binding complex coordinates the oxidative stress response in eukaryotes.