NCU07379 encodes an AP-1-like bZIP transcription factor related to fungal Yap1 proteins. Conserved family placement supports binding to transcriptional regulatory DNA and activation of RNA polymerase II transcription in the nucleus. Its physiological target genes and regulatory inputs in Neurospora crassa remain incompletely characterized.
Existing Annotations Review
GO Term
Evidence
Action
Reason
GO:0000976 transcription cis-regulatory region binding
IBA GO_REF:0000033
ACCEPT
Summary: The target is placed in the fungal AP-1/Yap1-related bZIP subfamily.
Reason: The target is placed in the fungal AP-1/Yap1-related bZIP subfamily. Characterized yeast Yap1 binds a cis-regulatory sequence and is required for element-dependent transcriptional activation; the curated PAINT inference supports conservation of this regulatory activity without specifying the Neurospora target genes.
Supporting Evidence:
file:NEUCR/NCU07379/NCU07379-uniprot.txt
DR PANTHER; PTHR40621:SF6; AP-1-LIKE TRANSCRIPTION FACTOR YAP1-RELATED; 1.
Disruption of the YAP1 gene demonstrates this gene is not essential but is required for AP-1 recognition element-dependent transcriptional activation.
GO:0000976 transcription cis-regulatory region binding
IEA GO_REF:0000002
ACCEPT
Summary: The target is placed in the fungal AP-1/Yap1-related bZIP subfamily.
Reason: The target is placed in the fungal AP-1/Yap1-related bZIP subfamily. Characterized yeast Yap1 binds a cis-regulatory sequence and is required for element-dependent transcriptional activation; the curated PAINT inference supports conservation of this regulatory activity without specifying the Neurospora target genes.
Supporting Evidence:
file:NEUCR/NCU07379/NCU07379-uniprot.txt
DR PANTHER; PTHR40621:SF6; AP-1-LIKE TRANSCRIPTION FACTOR YAP1-RELATED; 1.
Summary: The target is placed in the fungal AP-1/Yap1-related bZIP subfamily.
Reason: The target is placed in the fungal AP-1/Yap1-related bZIP subfamily. Characterized yeast Yap1 binds a cis-regulatory sequence and is required for element-dependent transcriptional activation; the curated PAINT inference supports conservation of this regulatory activity without specifying the Neurospora target genes.
Supporting Evidence:
file:NEUCR/NCU07379/NCU07379-uniprot.txt
DR PANTHER; PTHR40621:SF6; AP-1-LIKE TRANSCRIPTION FACTOR YAP1-RELATED; 1.
Summary: The target is placed in the fungal AP-1/Yap1-related bZIP subfamily.
Reason: The target is placed in the fungal AP-1/Yap1-related bZIP subfamily. Characterized yeast Yap1 binds a cis-regulatory sequence and is required for element-dependent transcriptional activation; the curated PAINT inference supports conservation of this regulatory activity without specifying the Neurospora target genes.
Supporting Evidence:
file:NEUCR/NCU07379/NCU07379-uniprot.txt
DR PANTHER; PTHR40621:SF6; AP-1-LIKE TRANSCRIPTION FACTOR YAP1-RELATED; 1.
Summary: A nuclear site of action follows from the supported DNA-binding transcription factor function and bZIP-family placement.
Reason: A nuclear site of action follows from the supported DNA-binding transcription factor function and bZIP-family placement. Constitutive nuclear accumulation or the stimulus-dependent import/export mechanism is not established.
Supporting Evidence:
file:NEUCR/NCU07379/NCU07379-uniprot.txt
DR PANTHER; PTHR40621:SF6; AP-1-LIKE TRANSCRIPTION FACTOR YAP1-RELATED; 1.
Disruption of the YAP1 gene demonstrates this gene is not essential but is required for AP-1 recognition element-dependent transcriptional activation.
GO:0006355 regulation of DNA-templated transcription
IEA GO_REF:0000002
ACCEPT
Summary: The target is placed in the fungal AP-1/Yap1-related bZIP subfamily.
Reason: The target is placed in the fungal AP-1/Yap1-related bZIP subfamily. Characterized yeast Yap1 binds a cis-regulatory sequence and is required for element-dependent transcriptional activation; the curated PAINT inference supports conservation of this regulatory activity without specifying the Neurospora target genes.
Supporting Evidence:
file:NEUCR/NCU07379/NCU07379-uniprot.txt
DR PANTHER; PTHR40621:SF6; AP-1-LIKE TRANSCRIPTION FACTOR YAP1-RELATED; 1.
Disruption of the YAP1 gene demonstrates this gene is not essential but is required for AP-1 recognition element-dependent transcriptional activation.
GO:0045944 positive regulation of transcription by RNA polymerase II
IEA GO_REF:0000108
ACCEPT
Summary: The target is placed in the fungal AP-1/Yap1-related bZIP subfamily.
Reason: The target is placed in the fungal AP-1/Yap1-related bZIP subfamily. Characterized yeast Yap1 binds a cis-regulatory sequence and is required for element-dependent transcriptional activation; the curated PAINT inference supports conservation of this regulatory activity without specifying the Neurospora target genes.
Supporting Evidence:
file:NEUCR/NCU07379/NCU07379-uniprot.txt
DR PANTHER; PTHR40621:SF6; AP-1-LIKE TRANSCRIPTION FACTOR YAP1-RELATED; 1.
Disruption of the YAP1 gene demonstrates this gene is not essential but is required for AP-1 recognition element-dependent transcriptional activation.
GO:0090575 RNA polymerase II transcription regulator complex
IBA GO_REF:0000033
ACCEPT
Summary: A DNA-binding bZIP transcription activator acts in a transcription regulator assembly, and PAINT supports inheritance of this complex association.
Reason: A DNA-binding bZIP transcription activator acts in a transcription regulator assembly, and PAINT supports inheritance of this complex association. The annotation does not identify a specific heterodimer partner or imply membership in core RNA polymerase II.
Supporting Evidence:
file:NEUCR/NCU07379/NCU07379-uniprot.txt
DR PANTHER; PTHR40621:SF6; AP-1-LIKE TRANSCRIPTION FACTOR YAP1-RELATED; 1.
These computational predictions are reviewed separately from the GOA annotation set used for this review. The assessments below are from this project and do not constitute official GO annotations or endorsement by GO/UniProt. They are not included in the existing annotation review above.
Review rationale: The target has a bZIP superfamily assignment and is placed specifically with fungal AP-1/Yap1-related transcription factors. Characterized yeast Yap1 is required for cis-element-dependent transcriptional activation, supporting a conserved DNA-binding regulator function. GOA already includes the more specific RNA polymerase II transcription activator activity by PAINT. The generic transcription-factor prediction is therefore less precise, without implying an established Neurospora stress response or target regulon.
PMID:2542125: "Disruption of the YAP1 gene demonstrates this gene is not essential but is required for AP-1 recognition element-dependent transcriptional activation."