NCU08595 is a Rpf2-family ribosome assembly factor with a Brix RNA-binding domain. By conservation with characterized fungal Rpf2, it associates with Rrs1, binds 5S rRNA and promotes incorporation of the 5S ribonucleoprotein particle into nucleolar large-subunit precursors. This assembly step supports large-subunit rRNA maturation.
Summary: NCU08595 has the Rpf2-specific family assignment in addition to its Brix domain.
Reason: NCU08595 has the Rpf2-specific family assignment in addition to its Brix domain. Characterized fungal Rpf2-Rrs1 recruits 5S ribonucleoprotein components into pre-ribosomes and supports subsequent large-subunit rRNA maturation. This is conserved assembly-factor participation, not intrinsic rRNA cleavage.
gel shift analysis revealed that the Rpf2-Rrs1 complex binds directly to 5S rRNA.
GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
IBA GO_REF:0000033
ACCEPT
Summary: NCU08595 has the Rpf2-specific family assignment in addition to its Brix domain.
Reason: NCU08595 has the Rpf2-specific family assignment in addition to its Brix domain. Characterized fungal Rpf2-Rrs1 recruits 5S ribonucleoprotein components into pre-ribosomes and supports subsequent large-subunit rRNA maturation. This is conserved assembly-factor participation, not intrinsic rRNA cleavage.
Summary: NCU08595 has the Rpf2-specific family assignment in addition to its Brix domain.
Reason: NCU08595 has the Rpf2-specific family assignment in addition to its Brix domain. Characterized fungal Rpf2-Rrs1 recruits 5S ribonucleoprotein components into pre-ribosomes and supports subsequent large-subunit rRNA maturation. This is conserved assembly-factor participation, not intrinsic rRNA cleavage.
Summary: Rpf2 assembles 5S ribonucleoprotein components into nuclear pre-ribosomes and supports early large-subunit maturation.
Reason: Rpf2 assembles 5S ribonucleoprotein components into nuclear pre-ribosomes and supports early large-subunit maturation. The Rpf2-specific family and curator-assessed ancestral localization justify nucleolar residence by conservation.
Summary: Rpf2 assembles 5S ribonucleoprotein components into nuclear pre-ribosomes and supports early large-subunit maturation.
Reason: Rpf2 assembles 5S ribonucleoprotein components into nuclear pre-ribosomes and supports early large-subunit maturation. The Rpf2-specific family and curator-assessed ancestral localization justify nucleolar residence by conservation.
Summary: The broad ribosome-biogenesis or RNA-processing assertion is supported but can be made precise: Rpf2 supports large-subunit rRNA maturation through recruitment and assembly of the 5S ribonucleoprotein particle..
Reason: The broad ribosome-biogenesis or RNA-processing assertion is supported but can be made precise: Rpf2 supports large-subunit rRNA maturation through recruitment and assembly of the 5S ribonucleoprotein particle.
Summary: Gel-shift and mutational analyses of fungal Rpf2-Rrs1 establish direct 5S rRNA binding with a contribution from Rpf2.
Reason: Gel-shift and mutational analyses of fungal Rpf2-Rrs1 establish direct 5S rRNA binding with a contribution from Rpf2. The target Rpf2-specific domain supports transfer of this RNA-binding property beyond the generic Brix fold.
Summary: Gel-shift and mutational analyses of fungal Rpf2-Rrs1 establish direct 5S rRNA binding with a contribution from Rpf2.
Reason: Gel-shift and mutational analyses of fungal Rpf2-Rrs1 establish direct 5S rRNA binding with a contribution from Rpf2. The target Rpf2-specific domain supports transfer of this RNA-binding property beyond the generic Brix fold.
Summary: The broad ribosome-biogenesis or RNA-processing assertion is supported but can be made precise: Rpf2 supports large-subunit rRNA maturation through recruitment and assembly of the 5S ribonucleoprotein particle..
Reason: The broad ribosome-biogenesis or RNA-processing assertion is supported but can be made precise: Rpf2 supports large-subunit rRNA maturation through recruitment and assembly of the 5S ribonucleoprotein particle.
gel shift analysis revealed that the Rpf2-Rrs1 complex binds directly to 5S rRNA.
Core Functions
NCU08595 is a Rpf2-family ribosome assembly factor with a Brix RNA-binding domain. By conservation with characterized fungal Rpf2, it associates with Rrs1, binds 5S rRNA and promotes incorporation of the 5S ribonucleoprotein particle into nucleolar large-subunit precursors. This assembly step supports large-subunit rRNA maturation.
These computational predictions are reviewed separately from the GOA annotation set used for this review. The assessments below are from this project and do not constitute official GO annotations or endorsement by GO/UniProt. They are not included in the existing annotation review above.
NCU08595 is a Rpf2-family ribosome assembly factor with a Brix RNA-binding domain. By conservation with characterized fungal Rpf2, it associates with Rrs1, binds 5S rRNA and promotes incorporation of the 5S ribonucleoprotein particle into nucleolar large-subunit precursors. This assembly step supports large-subunit rRNA maturation.
Review rationale: The Rpf2-specific family assignment, beyond the generic Brix domain, supports transfer from characterized fungal Rpf2-Rrs1 complexes. Structural and biochemical studies establish 5S rRNA recruitment and downstream large-subunit maturation. GOA already includes maturation of LSU-rRNA from the tricistronic precursor (GO:0000463), making this general LSU-rRNA maturation prediction less precise. Participation is through ribosome assembly and does not imply intrinsic nuclease chemistry.
Supporting Evidence:
PMID:25855814: "Proteins Rpf2 and Rrs1 are required for 60S ribosomal subunit maturation."
PMID:25855814: "gel shift analysis revealed that the Rpf2-Rrs1 complex binds directly to 5S rRNA."