ProtNLM2 External predictions
View prediction review YAML · NCU04937-protnlm-predictions-review.yaml · Review status: COMPLETE
DNA binding is uncertain for this uncharacterised orphan ORF, whose sequence is dominated by a 50-residue polyglutamine run and which carries no DNA-binding domain.
Source documents: genes/NEUCR/NCU04937/NCU04937-protnlm-source.json · genes/NEUCR/NCU04937/NCU04937-uniprot.txt
Review score: 2 = concordant with evidence; 1 = uncertain; 0 = discordant with evidence. This is an assessment score, not a model probability.
UNC — Uncertain Review score: 1/2
Prediction method: ProtNLM2 · Version: UniProt API snapshot 2026-09-09 · file:NEUCR/NCU04937/NCU04937-protnlm-source.json
Review rationale: NCU04937 is an uncharacterised 114-residue orphan ORF in which a single run of 50 glutamines (residues 55-104) makes up about half the sequence, under a predicted coiled coil. It has no conserved domain; its only family signature, a weak PANTHER PTHR13270:SF14 match, lies over the polyglutamine run and reflects low-complexity sequence rather than a shared domain. The prediction cites human THAP11 (Q96EK4; phmmer score 29.1), whose DNA binding resides in its THAP zinc-finger domain, which NCU04937 lacks; shared glutamine-rich content does not transfer that function. The gene has no GOA annotations, no ortholog was detected in other Neurospora species, and a PubMed search for NCU04937 returned no publications. Nothing supports or refutes DNA binding, so the prediction is uncertain.
Supporting Evidence:
- file:NEUCR/NCU04937/NCU04937-uniprot.txt: "FT COILED 52..104"
- file:NEUCR/NCU04937/NCU04937-bioinformatics/RESULTS-rereview.md: "The weak PANTHER score and the composition of the matched region show that the assignment comes from low-complexity sequence and not from a shared conserved domain."