id: Q7SCN3 gene_symbol: NCU08595 product_type: PROTEIN status: COMPLETE taxon: id: NCBITaxon:367110 label: Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) description: NCU08595 is a Rpf2-family ribosome assembly factor with a Brix RNA-binding domain. By conservation with characterized fungal Rpf2, it associates with Rrs1, binds 5S rRNA and promotes incorporation of the 5S ribonucleoprotein particle into nucleolar large-subunit precursors. This assembly step supports large-subunit rRNA maturation. existing_annotations: - term: &id002 id: GO:0000027 label: ribosomal large subunit assembly evidence_type: IEA original_reference_id: GO_REF:0000002 qualifier: involved_in supporting_entities: - InterPro:IPR039770 review: summary: NCU08595 has the Rpf2-specific family assignment in addition to its Brix domain. action: ACCEPT reason: NCU08595 has the Rpf2-specific family assignment in addition to its Brix domain. Characterized fungal Rpf2-Rrs1 recruits 5S ribonucleoprotein components into pre-ribosomes and supports subsequent large-subunit rRNA maturation. This is conserved assembly-factor participation, not intrinsic rRNA cleavage. supported_by: - reference_id: PMID:25855814 supporting_text: Proteins Rpf2 and Rrs1 are required for 60S ribosomal subunit maturation. - reference_id: PMID:25855814 supporting_text: 'gel shift analysis revealed that the Rpf2-Rrs1 complex binds directly to 5S rRNA.' - term: &id001 id: GO:0000463 label: maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) evidence_type: IBA original_reference_id: GO_REF:0000033 qualifier: involved_in supporting_entities: - PANTHER:PTN000296192 - SGD:S000001789 review: summary: NCU08595 has the Rpf2-specific family assignment in addition to its Brix domain. action: ACCEPT reason: NCU08595 has the Rpf2-specific family assignment in addition to its Brix domain. Characterized fungal Rpf2-Rrs1 recruits 5S ribonucleoprotein components into pre-ribosomes and supports subsequent large-subunit rRNA maturation. This is conserved assembly-factor participation, not intrinsic rRNA cleavage. supported_by: - reference_id: PMID:25855814 supporting_text: Proteins Rpf2 and Rrs1 are required for 60S ribosomal subunit maturation. - reference_id: PMID:25855814 supporting_text: 'gel shift analysis revealed that the Rpf2-Rrs1 complex binds directly to 5S rRNA.' - term: id: GO:0000470 label: maturation of LSU-rRNA evidence_type: IEA original_reference_id: GO_REF:0000002 qualifier: involved_in supporting_entities: - InterPro:IPR039770 review: summary: NCU08595 has the Rpf2-specific family assignment in addition to its Brix domain. action: ACCEPT reason: NCU08595 has the Rpf2-specific family assignment in addition to its Brix domain. Characterized fungal Rpf2-Rrs1 recruits 5S ribonucleoprotein components into pre-ribosomes and supports subsequent large-subunit rRNA maturation. This is conserved assembly-factor participation, not intrinsic rRNA cleavage. supported_by: - reference_id: PMID:25855814 supporting_text: Proteins Rpf2 and Rrs1 are required for 60S ribosomal subunit maturation. - reference_id: PMID:25855814 supporting_text: 'gel shift analysis revealed that the Rpf2-Rrs1 complex binds directly to 5S rRNA.' - term: id: GO:0005730 label: nucleolus evidence_type: IBA original_reference_id: GO_REF:0000033 qualifier: is_active_in supporting_entities: - AGI_LocusCode:AT3G23620 - FB:FBgn0038585 - PANTHER:PTN000296192 - SGD:S000001789 - UniProtKB:Q8IJC0 - UniProtKB:Q9H7B2 review: summary: Rpf2 assembles 5S ribonucleoprotein components into nuclear pre-ribosomes and supports early large-subunit maturation. action: ACCEPT reason: Rpf2 assembles 5S ribonucleoprotein components into nuclear pre-ribosomes and supports early large-subunit maturation. The Rpf2-specific family and curator-assessed ancestral localization justify nucleolar residence by conservation. supported_by: - reference_id: PMID:25855814 supporting_text: Proteins Rpf2 and Rrs1 are required for 60S ribosomal subunit maturation. - term: &id004 id: GO:0005730 label: nucleolus evidence_type: IEA original_reference_id: GO_REF:0000120 qualifier: located_in supporting_entities: - UniProtKB-SubCell:SL-0188 - UniRule:UR001419758 review: summary: Rpf2 assembles 5S ribonucleoprotein components into nuclear pre-ribosomes and supports early large-subunit maturation. action: ACCEPT reason: Rpf2 assembles 5S ribonucleoprotein components into nuclear pre-ribosomes and supports early large-subunit maturation. The Rpf2-specific family and curator-assessed ancestral localization justify nucleolar residence by conservation. supported_by: - reference_id: PMID:25855814 supporting_text: Proteins Rpf2 and Rrs1 are required for 60S ribosomal subunit maturation. - term: id: GO:0006364 label: rRNA processing evidence_type: IEA original_reference_id: GO_REF:0000002 qualifier: involved_in supporting_entities: - InterPro:IPR007109 