id: Q1K772
gene_symbol: NCU04302
taxon:
  id: NCBITaxon:367110
  label: Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
status: COMPLETE
description: The emitted broad GO predictions are supported but less precise than existing defensible
  annotations.
source_documents:
- genes/NEUCR/NCU04302/NCU04302-protnlm-source.json
- genes/NEUCR/NCU04302/NCU04302-uniprot.txt
- genes/NEUCR/NCU04302/NCU04302-bioinformatics/RESULTS.md
predictions:
- source_method: ProtNLM2
  source_version: UniProt API snapshot 2026-09-09
  source_reference_id: file:NEUCR/NCU04302/NCU04302-protnlm-source.json
  predicted_term:
    id: GO:0016740
    label: transferase activity
  predicted_term_type: GO_MF
  review:
    assessment: LSP
    confidence_score: 2
    summary: The target is a Ubc9-family SUMO E2, supported by modifier-specific CDD UBCc_UBE2I and FunFam
      Ubc9 assignments, full-length similarity to characterized Ubc9, and the curated SUMO-specific PAINT
      inference. Characterized Ubc9 homologs form thioester intermediates with SUMO. Transferase activity
      is therefore correct but less precise than the existing SUMO conjugating enzyme activity. This broad
      GO result does not validate the separate ubiquitin-specific paragraph.
    supported_by:
    - reference_id: file:NEUCR/NCU04302/NCU04302-uniprot.txt
      supporting_text: DR   InterPro; IPR000608; UBC.
    - reference_id: PMID:9435231
      supporting_text: Moreover, recombinant yeast and mammalian UBC9 enzymes were found to form thioester
        complexes with SMT3 and SUMO-1, respectively.
    - reference_id: file:NEUCR/NCU04302/NCU04302-uniprot.txt
      supporting_text: DR   CDD; cd23798; UBCc_UBE2I; 1.
    - reference_id: file:NEUCR/NCU04302/NCU04302-uniprot.txt
      supporting_text: DR   FunFam; 3.10.110.10:FF:000035; SUMO-conjugating enzyme ubc9; 1.
    - reference_id: file:NEUCR/NCU04302/NCU04302-bioinformatics/RESULTS.md
      supporting_text: The full-length target sequence is 75.0% identical to fission-yeast Ubc9 P40984
        across 156 aligned residue pairs (99.36% target coverage), and 62.42% identical to human Ubc9
        P63279 across 157 residue pairs (100% target coverage).
    - reference_id: PMID:12597774
      supporting_text: The Hus5.62 protein is also capable of forming a thioester link with SUMO, but
        it does not function in sumoylation assays, either in the modification of Rad22 or in SUMO chain
        formation.
    - reference_id: file:NEUCR/NCU04302/NCU04302-uniprot.txt
      supporting_text: DR   GO; GO:0061656; F:SUMO conjugating enzyme activity; IBA:GO_Central.
references:
- id: file:NEUCR/NCU04302/NCU04302-uniprot.txt
  title: NCU04302-uniprot.txt
  findings: []
- id: PMID:9435231
  title: The ubiquitin-like proteins SMT3 and SUMO-1 are conjugated by the UBC9 E2 enzyme.
  findings: []
  full_text_unavailable: true
- id: file:NEUCR/NCU04302/NCU04302-bioinformatics/RESULTS.md
  title: RESULTS.md
  findings: []
- id: PMID:12597774
  title: Characterization of SUMO-conjugating enzyme mutants in Schizosaccharomyces pombe identifies a
    dominant-negative allele that severely reduces SUMO conjugation.
  findings: []
  full_text_unavailable: true