review: summary: 'The broad ribosome-biogenesis or RNA-processing assertion is supported but can be made precise: Rpf2 supports large-subunit rRNA maturation through recruitment and assembly of the 5S ribonucleoprotein particle..' action: MODIFY reason: 'The broad ribosome-biogenesis or RNA-processing assertion is supported but can be made precise: Rpf2 supports large-subunit rRNA maturation through recruitment and assembly of the 5S ribonucleoprotein particle.' supported_by: - reference_id: PMID:25855814 supporting_text: Proteins Rpf2 and Rrs1 are required for 60S ribosomal subunit maturation. - reference_id: PMID:25855814 supporting_text: 'gel shift analysis revealed that the Rpf2-Rrs1 complex binds directly to 5S rRNA.' proposed_replacement_terms: - id: GO:0000463 label: maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) - term: id: GO:0019843 label: rRNA binding evidence_type: IBA original_reference_id: GO_REF:0000033 qualifier: enables supporting_entities: - AGI_LocusCode:AT3G23620 - PANTHER:PTN000296192 - SGD:S000001789 - UniProtKB:Q57U27 - UniProtKB:Q9H7B2 review: summary: Gel-shift and mutational analyses of fungal Rpf2-Rrs1 establish direct 5S rRNA binding with a contribution from Rpf2. action: ACCEPT reason: Gel-shift and mutational analyses of fungal Rpf2-Rrs1 establish direct 5S rRNA binding with a contribution from Rpf2. The target Rpf2-specific domain supports transfer of this RNA-binding property beyond the generic Brix fold. supported_by: - reference_id: PMID:25855814 supporting_text: 'gel shift analysis revealed that the Rpf2-Rrs1 complex binds directly to 5S rRNA.' - term: &id003 id: GO:0019843 label: rRNA binding evidence_type: IEA original_reference_id: GO_REF:0000120 qualifier: enables supporting_entities: - InterPro:IPR007109 - InterPro:IPR039770 - UniRule:UR001419758 review: summary: Gel-shift and mutational analyses of fungal Rpf2-Rrs1 establish direct 5S rRNA binding with a contribution from Rpf2. action: ACCEPT reason: Gel-shift and mutational analyses of fungal Rpf2-Rrs1 establish direct 5S rRNA binding with a contribution from Rpf2. The target Rpf2-specific domain supports transfer of this RNA-binding property beyond the generic Brix fold. supported_by: - reference_id: PMID:25855814 supporting_text: 'gel shift analysis revealed that the Rpf2-Rrs1 complex binds directly to 5S rRNA.' - term: id: GO:0042254 label: ribosome biogenesis evidence_type: IEA original_reference_id: GO_REF:0000117 qualifier: involved_in supporting_entities: - ARBA:ARBA00026817 review: summary: 'The broad ribosome-biogenesis or RNA-processing assertion is supported but can be made precise: Rpf2 supports large-subunit rRNA maturation through recruitment and assembly of the 5S ribonucleoprotein particle..' action: MODIFY reason: 'The broad ribosome-biogenesis or RNA-processing assertion is supported but can be made precise: Rpf2 supports large-subunit rRNA maturation through recruitment and assembly of the 5S ribonucleoprotein particle.' supported_by: - reference_id: PMID:25855814 supporting_text: Proteins Rpf2 and Rrs1 are required for 60S ribosomal subunit maturation. - reference_id: PMID:25855814 supporting_text: 'gel shift analysis revealed that the Rpf2-Rrs1 complex binds directly to 5S rRNA.' proposed_replacement_terms: - id: GO:0000463 label: maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) references: - id: GO_REF:0000002 title: Gene Ontology annotation through association of InterPro records with GO terms findings: [] - id: GO_REF:0000033 title: Annotation inferences using phylogenetic trees findings: [] - id: GO_REF:0000117 title: Electronic Gene Ontology annotations created by ARBA machine learning models findings: [] - id: GO_REF:0000120 title: Combined Automated Annotation using Multiple IEA Methods findings: [] - id: PMID:25855814 title: Structural and functional analysis of the Rpf2-Rrs1 complex in ribosome biogenesis. full_text_unavailable: false - id: file:NEUCR/NCU08595/NCU08595-uniprot.txt title: 'UniProt record for NCU08595: domain and family evidence' core_functions: - description: NCU08595 is a Rpf2-family ribosome assembly factor with a Brix RNA-binding domain. By conservation with characterized fungal Rpf2, it associates with Rrs1, binds 5S rRNA and promotes incorporation of the 5S ribonucleoprotein particle into nucleolar large-subunit precursors. This assembly step supports large-subunit rRNA maturation. supported_by: - reference_id: PMID:25855814 supporting_text: Proteins Rpf2 and Rrs1 are required for 60S ribosomal subunit maturation. - reference_id: PMID:25855814 supporting_text: 'gel shift analysis revealed that the Rpf2-Rrs1 complex binds directly to 5S rRNA.' - reference_id: file:NEUCR/NCU08595/NCU08595-uniprot.txt supporting_text: DR InterPro; IPR039770; Rpf2. directly_involved_in: - *id001 - *id002 molecular_function: *id003 locations: - *id004